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Full-Text Articles in Life Sciences
Quantification Of Interactions Between Influenza Hemagglutinin And Host Cell Phosphoinositides By Super-Resolution Microscopy, Matthew T. Parent
Quantification Of Interactions Between Influenza Hemagglutinin And Host Cell Phosphoinositides By Super-Resolution Microscopy, Matthew T. Parent
Electronic Theses and Dissertations
The influenza viral membrane protein hemagglutinin (HA) forms dense nanoscale clusters on host cell plasma membranes (PM), but the mechanisms that direct HA clustering are not well understood. Previous studies have observed HA associated with actin rich regions of the PM, but there are no known direct interactions between HA and actin. Phosphatidylinositol 4,5-biphosphate (PIP2) is a signaling lipid in the PM which can regulate the actin cytoskeleton, and actin comets initiated by PIP2 are known to be exploited by HA to reach the PM of infected cells. PIP2 is also used by other viruses, such as HIV and Ebola, …
Investigating High Speed Localization Microscopy Through Experimental Methods, Data Processing Methods, And Applications Of Localization Microscopy To Biological Questions, Andrew J. Nelson
Electronic Theses and Dissertations
Fluorescence Photoactivation Localization Microscopy(FPALM) and other super resolution localization microscopy techniques can resolve structures with nanoscale resolution. Unlike techniques of electron microscopy, they are also compatible with live cell and live animal studies, making FPALM and related techniques ideal for answering questions about the dynamic nature of molecular biology in living systems. Many processes in biology occur on rapid sub second time scales requiring the imaging technique to be capable of resolving these processes not just with a high enough spatial resolution, but with an appropriate temporal resolution. To that end, this Dissertation in part investigates high speed FPALM as …
The Role Of Caveolin In The Toll-Like Receptor (Tlr) Signaling Pathway, Richard H. Luc
The Role Of Caveolin In The Toll-Like Receptor (Tlr) Signaling Pathway, Richard H. Luc
Honors College
Upon viral infection, the host’s immune system can induce the antiviral state to protect from further infection. In this antiviral state, RNA synthesis and protein synthesis are downregulated, and viral replication is inhibited (Kawai and Akira, 2006). Two of the key pathways critical to establishing the antiviral state include the Toll-like receptor (TLR) and the interferon (IFN) receptor (IFNR) pathways (Kawai and Akira, 2006). The TLR pathway is critical to recognizing viral components, while the IFNR pathway is vital to activating genes necessary for inducing the antiviral state. As the host evolves to combat viral infections, viruses have also evolved …