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Full-Text Articles in Life Sciences
Electroporation Of Pediococcus Pentosaceus And The Curing Rate Of Plasmids, Heidi E. Shields
Electroporation Of Pediococcus Pentosaceus And The Curing Rate Of Plasmids, Heidi E. Shields
Undergraduate Honors Capstone Projects
Genetic engineering is a relatively new process and is a major focus of research in various fields, such as medicine, plant genetics, and food science. The potential applications of genetic engineering include designer drug production, mass protein production, and enhanced food processing. Finding an appropriate host organism to be used in genetic engineering is an important preliminary step. Pediococcus pentosaceus has been suggested as a bacterial species with such potential. Since plasmids are often the vector of choice in genetic recombination, this research tests the ability of P. pentosaceus to take up and incorporate plasmids into its genome. Two plasmids …
Regulation Of Cardiac Fibroblast Metalloprotease Secretion, Glen De Guzman
Regulation Of Cardiac Fibroblast Metalloprotease Secretion, Glen De Guzman
Undergraduate Honors Capstone Projects
Matrix metalloproteases (MMPs) are proteolytic enzymes secreted by cardiac fibroblasts. Altered expression of MMPs plays an important role in congestive heart failure and other cardiovascular diseases1,2. Emerging evidence has led us to consider the role of phosphatidylinositol 4-kinase (PtdIns 4-kinase) in regulating MMP secretion in cardiac fibroblast cells3-7. PtdIns 4-kinase is a lipid kinase involved in various cellular processes including protein secretion. This project was designed to test the hypothesis that PtdIns 4-kinase controls MMP secretion. Techniques such as cardiac cell culture, transfection, and in-gel zymography were used for this experiment. Results demonstrated that PtdIns 4-kinase …