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Altered Pathogenesis Of A Mutant Of The Murine Coronavirus Mhv-A59 Is Associated With A Q159l Amino Acid Substitution In The Spike Protein, Isabelle Leparc-Goffart, Susan T. Hingley, Ming Chua, Xinhe Jiang, Ehud Lavi, Susan R. Weiss
Altered Pathogenesis Of A Mutant Of The Murine Coronavirus Mhv-A59 Is Associated With A Q159l Amino Acid Substitution In The Spike Protein, Isabelle Leparc-Goffart, Susan T. Hingley, Ming Chua, Xinhe Jiang, Ehud Lavi, Susan R. Weiss
PCOM Scholarly Papers
C12, an attenuated, fusion delayed, very weakly hepatotropic mutant of mouse hepatitis virus strain A59 (MHV-A59) has been further characterized. We have previously shown that C12 has two amino acid substitutions relative to wild type virus in the spike protein, Q159L (within a region of S1 shown to bind to viral receptor in an in vitro assay) and H716D (in the proteolytic cleavage recognition site). We have sequenced the rest of the 31-kb genome of C12 and compared it to wild type virus. Only three additional amino acids substitutions were found, all encoded within the replicase gene. Analysis of C12 …