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Full-Text Articles in Life Sciences
Propagation And In Vitro Studies Of Perkinsus Marinus, Jerome F. Lapeyre
Propagation And In Vitro Studies Of Perkinsus Marinus, Jerome F. Lapeyre
VIMS Articles
The development of continuous cultures of Perkinsus marinus (Apicomplexa) is a major breakthrough that will lead to a better understanding of this deadly oyster pathogen. More than 10 P. marinus isolates are currently in continuous cultures. Culture media used to propagate P. marinus range from media designed for the culture of mammalian cells to protein-free chemically defined media. Continuous cultures of P. marinus can be initiated from a variety of infected oyster tissues or from isolated hypnospores (i.e., the enlarged stage of P. marinus from oyster tissue incubated in Ray's fluid thioglycollate medium). P. marinus cells adapt well to culture …
Epizootiology Of Perkinsus Marinus Disease Of Oysters In Chesapeake Bay, With Emphasis On Data Since 1985, Eugene Burreson, Lisa M. Ragone Calvo
Epizootiology Of Perkinsus Marinus Disease Of Oysters In Chesapeake Bay, With Emphasis On Data Since 1985, Eugene Burreson, Lisa M. Ragone Calvo
VIMS Articles
Since 1987 Perkinsus marinus has been the most important pathogen of the eastern oyster, Crassostrea virginica, in Chesapeake Bay because of its widespread distribution and persistence in low salinity areas. The pathogen became established on all oyster beds in the Chesapeake Bay as a result of natural spread during the consecutive drought years from 1985 to 1988 or by movement of infected oysters during the same period. Elevated salinities resulting from drought conditions and concomitant warm winters allowed P. marinus to proliferate in what were historically low salinity areas. Oyster mortality was high on most beds and landings of market …
A Comparison Of Crassostrea Gigas And Crassostrea Virginica: Effects Of Temperature And Salinity On Susceptibility To The Protozoan Parasite, Perkinsus Marinus, Fu-Lin C. Chu, Aswani Volety, G Constantin
A Comparison Of Crassostrea Gigas And Crassostrea Virginica: Effects Of Temperature And Salinity On Susceptibility To The Protozoan Parasite, Perkinsus Marinus, Fu-Lin C. Chu, Aswani Volety, G Constantin
VIMS Articles
The susceptibility of diploid and triploid (2N and 3N) Crassostrea gigas to Perkinsus marinus was compared, in the laboratory, with that of Crassostrea virginica al three test temperatures (10, 15, and 25 degrees C) at 20-22 ppt and at three test salinities (3, 10, and 20 ppt) at a temperature of 19-22 degrees C. Experimental oysters were challenged twice with freshly isolated P, marinus meronts, after acclimation to test temperatures and salinities. Although infection prevalence and intensity increased with temperature (p = 0.0001) and salinity in P. marinus-challenged oysters of both oyster species: they were highest in C. virginica groups. …
Foreword, Frank O. Perkins
History Of Perkinsus Marinus, A Pathogen Of Oysters In Chesapeake Bay 1950-1984, Jd Andrews
History Of Perkinsus Marinus, A Pathogen Of Oysters In Chesapeake Bay 1950-1984, Jd Andrews
VIMS Articles
The pathogen Perkinsus marinus (Dermo) was discovered in Chesapeake Bay in 1950. It was already widely distributed in the Bay and caused annual mortality below the mouth of the Rappahannock River. Annual mortality in trayed oysters at the Virginia Institute of Marine Science (VIMS) varied annually from 24% to 57% at this most favorable site for the disease. Over 2 million bushels of seed oysters from the James River public beds were transplanted annually to private beds in 4 major growing areas. These were Hampton Roads, lower Bay proper, Mobjack Bay at mouth of York River, and the Rappahannock River. …
Laboratory Investigations Of Susceptibility, Infectivity, And Transmission Of Perkinsus Marinus In Oysters, Fu-Lin C. Chu
Laboratory Investigations Of Susceptibility, Infectivity, And Transmission Of Perkinsus Marinus In Oysters, Fu-Lin C. Chu
VIMS Articles
The protozoan parasite, Perkinsus marinus (Dermo), has caused significant mortality in the eastern oyster, Crassostrea virginica, along the east coast of the United States and the Gulf of Mexico, since the 1950s. Because of its current expanded distribution and increased abundance, P. marinus is now considered more prevalent in the mid-Atlantic waters and the Chesapeake Bay in particular, than another protozoan pathogen, Haplosporidium nelsoni (MSX). The susceptibility, infectivity/pathogenicity, and transmission of P. marinus in eastern oysters were investigated in numerous laboratory studies, The influence of environmental factors such as temperature, salinity, and pollution on the interaction between the host oyster …
The Structure Of Perkinsus Marinus (Mackin, Owen And Collier, 1950) Levine, 1978 With Comments On Taxonomy And Phylogeny Of Perkinsus Spp., Frank O. Perkins
The Structure Of Perkinsus Marinus (Mackin, Owen And Collier, 1950) Levine, 1978 With Comments On Taxonomy And Phylogeny Of Perkinsus Spp., Frank O. Perkins
VIMS Articles
A description of the structure of the Crassostrea virginica pathogen Perkinsus marinus is provided from observations at the light and transmission electron microscope levels of detail and includes cellular multiplication (palintomy) in the host and zoosporulation in estuarine water as well as observations of cells in axenic culture. The description is primarily a review of previously published information; however, new information is provided on development of walled outgrowths from hypnospores derived from fluid thioglycollate medium treatment of infected host tissue. The protoplast within the outgrowths subdivides to yield small unicells which escape into the ambient water, or the protoplast emerges …