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Full-Text Articles in Life Sciences
The Effects Of Temperature And Phasins On Polyhydroxyalkanoic Acid (Pha) Accumulation In Heterologous Hosts Containing A Pha Synthase From Vibrio Species B-18, Edward R. Lyons
Master's Theses, Dissertations, Graduate Research and Major Papers Overview
Polyhydroxyalkanoic acids (PHA) are bacterial carbon reserves with application as biodegradable biocompatible plastics. PHA synthase, encoded by phaC, creates the PHA polymer. Evidence presented by many researchers has emphasized the role of phasin proteins in PHA accumulation. Phasins have been implicated in PHA granule localization, PHA synthase activation, and possibly chaperone and nucleoid binding functions. The phaC gene from Vibrio species B-18 with and without an upstream gene showing homology to phasin genes (orf2) were placed into runaway replication vectors, which use temperature to control gene expression. The aim of this thesis is to explore earlier observations that lower temperatures …
A Tale Of Two Investigations In Molecular Biology: The Use Of Pcr Technology To Identify Bacteria Containing Genes For Pha Synthesis And The Antibiotic Sensitivity Profile Of Vibrio Species B-18, Diana B. Lizarazo
Honors Projects
The Vibrio species, B-18, produces PHA, a bacterial storage material with application as a biodegradable plastic. Phase one of this project aimed at determining if PCR technology could be used to find other bacteria that produce a similar type of PHA. Results showed that the primer sets utilized were not able to identify bacteria with PHA genes. Phase two hoped to discover if it would be possible to introduce Escherichia coli plasmids into B-18 using electroporation. Results demonstrated that electroporation was not useful for introducing plasmids into B-18.