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Articles 1 - 23 of 23
Full-Text Articles in Life Sciences
Interferon Regulatory Factor 7 Is Associated With Epstein-Barr Virus-Transformed Central Nervous System Lymphoma And Has Oncogenic Properties, Luwen Zhang, Jun Zhang, Que Lambert, Channing J. Der, Luis Del Valle, Judith Miklossy, Kamel Kahlili, You Zhou, Joseph S. Pagano
Interferon Regulatory Factor 7 Is Associated With Epstein-Barr Virus-Transformed Central Nervous System Lymphoma And Has Oncogenic Properties, Luwen Zhang, Jun Zhang, Que Lambert, Channing J. Der, Luis Del Valle, Judith Miklossy, Kamel Kahlili, You Zhou, Joseph S. Pagano
Nebraska Center for Virology: Faculty Publications
EBV transforms primary B cells, and the major EBV oncoprotein, latent membrane protein 1 (LMP-1), is required for the process. LMP-1 both induces the expression of IRF-7 and activates the IRF-7 protein by phosphorylation and nuclear translocation. Here we report that the expression of IRF-7 is increased in EBV-immortalized B lymphocytes compared with that in primary B cells. IRF-7 was phosphorylated and predominantly localized in the nucleus in the immortalized cells. The expression of IRF-7 was detected in 19 of 27 specimens of primary lymphomas of the human central nervous system by immunohistochemical analysis. The association between LMP-1 and IRF-7 …
Infectious Molecular Clone Of A Recently Transmitted Pediatric Human Immunodeficiency Virus Clade C Isolate From Africa: Evidence Of Intraclade Recombination, Ricky D. Grisson, Agnes-Laurence Chenine, Lan-Yu Yeh, Jun He, Charles Wood, Ganapati J. Bhat, Weidong Zu, Chipepo Kankasa, Ruth M. Ruprecht
Infectious Molecular Clone Of A Recently Transmitted Pediatric Human Immunodeficiency Virus Clade C Isolate From Africa: Evidence Of Intraclade Recombination, Ricky D. Grisson, Agnes-Laurence Chenine, Lan-Yu Yeh, Jun He, Charles Wood, Ganapati J. Bhat, Weidong Zu, Chipepo Kankasa, Ruth M. Ruprecht
Nebraska Center for Virology: Faculty Publications
Although human immunodeficiency virus type 1 (HIV-1) clade C continues to dominate the pandemic, only two infectious clade C proviral DNA clones have been described (N. Mochizuki, N. Otsuka, K. Matsuo, T. Shiino, A. Kojima, T. Kurata, K. Sakai, N. Yamamoto, S. Isomura, T. N. Dhole, Y. Takebe, M. Matsuda, and M. Tatsumi, AIDS Res. Hum. Retrovir. 15:1321–1324, 1999; T. Ndung’u, B. Renjifo, and M. Essex, J. Virol. 75:4964–4972, 2001). We have generated an infectious molecular clone of a pediatric clade C strain, HIV1084i, which was isolated from a Zambian infant infected either intrapartum or through breastfeeding. HIV1084i is an …
The Latent Membrane Protein 1 Of Epstein-Barr Virus Establishes An Antiviral State Via Induction Of Interferon-Stimulated Genes, Jun Zhang, Subash C. Das, Catherine Kotalik, Asit K. Pattnaik, Luwen Zhang
The Latent Membrane Protein 1 Of Epstein-Barr Virus Establishes An Antiviral State Via Induction Of Interferon-Stimulated Genes, Jun Zhang, Subash C. Das, Catherine Kotalik, Asit K. Pattnaik, Luwen Zhang
Nebraska Center for Virology: Faculty Publications
Epstein-Barr virus (EBV) infection is associated with several human cancers. Latent membrane protein 1 (LMP-1) is one of the key viral proteins required for transformation of primary B cells in vitro and establishment of EBV latency. In this report, we show that LMP-1 is able to induce the expression of several interferon (IFN)-stimulated genes (ISGs) with antiviral properties such as 2’-5’ oligoadenylate synthetase (OAS), stimulated trans-acting factor of 50 kDa (STAF- 50), and ISG-15. LMP-1 inhibits vesicular stomatitis virus (VSV) replication at low multiplicity of infection (0.1 pfu/cell). The antiviral effect of LMP-1 is associated with the ability of LMP-1 …
A Morph-Specific Daily Cycle In The Rate Of Jh Biosynthesis Underlies A Morph-Specific Daily Cycle In The Hemolymph Jh Titer In A Wing-Polymorphic Cricket, Zhangwu Zhao, Anthony J. Zera
A Morph-Specific Daily Cycle In The Rate Of Jh Biosynthesis Underlies A Morph-Specific Daily Cycle In The Hemolymph Jh Titer In A Wing-Polymorphic Cricket, Zhangwu Zhao, Anthony J. Zera
Anthony Zera Publications
A previous study documented a high amplitude, morph-specific daily cycle in the hemolymph JH titer in the wing-polymorphic cricket, Gryllus firmus. The JH titer rose and fell 10–20 fold in the flight-capable [LW(f), long-winged] morph during the late-photophase- early scotophase, while it was relatively constant during that time in the flightless (SW, short-winged) morph. In the present study we documented a dramatic morph-specific daily cycle in the in vitro rate of juvenile hormone (JH) biosynthesis that was tightly correlated with the hemolymph JH titer on days 5–7 of adulthood. Biosynthetic rates rose and fell 1–2 fold between the late …
Detection And Quantitation Of Bovine Respiratory Syncytial Virus Using Real-Time Quantitative Rt-Pcr And Quantitative Competitive Rt-Pcr Assays, Jenna Achenbaum, Christina L. Topliff, Ventzislav B. Vassilev, Ruben O. Donis, Kent M. Eskridge, Clayton L. Kelling
Detection And Quantitation Of Bovine Respiratory Syncytial Virus Using Real-Time Quantitative Rt-Pcr And Quantitative Competitive Rt-Pcr Assays, Jenna Achenbaum, Christina L. Topliff, Ventzislav B. Vassilev, Ruben O. Donis, Kent M. Eskridge, Clayton L. Kelling
Nebraska Center for Virology: Faculty Publications
A single tube, fluorogenic probe-based, real-time quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) assay was developed for detection and quantitation of bovine respiratory syncytial virus (BRSV) using BioRad’s iCycler iQ™. Real-time Q-RT-PCR was compared with quantitative competitive RT-PCR (QC-RT-PCR) and viral titers. Viral mRNA levels were measured in BRSV-infected bovine turbinate cell lysate harvested at eight time points (1.5, 6, 12, 24, 36, 48, 60, 72 h) post-infection. A homologous BRSV cRNA standard was used for quantitation of the mRNA by plotting a standard curve of cycle threshold (Ct) values versus standard 10-fold dilutions of cRNA of known concentrations. Detection as …
Cd8+ T Cells Responding To Influenza Infection Reach And Persist At Higher Numbers Than Cd4+ T Cells Independently Of Precursor Frequency, Timothy J. Powell, Deborah M. Brown, Joseph A. Hollenbaugh, Tina Charbonneau, Roslyn A. Kemp, Susan L. Swain, Richard Dutton
Cd8+ T Cells Responding To Influenza Infection Reach And Persist At Higher Numbers Than Cd4+ T Cells Independently Of Precursor Frequency, Timothy J. Powell, Deborah M. Brown, Joseph A. Hollenbaugh, Tina Charbonneau, Roslyn A. Kemp, Susan L. Swain, Richard Dutton
Nebraska Center for Virology: Faculty Publications
The activation, localization, phenotypic changes, and function of CFSE-labeled naive influenza-specific CD8+ and CD4+ T cells following influenza infection were examined. Response of adoptively transferred CD8+ T cells was seen earliest in draining lymph node. Highly activated cells were found later in the lung, airways, and spleen, were cytolytic, and expressed IFN-γ upon restimulation. Similar amounts of division at early time points, but higher numbers of CD8+ T cells, were detected at 9 and 30 days postinfection after cotransfer of CD4+ and CD8+ T cells followed by infection. Transfer of much smaller numbers of …
Preserved Antigenicity Of Hiv-1 P24 Produced And Purified In High Yields From Plants Inoculated With A Tobacco Mosaic Virus (Tmv)-Derived Vector, D. M. Pérez-Filgueira, B. P. Brayfield, S. Phiri, M. V. Borca, Charles Wood, Thomas Jack Morris
Preserved Antigenicity Of Hiv-1 P24 Produced And Purified In High Yields From Plants Inoculated With A Tobacco Mosaic Virus (Tmv)-Derived Vector, D. M. Pérez-Filgueira, B. P. Brayfield, S. Phiri, M. V. Borca, Charles Wood, Thomas Jack Morris
Papers in Virology
Production of structural proteins from foot-and-mouth disease virus (FMDV) and bovine herpes virus (BHV-1) in Nicotiana benthamiana through the use of a tobacco mosaic virus-based vector (TMV-30B) has been reported previously. The development of the TMV-30B-HISc vector, a new version that adds a C-terminal histidine (His) sequence to the foreign protein expressed is described. Coding sequences from the FMDV VPl protein and the core protein, p24, from a clade C HIV-1 isolate from Zambia were cloned into the new vector and infective RNAs were generated for each construct to inoculate N. benthamiana plants. His-tagged proteins were purified from inoculated leaves …
In Vitro Expression Of Full-Length And Truncated Bovine Respiratory Syncytial Virus G Proteins And Their Antibody Responses In Balb/C Mice, Ryan P. Brady, Christina L. Topliff, Clayton L. Kelling
In Vitro Expression Of Full-Length And Truncated Bovine Respiratory Syncytial Virus G Proteins And Their Antibody Responses In Balb/C Mice, Ryan P. Brady, Christina L. Topliff, Clayton L. Kelling
Nebraska Center for Virology: Faculty Publications
Bovine respiratory syncytial virus (BRSV) is a primary cause of lower respiratory tract disease in calves. Protection is incomplete following vaccination or natural infection, as re-infections are common. The objectives of this study were to create plasmid DNA constructs encoding the full-length, secreted, or conserved region of the BRSV G glycoprotein, and to compare and evaluate their expression in cell culture and potential to induce antibody responses in BALB/c mice. Transfection of COS-7 cells with plasmid DNA resulted in expression of the BRSV G region from each of the plasmid DNA constructs. Following inoculation of BALB/c mice with plasmid DNA, …
Effect Of A Juvenile Hormone Analogue On Lipid Metabolism In A Wing-Polymorphic Cricket: Implications For The Endocrine-Biochemical Bases Of Life-History Trade-Offs, Anthony J. Zera, Zhangwu Zhao
Effect Of A Juvenile Hormone Analogue On Lipid Metabolism In A Wing-Polymorphic Cricket: Implications For The Endocrine-Biochemical Bases Of Life-History Trade-Offs, Anthony J. Zera, Zhangwu Zhao
Anthony Zera Publications
The wing-polymorphic cricket, Gryllus firmus, has a flight-capable morph (LW[f]: long winged with functional flight muscles) and a flightless morph (SW: short winged with reduced nonfunctional flight muscles) that differ genetically in many aspects of lipid metabolism. To determine whether these differences result from genetically based alterations in endocrine regulation, the juvenile hormone mimic, methoprene, was applied to the LW(f) morph. This hormone manipulation converted the LW(f) morph into a SW phenocopy with respect to all aspects of lipid metabolism studied; that is, methoprene application decreased in vivo biosynthesis of total lipid and triglyceride, increased absolute and relative biosynthesis of …
Heat Shock Protein 70 Protects Cells From Cell Cycle Arrest And Apoptosis Induced By Human Immunodeficiency Virus Type 1 Viral Protein R, Yuqi Zhao, Larisa Dubrovsky, Tatiana Iordanskaya, Mongzhong Chen, Dong Liang, Michael Bukrinsky, Sergey Iordanskiy
Heat Shock Protein 70 Protects Cells From Cell Cycle Arrest And Apoptosis Induced By Human Immunodeficiency Virus Type 1 Viral Protein R, Yuqi Zhao, Larisa Dubrovsky, Tatiana Iordanskaya, Mongzhong Chen, Dong Liang, Michael Bukrinsky, Sergey Iordanskiy
Nebraska Center for Virology: Faculty Publications
Viral protein R (Vpr) of human immunodeficiency virus type 1 (HIV-1) is an accessory protein that plays an important role in viral pathogenesis. This pathogenic activity of Vpr is related in part to its capacity to induce cell cycle G2 arrest and apoptosis of target T cells. A screening for multicopy suppressors of these Vpr activities in fission yeast identified heat shock protein 70 (Hsp70) as a suppressor of Vpr-induced cell cycle arrest. Hsp70 is a member of a family of molecular chaperones involved in innate immunity and protection from environmental stress. In this report, we demonstrate that HIV-1 …
Defined Anaerobic Growth Medium For Studying Candida Albicans Basic Biology And Resistance To Eight Antifungal Drugs, Raluca Dumitru, Jacob M. Hornby, Kenneth W. Nickerson
Defined Anaerobic Growth Medium For Studying Candida Albicans Basic Biology And Resistance To Eight Antifungal Drugs, Raluca Dumitru, Jacob M. Hornby, Kenneth W. Nickerson
Papers in Microbiology
The polymorphic fungus Candida albicans is one of the most versatile opportunistic pathogens in humans. Many organs of the human body are potential targets for infection by this pathogen, but infection is commonly localized in the gastrointestinal tract, an environment providing anaerobic growth conditions. We describe a chemically defined anaerobic growth medium for four strains of Candida albicans (A72, SC5314, MEN, and 10261). It is a defined liquid glucose-phosphate-proline growth medium supplemented with oleic acid, nicotinic acid, and ammonium chloride. The cells did not require or respond to added ergosterol. Oleic acid and nicotinic acid are growth factors which are …
Prion Infection Of Skeletal Muscle Cells And Papillae In The Tongue, Ellyn R. Mulcahy, Jason C. Bartz, Anthony E. Kincaid, Richard Bessen
Prion Infection Of Skeletal Muscle Cells And Papillae In The Tongue, Ellyn R. Mulcahy, Jason C. Bartz, Anthony E. Kincaid, Richard Bessen
Nebraska Center for Virology: Faculty Publications
The presence of the prion agent in skeletal muscle is thought to be due to the infection of nerve fibers located within the muscle. We report here that the pathological isoform of the prion protein, PrPSc, accumulates within skeletal muscle cells, in addition to axons, in the tongue of hamsters following intralingual and intracerebral inoculation of the HY strain of the transmissible mink encephalopathy agent. Localization of PrPSc to the neuromuscular junction suggests that this synapse is a site for prion agent spread between motor axon terminals and muscle cells. Following intracerebral inoculation, the majority of PrP …
Enhanced Production Of Farnesol By Candida Albicans Treated With Four Azoles, Jacob M. Hornby, Kenneth Nickerson
Enhanced Production Of Farnesol By Candida Albicans Treated With Four Azoles, Jacob M. Hornby, Kenneth Nickerson
Papers in Microbiology
The dimorphic fungus Candida albicans excretes farnesol, which is produced enzymatically from the sterol biosynthetic intermediate farnesyl pyrophosphate. Inhibition of C. albicans by four azole antifungals, fluconazole, ketoconazole, miconazole, and clotrimazole, caused elevated farnesol production (10- to 45-fold). Furthermore, farnesol production occurs in both laboratory strains and clinical isolates (J. M. Hornby et al., Appl. Environ. Microbiol. 67:2982-2992, 2001) of C. albicans.
Distribution Of Kaposi Sarcoma–Associated Herpesvirus/Human Herpesvirus 8 In Maternal Saliva And Breast Milk In Zambia: Implications For Transmission, Brad P. Brayfield, Chipepo Kankasa, John T. West, Jubra Muyanga, Ganapati Bhat, Winslow Klaskala, Charles D. Mitchell, Charles Wood
Distribution Of Kaposi Sarcoma–Associated Herpesvirus/Human Herpesvirus 8 In Maternal Saliva And Breast Milk In Zambia: Implications For Transmission, Brad P. Brayfield, Chipepo Kankasa, John T. West, Jubra Muyanga, Ganapati Bhat, Winslow Klaskala, Charles D. Mitchell, Charles Wood
Nebraska Center for Virology: Faculty Publications
Background. The seroprevalence of Kaposi sarcoma–associated herpesvirus (KSHV)/human herpesvirus 8 (HHV-8) in sub-Saharan Africa suggests that multiple routes of transmission exist. In the present study,we examined 2 possible routes of mother-to-child transmission, through breast milk and saliva, during the first 6 months after delivery. Methods. The prevalence of HHV-8 DNA in the breast-milk cells (n=75), milk supernatant (n=56), colostrum (n=2), and saliva cells (n=65) of HHV-8–seropositive mothers who recently gave birth was examined. Polymerase chain reaction analysis was performed for the detection of HHV-8 in cross-sectional samples isolated at 2, 4, and 6 months after delivery.
Results. None of the …
Cd4 T Cell Responses To Influenza Infection, Deborah M. Brown, Eulogia Roman, Susan L. Swain
Cd4 T Cell Responses To Influenza Infection, Deborah M. Brown, Eulogia Roman, Susan L. Swain
Nebraska Center for Virology: Faculty Publications
Immune responses to viral infections involve a complex orchestration between innate signals and adaptive responses of specific T and B cells. Anti-viral CD4 cells can direct CD8 responses by secreting a Type 1 panel of cytokines including IFN-γ, IL-2 and TNF- α and can drive B cell production of IgG2a to neutralize infective viral particles. This review will focus specifically on the role of CD4 cells in the immune response to influenza, an acute, localized respiratory viral infection. We suggest that CD4 cells act as direct effectors in protection against influenza, may contribute to immunopathology and generate functionally distinct memory …
Multiple Signal Transducers And Activators Of Transcription Are Induced By Ebv Lmp-1, Luwen Zhang, Ke Hong, Jun Zhang, Joseph S. Pagano
Multiple Signal Transducers And Activators Of Transcription Are Induced By Ebv Lmp-1, Luwen Zhang, Ke Hong, Jun Zhang, Joseph S. Pagano
Nebraska Center for Virology: Faculty Publications
Epstein-Barr virus (EBV) latent membrane protein 1 (LMP-1) is required for EBV immortalization of primary B cells in vitro. Signal transducers and activators of transcription (STATs) play a pivotal role in the initiation and maintenance of certain cancers. STAT proteins, especially STAT-1, -3, and -5, are persistently tyrosine phosphorylated or activated in many cancers. We show here that EBV-infected type III latency cells, in which the EBV oncoprotein, LMP-1 is expressed, express high levels of four STATs (STAT-1, -2, -3, and -5A) and that LMP-1 is responsible for the induction of three (STAT-1, -2, and -3). In addition, the C-terminal …
Inoculum Size Effect In Dimorphic Fungi: Extracellular Control Of Yeast-Mycelium Dimorphism In Ceratocystis Ulmi, Jacob M. Hornby, Sarah M. Jacobitz-Kizzier, Donna J. Mcneel, Ellen C. Jensen, David S. Treves, Kenneth W. Nickerson
Inoculum Size Effect In Dimorphic Fungi: Extracellular Control Of Yeast-Mycelium Dimorphism In Ceratocystis Ulmi, Jacob M. Hornby, Sarah M. Jacobitz-Kizzier, Donna J. Mcneel, Ellen C. Jensen, David S. Treves, Kenneth W. Nickerson
Papers in Microbiology
We studied the inoculum size effect in Ceratocystis ulmi, the dimorphic fungus that causes Dutch elm disease. In a defined glucose-proline-salts medium, cells develop as budding yeasts when inoculated at >106 spores per ml and as mycelia when inoculated at type, age of the spores, temperature, pH, oxygen availability, trace metals, sulfur source, phosphorous source, or the concentration of glucose or proline. Similarly, it was not influenced by added adenosine, reducing agents, methyl donors, amino sugars, fatty acids, or carbon dioxide. Instead, growing cells excreted an unknown quorum-sensing factor that caused a morphological shift from mycelia to budding yeasts. …
Phylogenetic Analyses Of Texas Isolates Indicate An Evolving Subtype Of The Clade B Feline Immunodeficiency Viruses, Eric A. Weaver, Ellen W. Collisson, Margaret Slater, Guan Zhu
Phylogenetic Analyses Of Texas Isolates Indicate An Evolving Subtype Of The Clade B Feline Immunodeficiency Viruses, Eric A. Weaver, Ellen W. Collisson, Margaret Slater, Guan Zhu
Nebraska Center for Virology: Faculty Publications
Rigorous phylogenetic analyses were used to compare the nucleotide sequences of feline immunodeficiency virus strains isolated from Texas and throughout the world. The envelope V3-V4 sequences and capsid gene of the Texas isolates formed a cluster between subtypes B and E. Statistical comparisons with other published sequences confirmed that the Texas group is a unique cluster, possibly a new subtype, arising from subtype B.
Feline immunodeficiency virus (FIV) was initially isolated in 1987 from a cat in California with severe immunodeficiency and has been recognized as a common worldwide feline pathogen (11, 14, 19, 20, 33). FIV-infected cats exhibit a …
High Phosphate (Up To 600 Mm) Induces Pseudohyphal Development In Five Wild Type Candida Albicans, Jacob M. Hornby, Raluca Dumitru, Kenneth Nickerson
High Phosphate (Up To 600 Mm) Induces Pseudohyphal Development In Five Wild Type Candida Albicans, Jacob M. Hornby, Raluca Dumitru, Kenneth Nickerson
Kenneth Nickerson Papers
A method is described for the formation of nearly 100% pseudohyphae populations of wild-type Candida albicans A72. The method employs fungal growth at 37 °C (ca. 5×106 cells/ml) in a glucose–proline–N-acetyl-glucosamine medium supplemented with up to 600 mM phosphate (KH2PO4/K2HPO4 1:1) at pH 6.5. Four other strains of C. albicans (MEN, 10261, SG5314 and CAI-4) also formed pseudohyphae under these conditions, although the phosphate response profiles differed in the concentration required for each strain to form pseudohyphae.
The Hemolymph Jh Titer Exhibits A Large-Amplitude, Morph-Dependent, Diurnal Cycle In The Wing-Polymorphic Cricket, Gryllus Firmus, Zhangwu Zhao, Anthony J. Zera
The Hemolymph Jh Titer Exhibits A Large-Amplitude, Morph-Dependent, Diurnal Cycle In The Wing-Polymorphic Cricket, Gryllus Firmus, Zhangwu Zhao, Anthony J. Zera
Anthony Zera Publications
The hemolymph juvenile hormone (JH) titer was measured in over 500 flight-capable and flightless, adult female Gryllus firmus at 3–6 h intervals during each of days 2–8 of adulthood. The flight-capable morph exhibited a large-amplitude daily cycle in the hemolymph JH titer, while the flightless morph exhibited a barely perceptible cycle. The JH titer cycle was observed on all days in the flight-capable morph, but the large amplitude cycle (>15–20 fold increase in mean titer; >100-fold increase in some individuals), began on day 5. For both the large and small amplitude cycles, the JH titer peaked near the end …
Cd46-Mediated Transduction Of A Species D Adenovirus Vaccine Improves Mucosal Vaccine Efficacy, Zenaido T. Camacho, Mallory A. Turner, Michael A. Barry, Eric A. Weaver
Cd46-Mediated Transduction Of A Species D Adenovirus Vaccine Improves Mucosal Vaccine Efficacy, Zenaido T. Camacho, Mallory A. Turner, Michael A. Barry, Eric A. Weaver
Nebraska Center for Virology: Faculty Publications
The high levels of preexisting immunity against Adenovirus type 5 (Ad5) have deemed Ad5 unusable for translation as a human vaccine vector. Low seroprevalent alternative viral vectors may be less impacted by preexisting immunity, but they may also have significantly different phenotypes from that of Ad5. In this study we compare species D Ads (26, 28, and 48) to the species C Ad5. In vitro transduction studies show striking differences between the species C and D viruses. Most notably, Ad26 transduced human dendritic cells much more effectively than Ad5. In vivo imaging studies showed strikingly different transgene expression profiles. The …
Phylogenetic Analyses Of Texas Isolates Indicate An Evolving Subtype Of The Clade B Feline Immunodeficiency Viruses, Eric A. Weaver, Ellen W. Collisson, Margaret Slater, Guan Zhu
Phylogenetic Analyses Of Texas Isolates Indicate An Evolving Subtype Of The Clade B Feline Immunodeficiency Viruses, Eric A. Weaver, Ellen W. Collisson, Margaret Slater, Guan Zhu
Nebraska Center for Virology: Faculty Publications
Rigorous phylogenetic analyses were used to compare the nucleotide sequences of feline immunodeficiency virus strains isolated from Texas and throughout the world. The envelope V3-V4 sequences and capsid gene of the Texas isolates formed a cluster between subtypes B and E. Statistical comparisons with other published sequences confirmed that the Texas group is a unique cluster, possibly a new subtype, arising from subtype B.
Genomes Of The Parapoxviruses Orf Virus And Bovine Papular Stomatitis Virus, Gustavo A. Delhon, E. R. Tuhlman, C. L. Afonso, Z. Lu, A. De La Concha-Bermejillo, H. D. Lehmkuhl, M. E. Piccone, G. F. Kutish, D. L. Rock
Genomes Of The Parapoxviruses Orf Virus And Bovine Papular Stomatitis Virus, Gustavo A. Delhon, E. R. Tuhlman, C. L. Afonso, Z. Lu, A. De La Concha-Bermejillo, H. D. Lehmkuhl, M. E. Piccone, G. F. Kutish, D. L. Rock
Nebraska Center for Virology: Faculty Publications
Bovine papular stomatitis virus (BPSV) and orf virus (ORFV), members of the genus Parapoxvirus of the Poxviridae, are etiologic agents of worldwide diseases affecting cattle and small ruminants, respectively. Here we report the genomic sequences and comparative analysis of BPSV strain BV-AR02 and ORFV strains OV-SA00, isolated from a goat, and OV-IA82, isolated from a sheep. Parapoxvirus (PPV) BV-AR02, OV-SA00, and OV-IA82 genomes range in size from 134 to 139 kbp, with an average nucleotide composition of 64% G+C. BPSV and ORFV genomes contain 131 and 130 putative genes, respectively, and share colinearity over 127 genes, 88 of which …