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University of Nebraska - Lincoln
TMV-based vectors; Recombinant protein; Bovine herpes virus-1 (BHV-1)
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Full-Text Articles in Life Sciences
Bovine Herpes Virus Gd Protein Produced In Plants Using A Recombinant Tobacco Mosaic Virus (Tmv) Vector Possesses Authentic Antigenicity, D. M. Pérez Filgueira, P.I. Zamorano, M. G. Dominguez, O. Taboga, M. P. Del Medico Zajac, M. Puntel, S. A. Romera, Thomas Jack Morris, M. V. Borca, A. M. Sadir
Bovine Herpes Virus Gd Protein Produced In Plants Using A Recombinant Tobacco Mosaic Virus (Tmv) Vector Possesses Authentic Antigenicity, D. M. Pérez Filgueira, P.I. Zamorano, M. G. Dominguez, O. Taboga, M. P. Del Medico Zajac, M. Puntel, S. A. Romera, Thomas Jack Morris, M. V. Borca, A. M. Sadir
Papers in Virology
A tobacco mosaic virus (TMV)-based vector was utilized for expression of a cytosolic form of the bovine herpesvirus type 1 (BHV-1) protein glycoprotein D (gDc). Nicotiana benthamiana plants were harvested 7 days after inoculation with RNA transcripts derived from the TMV-gDc recombinant virus. Recombinant gDc protein of expected electrophoretic mobility accumulated in inoculated leaves to a concentration of about 20 μg/g of fresh leaf tissue. Oil-based vaccines were formulated with crude foliar extracts to immunize mice parentally. After a single injection, animals developed a sustained and specific response to both the isolated gD and native virus particles. Cattle vaccinated with …