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Full-Text Articles in Life Sciences
Enzyme Linked Immunosorbent Assay For Anatid Herpesvirus, Margaret Anne Lewis
Enzyme Linked Immunosorbent Assay For Anatid Herpesvirus, Margaret Anne Lewis
Biological Sciences Theses & Dissertations
Duck plague virus causes a hemorrhagic disease affecting the family Anatidae, resulting in 60-80% mortality in captive flocks. Plaque and neutralization assays for Anatid herpesvirus (AHV) require 5-10 days for completion and are restricted by the seasonal availability of fertile duck eggs. The enzyme linked innnunosorbent assay (ELISA) is rapid and specific and avoids the use of living cells. The Holland strain of AHV was grown on duck embryo fibroblasts and purified by banding on CsCl gradients. The resulting virus was used to raise antisera in prebled New Zealand white male rabbits. Antibody production was confirmed in double diffusion plates …
Petroleum-Utilizing Bacteria: The Biological Line Defense Against Oil Pollution, Steven W. Sokolowski
Petroleum-Utilizing Bacteria: The Biological Line Defense Against Oil Pollution, Steven W. Sokolowski
Biological Sciences Theses & Dissertations
A silica gel-petroleum (SGP) medium was developed which is reliable, approximately the same cost as that of the corresponding agar-based petroleum medium, and can be made sufficiently firm to use in streaking or the spread-plate technique. Advantages of the medium include: the incorporation of all of the major ions of seawater in accordance with Dittmar's Law (the salinity of which can easily be varied from 0 to 35 parts per thousand); a wide workable pH range (5.5 to 11.6); negligable pH drift(~ 0.2 pH units after 166 days); the elimination of all but negligable amounts of syneresis, the ability to …