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Identification Of The Flii And Flij Components Of The Caulobacter Flagellar Type Iii Protein Secretion System, Craig Stephens, Chris Mohr, Charles Boyd, Janine Maddock, James Gober, Lucy Shapiro
Identification Of The Flii And Flij Components Of The Caulobacter Flagellar Type Iii Protein Secretion System, Craig Stephens, Chris Mohr, Charles Boyd, Janine Maddock, James Gober, Lucy Shapiro
Biology
Caulobacter crescentus is motile by virtue of a polar flagellum assembled during the predivisional stage of the cell cycle. Three mutant strains in which flagellar assembly was blocked at an early stage were isolated. The mutations in these strains mapped to an operon of two genes, fliI and fliJ, both of which are necessary for motility. fliI encodes a 50-kDa polypeptide whose sequence is closely related to that of the Salmonella typhimurium FliI protein, an ATPase thought to energize the export of flagellar subunits across the cytoplasmic membrane through a type III protein secretion system. fliJ encodes a 16-kDa hydrophilic …