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The Effect Of A Balanced Amino Acid Infusion On Hepatic Regeneration In Rats, Robert Arthur Anstadt Jan 1987

The Effect Of A Balanced Amino Acid Infusion On Hepatic Regeneration In Rats, Robert Arthur Anstadt

University of the Pacific Theses and Dissertations

The efficacy of a balanced amino acid infusion on recovery from temporary hepatic insufficiency and its effect on plasma ammonia levels in partially hepatectomized rats were studied.

Polyethylene implant buttons were developed to secure animals to the infusion apparatus. Rats under went jugular vein cannulation, and 70% partial hepatectomy. Test solutions were continuously infused (30 ml/d). Plasma samples were taken through the jugular vein cannula and analyzed for bilirubin concentration, ammonia concentration, LDH activity, and GPT activity.

LDH and bilirubin results were inconclusive. Results from GPT activity assays suggest that both Freamine III and the balanced formula provided injured hepatocytes …


High Pressure Liquid Chromatography Ion Exchange Studies On Bile Relating To The Postmortem Interval, Bertha Louise Martin Jan 1987

High Pressure Liquid Chromatography Ion Exchange Studies On Bile Relating To The Postmortem Interval, Bertha Louise Martin

University of the Pacific Theses and Dissertations

It is the purpose of this, then, to investigate the feasibility of using the changes of concentrations of small iconic decomposition products in bile as indicators of time since death. High pressure liquid chromatography was selected for measurement of these changes.