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- Chlorella viruses (2)
- Coloniality (2)
- Genome sequence (2)
- Oral delivery; enteric coatings; adenoviral vectors; HIV-1 peptides; mucosal vaccination (2)
- Phycodnaviridae (2)
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- Virus ecology (2)
- <i>Gryllus assimilis</i> (1)
- 13C (1)
- 15N (1)
- AIDS viral oncogenesis (1)
- Arbovirus (1)
- Bird movement (1)
- Buggy Creek virus (1)
- Cliff swallow (1)
- Ecological intensification (1)
- HHV-8/KSHV (1)
- HHV8 (1)
- HIV (1)
- HIV-1; vaccine; neutralization antibody; consensus immunogens (1)
- Hemimetabolous insect (1)
- Human herpesvirus 8 (1)
- Humic acids (1)
- Indel polymorphism (1)
- Indigenous soil N supply (1)
- Juvenile hormone esterase gene (1)
- KSHV (1)
- Kaposi's sarcoma (1)
- Kaposi’s sarcoma (1)
- Light fraction (1)
- N mineralization (1)
Articles 1 - 30 of 43
Full-Text Articles in Life Sciences
Oral Immunization Of Rhesus Macaques With Adenoviral Hiv Vaccines Using Enteric-Coated Capsules, George T. Mercier, Pramod N. Nehete, Marco F. Passeri, Bharti N. Nehete, Eric A. Weaver, Nancy Smyth Templeton, Kimberly Schluns, Stephanie S. Buchl, K. Buchl, Michael A. Barry
Oral Immunization Of Rhesus Macaques With Adenoviral Hiv Vaccines Using Enteric-Coated Capsules, George T. Mercier, Pramod N. Nehete, Marco F. Passeri, Bharti N. Nehete, Eric A. Weaver, Nancy Smyth Templeton, Kimberly Schluns, Stephanie S. Buchl, K. Buchl, Michael A. Barry
Nebraska Center for Virology: Faculty Publications
Targeted delivery of vaccine candidates to the gastrointestinal (GI) tract holds potential for mucosal immunization, particularly against mucosal pathogens like the human immunodeficiency virus (HIV). Among the different strategies for achieving targeted release in the GI tract, namely the small intestine, pH sensitive enteric coating polymers have been shown to protect solid oral dosage forms from the harsh digestive environment of the stomach and dissolve relatively rapidly in the small intestine by taking advantage of the luminal pH gradient. We developed an enteric polymethacrylate formulation for coating hydroxy-propyl-methyl-cellulose (HPMC) capsules containing lyophilized Adenoviral type 5 (Ad5) vectors expressing HIV-1 gag …
Jhe In Gryllus Assimilis: Cloning, Sequence-Activity Associations And Phylogeny, E. J. Crone, Anthony J. Zera, A. Anand, J. G. Oakeshott, T. D. Sutherland, R. J. Russell, Lawrence G. Harshman, Federico G. Hoffmann, C. Claudianos
Jhe In Gryllus Assimilis: Cloning, Sequence-Activity Associations And Phylogeny, E. J. Crone, Anthony J. Zera, A. Anand, J. G. Oakeshott, T. D. Sutherland, R. J. Russell, Lawrence G. Harshman, Federico G. Hoffmann, C. Claudianos
Anthony Zera Publications
The 458 amino acid sequence of a mature JHE protein from the cricket Gryllus assimilis was identified after isolating the partial cDNA sequence encoding this protein from a fat body and midgut cDNA library. This hemimetabolan JHE sequence shows over 40% amino acid similarity to the known JHE sequences of several holometabolous insects. It also includes previously determined peptide sequences for G. assimilis JHE as well as two other motifs associated with JHE enzymes in holometabolous insects. The predicted molecular weight of the protein agrees with that of the JHE previously purified from G. assimilis. Partial genomic sequence encoding …
Sheeppox Virus Kelch-Like Gene Sppv-019 Affects Virus Virulence, C. A. Balinsky, Gustavo A. Delhon, C. L. Afonso, G. R. Risatti, M. V. Borca, R. A. French, E. R. Tulman, S. J. Geary, D. L. Rock
Sheeppox Virus Kelch-Like Gene Sppv-019 Affects Virus Virulence, C. A. Balinsky, Gustavo A. Delhon, C. L. Afonso, G. R. Risatti, M. V. Borca, R. A. French, E. R. Tulman, S. J. Geary, D. L. Rock
Nebraska Center for Virology: Faculty Publications
Sheeppox virus (SPPV), a member of the Capripoxvirus genus of the Poxviridae, is the etiologic agent of a significant disease of sheep in the developing world. Genomic analysis of pathogenic and vaccine capripoxviruses identified genes with potential roles in virulence and host range, including three genes with similarity to kelch-like genes of other poxviruses and eukaryotes. Here, a mutant SPPV with a deletion in the SPPV-019 kelch-like gene, ΔKLP, was derived from the pathogenic strain SPPV-SA. ΔKLP exhibited in vitro growth characteristics similar to those of SPPV-SA and revertant virus (RvKLP). ΔKLP-infected cells exhibited a reduction in Ca2+ …
A Group M Consensus Envelope Glycoprotein Induces Antibodies That Neutralize Subsets Of Subtype B And C Hiv-1 Primary Viruses, Hua-Xin Liao, Laura L. Sutherland, Shi-Mao Xia, Mary E. Brock, Richard M. Scearce, Stacie Vanleeuwen, S. Munir Alam, Mildred Mcadams, Eric A. Weaver, Zenaido T. Camacho, Ben-Jiang Ma, Yingying Li, Julie M. Decker, Gary J. Nabel, David C. Montefiori, Beatrice H. Hahn, Bette T. Korber, Feng Gao, Barton F. Haynes
A Group M Consensus Envelope Glycoprotein Induces Antibodies That Neutralize Subsets Of Subtype B And C Hiv-1 Primary Viruses, Hua-Xin Liao, Laura L. Sutherland, Shi-Mao Xia, Mary E. Brock, Richard M. Scearce, Stacie Vanleeuwen, S. Munir Alam, Mildred Mcadams, Eric A. Weaver, Zenaido T. Camacho, Ben-Jiang Ma, Yingying Li, Julie M. Decker, Gary J. Nabel, David C. Montefiori, Beatrice H. Hahn, Bette T. Korber, Feng Gao, Barton F. Haynes
Nebraska Center for Virology: Faculty Publications
HIV-1 subtype C is the most common HIV-1 group M subtype in Africa and many parts of Asia. However, to date HIV-1 vaccine candidate immunogens have not induced potent and broadly neutralizing antibodies against subtype C primary isolates. We have used a centralized gene strategy to address HIV-1 diversity, and generated a group M consensus envelope gene with shortened consensus variable loops (CON-S) for comparative studies with wildtype (WT) Env immunogens. Our results indicate that the consensus HIV-1 group M CON-S Env elicited cross-subtype neutralizing antibodies of similar or greater breadth and titer than the WT Envs tested, indicating the …
Hormones In The Field: Evolutionary Endocrinology Of Juvenile Hormone And Ecdysteroids In Field Populations Of The Wing-Dimorphic Cricket Gryllus Firmus, Anthony J. Zera, Zangwu Zhao, Katherine Kaliseck
Hormones In The Field: Evolutionary Endocrinology Of Juvenile Hormone And Ecdysteroids In Field Populations Of The Wing-Dimorphic Cricket Gryllus Firmus, Anthony J. Zera, Zangwu Zhao, Katherine Kaliseck
Anthony Zera Publications
Virtually no published information exists on insect endocrine traits in natural populations, which limits our understanding of endocrine microevolution. We characterized the hemolymph titers of juvenile hormone (JH) and ecdysteroids (ECDs), two key insect hormones, in field-collected short-winged, flightless (SW) and long-winged, flight-capable (LW(f)) morphs of the cricket Gryllus firmus. The JH titer exhibited a dramatic circadian rhythm in the LW(f) morph but was temporally constant in the flightless SW morph. This pattern was consistent in each of three years; in young, middle-aged, and older G. firmus; and in three other cricket species. The ECD titer was considerably …
The Effect Of Residue C:N Ratio On The Turnover Of N And C In Various Soil Organic Matter Fractions, Ana B. Wingeyer
The Effect Of Residue C:N Ratio On The Turnover Of N And C In Various Soil Organic Matter Fractions, Ana B. Wingeyer
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
Identifying soil organic matter (SOM) fractions that contribute to soil indigenous nitrogen (N) supply and understanding their turnover under different management constitute necessary tools toward an efficient N use. The objectives of this study were: i) trace the endpoint of carbon (C) flux from residue inputs into SOM; and ii) asses the role of the light fraction (LF), mobile humic acid (MHA) fraction and calcium humate (CaHA) fraction as N sources for heterotrophic decomposition of fresh plant residues with contrasting C:N ratio. A long-term aerobic soil incubation was carried out on 15N-labeled soil samples from Lincoln and Mead, NE. …
Exogenous Farnesol Interferes With The Normal Progression Of Cytokine Expression During Candidiasis In A Mouse Model, Dhammika H. M. L. P. Navarathna, Kenneth W. Nickerson, Gerald E. Duhamel, Thomas R. Jerrels, Thomas M. Petro
Exogenous Farnesol Interferes With The Normal Progression Of Cytokine Expression During Candidiasis In A Mouse Model, Dhammika H. M. L. P. Navarathna, Kenneth W. Nickerson, Gerald E. Duhamel, Thomas R. Jerrels, Thomas M. Petro
Papers in Microbiology
Candida albicans, a dimorphic fungus composed of yeast and mycelial forms, is the most common human fungal pathogen. Th1 cytokines such as interleukin-2 (IL-2), gamma interferon (IFN-γ), and tumor necrosis factor alpha (TNF-α), which are induced by macrophage IL-12, are critical to resistance against systemic candidiasis, while Th2 cytokines such as IL-4 and IL-5 are less critical. Farnesol is a quorum-sensing molecule produced by C. albicans that controls the formation of mycelia but is also a virulence factor. To determine whether farnesol enhances the virulence of C. albicans by modulating the production of Th1 and Th2 cytokines, mice were …
The Distribution Of Sexually-Transmitted Human Papillomaviruses In Hiv Positive And Negative Patients In Zambia, Africa, Christopher Ngandwe, John Lowe, Paula J. Richards, Lara Hause, Charles Wood, Peter C. Angeletti
The Distribution Of Sexually-Transmitted Human Papillomaviruses In Hiv Positive And Negative Patients In Zambia, Africa, Christopher Ngandwe, John Lowe, Paula J. Richards, Lara Hause, Charles Wood, Peter C. Angeletti
Nebraska Center for Virology: Faculty Publications
Background: Human Papillomaviruses (HPV) are double-stranded DNA viruses, considered to be the primary etiological agents in cervical intraepithelial neoplasias and cancers. Approximately 15–20 of the 40 mucosal HPVs confer a high-risk of progression of lesions to invasive cancer. In this study, we investigated the prevalence of sexually transmitted HPVs in Human Immunodeficiency Virus (HIV) positive and negative patients in Zambia, Africa. The rate of high-risk HPV genotypes worldwide varies within each country. Thus, we sought to investigate the rates of HPV infection in sub-Saharan Africa and the potential role of HIV in affecting the HPV genotype distribution.
Methods: This retrospective …
Chloroviruses Encode A Bifunctional Dcmp-Dctp Deaminase That Produces Two Key Intermediates In Dttp Formation, Yuanzheng Zhang, Frank Maley, Gladys F. Maley, Garry Duncan, David Dunigan, James L. Van Etten
Chloroviruses Encode A Bifunctional Dcmp-Dctp Deaminase That Produces Two Key Intermediates In Dttp Formation, Yuanzheng Zhang, Frank Maley, Gladys F. Maley, Garry Duncan, David Dunigan, James L. Van Etten
Nebraska Center for Virology: Faculty Publications
The chlorovirus PBCV-1, like many large double-stranded DNA-containing viruses, contains several genes that encode putative proteins involved in nucleotide biosynthesis. This report describes the characterization of the PBCV-1 dCMP deaminase, which produces dUMP, a key intermediate in the synthesis of dTTP. As predicted, the recombinant protein has dCMP deaminase activity that is activated by dCTP and inhibited by dTTP. Unexpectedly, however, the viral enzyme also has dCTP deaminase activity, producing dUTP. Typically, these two reactions are catalyzed by proteins in separate enzyme classes; to our knowledge, this is the first example of a protein having both deaminase activities. Kinetic experiments …
The Transcriptional Repressor K-Rbp Modulates Rta-Mediated Transactivation And Lytic Replication Of Kaposi’S Sarcoma-Associated Herpesvirus, Zhilong Yang, Charles Wood
The Transcriptional Repressor K-Rbp Modulates Rta-Mediated Transactivation And Lytic Replication Of Kaposi’S Sarcoma-Associated Herpesvirus, Zhilong Yang, Charles Wood
Nebraska Center for Virology: Faculty Publications
The replication and transcription activator (RTA) protein of Kaposi’s sarcoma (KS)-associated herpesvirus (KSHV)/human herpesvirus 8 functions as the key regulator to induce KSHV lytic replication from latency through activation of the lytic cascade of KSHV. Elucidation of the host factors involved in RTA-mediated transcriptional activation is pivotal for understanding the transition between viral latency and lytic replication. KSHV-RTA binding protein (K-RBP) was previously isolated as a cellular RTA binding protein of unknown function. Sequence analysis showed that K-RBP contains a Kruppel-associated box (KRAB) at the N terminus and 12 adjacent zinc finger motifs. In similarity to other KRAB-containing zinc finger …
Epstein-Barr Virus Inhibits Kaposi’S Sarcoma-Associated Herpesvirus Lytic Replication In Primary Effusion Lymphomas, Dongsheng Xu, Jun Zhang, Tricia Coleman, Ashley Fagot, Catherine Kotalik, Lingjun Zhao, Pankaj Trivedi, Clinton J. Jones, Luwen Zhang
Epstein-Barr Virus Inhibits Kaposi’S Sarcoma-Associated Herpesvirus Lytic Replication In Primary Effusion Lymphomas, Dongsheng Xu, Jun Zhang, Tricia Coleman, Ashley Fagot, Catherine Kotalik, Lingjun Zhao, Pankaj Trivedi, Clinton J. Jones, Luwen Zhang
Nebraska Center for Virology: Faculty Publications
The majority of AIDS-associated primary effusion lymphomas (PEL) are latently infected with both Kaposi’s sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV). PELs harboring two viruses have higher oncogenic potential, suggesting functional interactions between EBV and KSHV. The KSHV replication and transcription activator (K-RTA) is necessary and sufficient for induction of KSHV lytic replication. EBV latent membrane protein 1 (LMP-1) is essential for EBV transformation and establishment of latency in vitro. We show EBV inhibits chemically induced KSHV lytic replication, in part because of a regulatory loop in which K-RTA induces EBV LMP-1 and LMP-1 in turn inhibits K-RTA expression and …
The Nasal Cavity Is A Route For Prion Infection In Hamsters, Anthony E. Kincaid, Jason C. Bartz
The Nasal Cavity Is A Route For Prion Infection In Hamsters, Anthony E. Kincaid, Jason C. Bartz
Nebraska Center for Virology: Faculty Publications
Animals that naturally acquire the prion diseases have a well-developed olfactory sense that they utilize for a variety of basic behaviors. To assess the potential for the nasal cavity to serve as a point of entry for prion diseases, a small amount of prion-infected brain homogenate was placed inferior to the nostrils of hamsters, where it was immediately sniffed into the nasal cavity. Hamsters extranasally inoculated with the HY strain of transmissible mink encephalopathy (TME) agent had an incubation period that was not significantly different from per os inoculation of the same dose of the HY TME agent. However, the …
Effect Of Farnesol On A Mouse Model Of Systemic Candidiasis, Determined By Use Of A Dpp3 Knockout Mutant Of Candida Albicans, Dhammika H.M.L.P. Navarathna, Jacob M. Hornby, Navasona Krishnan, Anne M. Parkhurst, Gerald E. Duhamel, Kenneth W. Nickerson
Effect Of Farnesol On A Mouse Model Of Systemic Candidiasis, Determined By Use Of A Dpp3 Knockout Mutant Of Candida Albicans, Dhammika H.M.L.P. Navarathna, Jacob M. Hornby, Navasona Krishnan, Anne M. Parkhurst, Gerald E. Duhamel, Kenneth W. Nickerson
Papers in Microbiology
This work extends our previous observation that the fungus Candida albicans secretes micromolar levels of farnesol and that accumulation of farnesol in vitro prevents the yeast-to-mycelium conversion in a quorumsensing manner. What does farnesol do in vivo? The purpose of this study was to determine the role of farnesol during infection with a well-established mouse model of systemic candidiasis with C. albicans A72 administered by tail vein injection. This question was addressed by altering both endogenous and exogenous farnesol. For endogenous farnesol, we created a knockout mutation in DPP3, the gene encoding a phosphatase which converts farnesyl pyrophosphate to farnesol. …
In Vivo And In Vitro Anaerobic Mating In Candida Albicans, Raluca Dumitru, Dhammika H.M.L.P. Navarathna, Camile P. Semighini, Christian G. Elowsky, Razvan Dumitru, Daniel Dignard, Malcom Whiteway, Audrey L. Atkin, Kenneth W. Nickerson
In Vivo And In Vitro Anaerobic Mating In Candida Albicans, Raluca Dumitru, Dhammika H.M.L.P. Navarathna, Camile P. Semighini, Christian G. Elowsky, Razvan Dumitru, Daniel Dignard, Malcom Whiteway, Audrey L. Atkin, Kenneth W. Nickerson
Papers in Microbiology
Candida albicans cells of opposite mating types are thought to conjugate during infection in mammalian hosts, but paradoxically, the mating-competent opaque state is not stable at mammalian body temperatures. We found that anaerobic conditions stabilize the opaque state at 37°C, block production of farnesol, and permit in vitro mating at 37°C at efficiencies of up to 84%. Aerobically, farnesol prevents mating because it kills the opaque cells necessary for mating, and as a corollary, farnesol production is turned off in opaque cells. These in vitro observations suggest that naturally anaerobic sites, such as the efficiently colonized gastrointestinal (GI) tract, could …
Protection Against Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Infection Through Passive Transfer Of Prrsv-Neutralizing Antibodies Is Dose Dependent, O. J. Lopez, M. F. Oliveira, E. Alvarez Garcia, Byung Joon Kwon, Alan R. Doster, Fernando A. Osorio
Protection Against Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Infection Through Passive Transfer Of Prrsv-Neutralizing Antibodies Is Dose Dependent, O. J. Lopez, M. F. Oliveira, E. Alvarez Garcia, Byung Joon Kwon, Alan R. Doster, Fernando A. Osorio
Nebraska Center for Virology: Faculty Publications
Previous work in our laboratory demonstrated that passive transfer of porcine reproductive and respiratory syndrome virus (PRRSV)-neutralizing antibodies (NA) protected pregnant sows against reproductive failure and conferred sterilizing immunity in sows and offspring. We report here on the dose requirement for protection by passive transfer with NA in young weaned pigs. The presence of a 1:8 titer of PRRSV-NA in serum consistently protected pigs against viremia. Nevertheless, their lungs, tonsils, buffy coat cells, and peripheral lymph nodes contained replicating PRRSV similar to the infected control group. Likewise, these animals excreted infectious virus to sentinels similar to the infectivity control animals. …
Human Immunodeficiency Virus Type 1 Pathobiology Studied In Humanized Balb/C-Rag2-/- ΓC-/- Mice, Santhi Gorantla, Hannah Seller, Lisa Walters, John G. Sharp, Samuel Pirruccello, John T. West, Charles Wood, Stephen Dewhurst, Howard Gendelman, Larisa Poluektova
Human Immunodeficiency Virus Type 1 Pathobiology Studied In Humanized Balb/C-Rag2-/- ΓC-/- Mice, Santhi Gorantla, Hannah Seller, Lisa Walters, John G. Sharp, Samuel Pirruccello, John T. West, Charles Wood, Stephen Dewhurst, Howard Gendelman, Larisa Poluektova
Nebraska Center for Virology: Faculty Publications
The specificity of human immunodeficiency virus type 1 (HIV-1) for human cells precludes virus infection in most mammalian species and limits the utility of small animal models for studies of disease pathogenesis, therapy, and vaccine development. One way to overcome this limitation is by human cell xenotransplantation in immune-deficient mice. However, this has proved inadequate, as engraftment of human immune cells is limited (both functionally and quantitatively) following transplantation of mature human lymphocytes or fetal thymus/liver. To this end, a human immune system was generated from umbilical cord blood-derived CD34+ hematopoietic stem cells in BALB/c-Rag2-/- γc-/- …
Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten
Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten
Nebraska Center for Virology: Faculty Publications
Viruses MT325 and FR483, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella Pbi. The 314,335-bp genome of MT325 and the 321,240-bp genome of FR483 are the first viruses that infect Chlorella Pbi to have their genomes sequenced and annotated. Furthermore, these genomes are the two smallest chlorella virus genomes sequenced to date, MT325 has 331 putative protein-encoding and 10 tRNA-encoding genes and FR483 has 335 putative protein-encoding and 9 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Approximately 40% of …
Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix A: Gene Names M002r Through M843l, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten
Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix A: Gene Names M002r Through M843l, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten
Nebraska Center for Virology: Faculty Publications
Appendix A: Gene Names m002R through m843L
Document, in spreadsheet format, shows Gene Name, Genome Position, A.A. length, Peptid e Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.
Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix A: Gene Names B002r – B797r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, William C. Nierman, James L. Van Etten
Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix A: Gene Names B002r – B797r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, William C. Nierman, James L. Van Etten
Nebraska Center for Virology: Faculty Publications
Appendix A: Gene Names b002R – b797R
Document shows Gene Name, Genome Position, A.A. length, Peptide Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.
Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix B: Gene Names M001l Through M807r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten
Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix B: Gene Names M001l Through M807r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten
Nebraska Center for Virology: Faculty Publications
Appendix B: Gene Names M001L through M807R
Document, in spreadsheet format, shows Gene Name, Genome Position, A.A. length, Peptid e Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.
Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix C: Gene Names N001l Through N849r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten
Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix C: Gene Names N001l Through N849r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten
Nebraska Center for Virology: Faculty Publications
Appendix C: Gene Names n001L through n849R
Document, in spreadsheet format, shows Gene Name, Genome Position, A.A. length, Peptid e Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.
Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, Willaim C. Nierman, James L. Van Etten
Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, Willaim C. Nierman, James L. Van Etten
Nebraska Center for Virology: Faculty Publications
Viruses NY-2A and AR158, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella NC64A. The 368,683-bp genome of NY-2A and the 344,690-bp genome of AR158 are the two largest chlorella virus genomes sequenced to date; NY-2A contains 404 putative protein-encoding and 7 tRNA-encoding genes and AR158 contains 360 putative protein-encoding and 6 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Two of the NY-2A genes encode inteins, the large subunit of ribonucleotide reductase and a superfamily II helicase. These are the …
Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix D: Gene Names N003l Through N847r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten
Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix D: Gene Names N003l Through N847r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten
Nebraska Center for Virology: Faculty Publications
Appendix D: Gene Names N003L through N847R
Document, in spreadsheet format, shows Gene Name, Genome Position, A.A. length, Peptid e Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.
Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix C: Gene Names C001r – C814l, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, William C. Nierman, James L. Van Etten
Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix C: Gene Names C001r – C814l, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, William C. Nierman, James L. Van Etten
Nebraska Center for Virology: Faculty Publications
Appendix C: Gene Names c001R – c814L
Document shows Gene Name, Genome Position, A.A. length, Peptide Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.
Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix B: Gene Names B001l – B886r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, William C. Nierman, James L. Van Etten
Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix B: Gene Names B001l – B886r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, William C. Nierman, James L. Van Etten
Nebraska Center for Virology: Faculty Publications
Appendix B: Gene Names B001L – B886R
Document shows Gene Name, Genome Position, A.A. length, Peptide Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.
Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix D: Gene Names C006r – C815l, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, Willaim C. Nierman, James L. Van Etten
Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix D: Gene Names C006r – C815l, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, Willaim C. Nierman, James L. Van Etten
Nebraska Center for Virology: Faculty Publications
Appendix D: Gene Names C006R – C815L
Document shows Gene Name, Genome Position, A.A. length, Peptide Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.
The Cost Of Reproduction: The Devil In The Details, Lawrence G. Harshman, Anthony J. Zera
The Cost Of Reproduction: The Devil In The Details, Lawrence G. Harshman, Anthony J. Zera
Anthony Zera Publications
The cost of reproduction is of fundamental importance in life-history evolution. However, our understanding of its mechanistic basis has been limited by a lack of detailed functional information at all biological levels. Here, we identify, evaluate and integrate recent studies in five areas examining the proximate mechanisms underlying the cost of reproduction. Rather than being alternate explanations, hormonal regulation and intermediary metabolism act in concert and have an overarching influence in shaping the cost of reproduction. Immune function is compromised by reproduction, as is resistance to environmental stress. These studies not only provide new information about mechanisms that comprise “the …
Effects Of Turnip Crinkle Virus Infection On The Structure And Function Of Mitochondria And Expression Of Stress Proteins In Turnips, James A. Blake, Kit W. Lee, Thomas Jack Morris, Thomas Elthon
Effects Of Turnip Crinkle Virus Infection On The Structure And Function Of Mitochondria And Expression Of Stress Proteins In Turnips, James A. Blake, Kit W. Lee, Thomas Jack Morris, Thomas Elthon
Nebraska Center for Virology: Faculty Publications
We have investigated the effect of turnip crinkle virus (TCV) infection on mitochondrial structure and function in turnips (Brassica rapa cultivar “Just Right”). TCV infection resulted in plants with small, mottled leaves with severely crinkled edges, and in a 46% reduction in storage root mass. TCV infection resulted in specific vesicularization of mitochondrial outer membranes where TCV replication is thought to occur, with no apparent affect on other cellular membrane systems. Immunoblot analysis of mitochondrial proteins from storage roots indicated that the TCV p28 protein, which is essential for viral replication, was associated with mitochondria and that mitochondrial heat …
Bird Movement Predicts Buggy Creek Virus Infection In Insect Vectors, Charles R. Brown, Mary Bomberger Brown, Amy T. Moore, Nicholas Komar
Bird Movement Predicts Buggy Creek Virus Infection In Insect Vectors, Charles R. Brown, Mary Bomberger Brown, Amy T. Moore, Nicholas Komar
School of Natural Resources: Faculty Publications
Predicting the spatial foci of zoonotic diseases is a major challenge for epidemiologists and disease ecologists. Migratory birds are often thought to be responsible for introducing some aviozoonotic pathogens such as West Nile and avian influenza viruses to a local area, but most information on how bird movement correlates with virus prevalence is anecdotal or indirect. We report that the prevalence of Buggy Creek virus (BCRV) infection in cimicid swallow bugs (Oeciacus vicarius), the principal invertebrate vector for this virus, was directly associated with the likelihood of movement by cliff swallows (Petrochelidon pyrrhonota), an amplifying host …
Ecological Correlates Of Buggy Creek Virus Infection In Oeciacus Vicarius, Southwestern Nebraska, 2004, Amy T. Moore, Eric A. Edwards, Mary Bomberger Brown, Nicholas Komar, Charles R. Brown
Ecological Correlates Of Buggy Creek Virus Infection In Oeciacus Vicarius, Southwestern Nebraska, 2004, Amy T. Moore, Eric A. Edwards, Mary Bomberger Brown, Nicholas Komar, Charles R. Brown
School of Natural Resources: Faculty Publications
Buggy Creek virus (family Togaviridae, genus Alphavirus, BCRV) is an alphavirus within the western equine encephalitis virus complex whose primary vector is the swallow bug, Oeciacus vicarius Horvath (Hemiptera: Cimicidae), an ectoparasite of the colonially nesting cliff swallow, Petrochelidon pyrrhonota, that is also a frequent host for the virus.We investigated ecological correlates of BCRV infection in 100-bug pools at 14 different swallow colony sites in southwestern Nebraska from summer 2004, by using plaque assay on Vero cells to identify cytopathic virus and reverse transcription-polymerase chain reaction to identify noncytopathic viral RNA. We found 26.7% of swallow bug pools …