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Factors Affecting Sperm Motility Of Tetraploid Pacific Oysters, Qx Dong, B Eudeline, Standish K. Allen Jr., Tr Tiersch
Factors Affecting Sperm Motility Of Tetraploid Pacific Oysters, Qx Dong, B Eudeline, Standish K. Allen Jr., Tr Tiersch
VIMS Articles
Factors such as osmotic pressure, extender solution, addition of caffeine, and pH have been shown to affect sperm motility in aquatic species. We evaluated the effects of 18 osmotic pressures, two extender solutions, seven caffeine concentrations, and a pH range of 3 to 14 on motility of sperm from tetraploid Pacific oysters, Crassostrea gigas. Motility was highest at 1000 mOsmol/kg (mean +/- SD: 83 +/- 14%). Calcium-free Hanks' balanced salt solution yielded significantly higher sperm motility than did artificial seawater. Sperm motility increased with caffeine concentrations to 20 mM (81 +/- 12%) and decreased when concentrations were higher than 50 …