Life Sciences Commons^™

Determining The Impacts Of Corn Silage Mixing Ratios On Hydrogen Production From Beef Cattle Manure, Katya Faber-Quimby

Honors Theses

With global warming becoming an increasingly serious threat and a growing population requiring additional energy resources, interest in the production of clean energy from the agricultural sector has surged in popularity. Biohydrogen, also known as green hydrogen, is H₂ that has been produced using renewable energy, such as from agricultural byproducts or waste materials. It presents a promising alternative to nonrenewable resources due to its high potential for energy storage and lack of carbon emissions when used. Cattle manure has been explored as a resource for the production of biohydrogen. Selecting for hydrogen-producing microbes in the manure serves the …

Predicted Structure And Analysis Of The Glycoprotein From Sars-Cov-2 Variants Of Interest, Boris Shabaltiy

Honors Theses

SARS-CoV-2 has had a devastating effect on the world, and while the public concern and severity of the diseased caused by it have decreased, it is still crucial to monitor the virus for mutations so we can rapidly identify new variants of concern, and then rapidly prepare new vaccines and treatments. The SARS-CoV-2 spike (S) protein from the first isolates of the virus (root variant) was structurally characterized in early 2021. The spike protein structure, designated 7CZW, was uploaded to RCSB Protein Data Bank (RCSB PDB). All subsequent mutations the S protein has accumulated are based on the amino acid …

Antimicrobial Resistance In Eskape Pathogens And Its Effect On Modern Medicine And Treatment, Cameran Runge

Honors Theses

Abstract

ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella

pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter

spp.) are seeing a growing resistance to multiple classes of antibiotics. Misuse and overuse of antibiotics have played directly into the resistance observed, and the problem is growing exponentially. Antibiotic resistance is partially due to several intrinsic factors limiting the drug's uptake. These include efflux pumps, increased biofilm production, and reduced cell wall permeability in the resistant bacteria. ESKAPE pathogens also acquire resistance through horizontal gene transfer and plasmids. As antibiotics have become less effective, the bacteria can continue to thrive, leading to a detrimental …

Syndemics And Social Factors: Infectious Disease Patterns Within The Population Of People Experiencing Homelessness In The United States, Kathleen Berzonsky

Honors Theses

Individuals experiencing homelessness are at increased risk of suffering from infectious diseases. This is due to a number of social factors and healthcare disparities, as well as the idea of syndemics, by which diseases cluster together to worsen disease burden. Current intervention strategies approach treatment from a post-infection perspective, but reducing transmission rates of infectious diseases within the population of people experiencing homelessness will require a shift in the healthcare framework. The issue of people experiencing homelessness must be viewed through a biosocial lens, focusing on preventative care and treatment. I provide an overview of the social factors governing infectious …

Tiny Earth, Tinier Microbes: An Experiential Learning Approach To Antibiotic Discovery, Emily Kassing

Honors Theses

Antimicrobial resistance is one of the greatest global health challenges of the 21^st century as antibiotic discovery has slowed even as scientific knowledge about AMR has progressed. At the same time, science education has turned to active learning approaches like CUREs, or course-based undergraduate research experiences, to achieve educational objectives while engaging students in real-life research. The Tiny Earth Project is a global research initiative that seeks to crowdsource antibiotic discovery by recruiting undergraduate students to screen soil samples for antibiotic producers. The goal of this study was to determine the viability of translating the Tiny Earth programming to …

Determining Pairwise Interactions To Predict Species Interactions In A Complex Community Of Gut Bacteria., Makenzie Maroney

Honors Theses

The human gastrointestinal (GI) tract houses a diverse and expansive microbial community known as the microbiota. The relationship between the host and the microbiota is considered to be mutualistic, as trillions of bacteria that reside in the hospitable GI tract can assist in essential host functions, including contributing to metabolism and immunity against disease. An important subpopulation of the GI microbiota is the mucin-associated biofilm that is comprised of a dense layer of bacteria that forms on the GI mucin layer. The mucin layer provides an alternative niche to the GI lumen; the glycans of the mucin and the subsequent …

Genomic Analysis Of Acetoanaerobium Sp. Vlb-1, An Anaerobic Bacterium Isolated From Nebraska's Eastern Saline Wetlands, Claire Martin

Honors Theses

Through the analysis of genetic code, the genomic and physiological characteristics of an environmental anaerobic bacterial isolate, Acetoanaerobium sp. strain VLB-1, can be determined and significant pathways can be identified in its significance to the environment. This particular organism was isolated from the Eastern Saline Wetlands in Lincoln, NE. With this isolate, an investigation into elemental and amino acid cycling via the Stickland reaction and the Wood-Ljungdahl pathway was conducted to determine possible metabolic products. The Stickland reaction is a relatively newly discovered pathway, observed in the genus Clostridium. A. sticklandii is the main model for this method of …

Genetic Diversity Of Potassium Ion Channel Proteins Encoded By Chloroviruses That Infect Chlorella Heliozoae, Carter Murry

Honors Theses

Chloroviruses are large, plaque-forming, double-stranded DNA viruses that infect chlorella-like green algae that live in a symbiotic relationship with protists. Chloroviruses have a genome from 290 to 370 kb, and they encode as many as 400 proteins. One interesting feature of chloroviruses is that they encode a potassium ion (K⁺) channel protein named Kcv. K⁺ channels are essential proteins for life. They cross the membrane of the cell to quickly and selectively allow K⁺ in or out of the cell. This helps regulate the electrical charge of a cell which in turn helps control electrical signaling …

A Comparative Analysis Of The Fermentation Capabilities Of Various Bifidobacterium Strains, Ella Oney

Honors Theses

Bifidobacterium is a genus of anaerobic bacteria that are commonly found to inhabit the gastrointestinal tract of many members of the animal kingdom. These microorganisms are adapted to obtain their carbon from the breakdown of complex carbohydrates. Marmosets, a mammal whose gut microbiome is inhabited by high levels of Bifidobacteria, consume gum Arabic as a major part of their diet. The purpose of this experiment is to determine whether Bifidobacterium strains isolated from the guts of marmosets are able to degrade and ferment this complex carbohydrate or one of its main constituents, arabinose. This was accomplished by inoculating isolates of …

Factors Affecting The Parasitism Of Gregarine Species In Grasshoppers In Western Nebraska, Alexandra Creigh, Kristin Mclarty, Scott Lyell Gardner

Honors Theses

We conducted a survey of the species of parasites found in grasshoppers around the area of Dunwoody’s Pond to investigate whether there were any species specific parasitic relationships occurring there. We collected grasshoppers from several areas around Dunwoody’s Pond totaling 15 species including Melanoplus bivittatus (Say 1825) , Melanoplus sanguinipes (Fabricius, 1798) , Melanoplus differentialis (Thomas 1865), and Melanoplus femurrubrum (De Geer 1773), along with others (Seymour et al., 2009) and compared the parasites found in them. This study is similar to one conducted in South America by Lloyd in 1951, and we hoped to find connections between species to …