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Full-Text Articles in Life Sciences
Finding Clues To Phosphate Homeostasis Through Transposon Sequencing, Tanner Dean, William Mccleary
Finding Clues To Phosphate Homeostasis Through Transposon Sequencing, Tanner Dean, William Mccleary
Journal of Undergraduate Research
Though Escherichia Coli is a thoroughly investigated species of bacteria, questions still remain concerning genes involved in phosphate homeostasis. Phosphate homeostasis is the idea that a cell or bacterium adapts to changing environmental phosphate concentrations. Cells need to adapt to changing phosphate conditions because phosphate is essential to the biochemistry of many cellular processes. A cell therefore, must be able to collect and store phosphate when there is excess or scavenge and utilize stored phosphate in times when there are low levels of extracellular phosphate available. This process is not entirely understood in E.coli or other single celled organisms. A …
Creation Of An Eight-Member Plasmid Library For Promoter Swapping To Control Chromosomal Gene Expression In E. Coli, Matt Phillips, Dr. William Mccleary
Creation Of An Eight-Member Plasmid Library For Promoter Swapping To Control Chromosomal Gene Expression In E. Coli, Matt Phillips, Dr. William Mccleary
Journal of Undergraduate Research
The ability to control gene expression in bacteria has been essential in solving problems in many fields, including medicine and environmental protection. Recent advances in genomic and metabolic modeling tools have led to the development of a new technique called promoter swapping which enables researchers to “swap” any native gene promoter with one that has been specifically engineered. Promoter swapping uses viral recombination proteins to swap DNA in the chromosome rather than in plasmids, allowing for constant and permanent expression levels. Our goal was to create eight template plasmids with promoters of increasing strengths to be used in promoter swapping. …