Life Sciences Commons^™

Bioinformatic And Experimental Approaches For Deeper Metaproteomic Characterization Of Complex Environmental Samples, Ramsunder Mahadevan Iyer

Doctoral Dissertations

The coupling of high performance multi-dimensional liquid chromatography and tandem mass spectrometry for characterization of microbial proteins from complex environmental samples has paved the way for a new era in scientific discovery. The field of metaproteomics, which is the study of protein suite of all the organisms in a biological system, has taken a tremendous leap with the introduction of high-throughput proteomics. However, with corresponding increase in sample complexity, novel challenges have been raised with respect to efficient peptide separation via chromatography and bioinformatic analysis of the resulting high throughput data. In this dissertation, various aspects of metaproteomic characterization, including …

Rare Occurrences Of Free-Living Bacteria Belonging To Sedimenticola From Subtidal Seagrass Beds Associated With The Lucinid Clam, Stewartia Floridana, Aaron M. Goemann

Masters Theses

Lucinid clams and their sulfur-oxidizing endosymbionts comprise two compartments of a three-stage, biogeochemical relationship among the clams, seagrasses, and microbial communities in marine sediments. A population of the lucinid clam, Stewartia floridana, was sampled from a subtidal seagrass bed at Bokeelia Island Seaport in Florida to test the hypotheses: (1) S. floridana, like other lucinids, are more abundant in seagrass beds than bare sediments; (2) S. floridana gill microbiomes are dominated by one bacterial operational taxonomic unit (OTU) at a sequence similarity threshold level of 97% (a common cutoff for species level taxonomy) from 16S rRNA genes; …

Environmental Controls On The Diversity And Distribution Of Endosymbionts Associated With Phacoides Pectinatus (Bivalvia: Lucinidae) From Shallow Mangrove And Seagrass Sediments, St. Lucie County, Florida, Thomas Walters Doty

Masters Theses

Lucinid bivalves are capable of colonizing traditionally inhospitable shallow marine sediments due to metabolic functions of bacterial endosymbionts located within their gills. Because lucinids can often be the dominant sediment infauna, defining their roles in sediment and pore fluid geochemical cycling is necessary to address concerns related to changes in coastal biological diversity and to understanding the sensitivity of threatened coastal ecosystems over time. However, there has been limited research done to understand the diversity and distribution of many lucinid chemosymbiotic systems. Therefore, the goals of this thesis were to evaluate the distribution of Phacoides pectinatus and its endosymbiont communities …

Elucidating The Impact Of Roseophage On Roseobacter Metabolism And Marine Nutrient Cycles, Nana Yaw Darko Ankrah

Doctoral Dissertations

As the most abundant biological entities in marine environments, viruses are an important component of marine food webs. The activity of viruses contributes significantly to the mortality of marine microorganisms, ultimately influencing biological function and chemical composition of aquatic systems by impacting species composition and flow of carbon, nitrogen and other nutrients. Despite the growing recognition that viral activity contributes to marine biogeochemical cycles, the extent to which virus infection reshapes host metabolism and the effect of this alteration on the composition of host lysate remains poorly understood. Additionally, the degree to which natural bacterioplankton communities metabolise the released lysate …

Fishes Of The Choctawhatchee River System In Southeastern Alabama And Northcentral Florida, Thomas P. Simon, Charles C. Morris, Bernard R. Kuhajda, Carter R. Gilbert, Henry L. Bart Jr., Nelson Rios, Paul M. Stewart, Thomas P. Simon Iv, Mitt Denney

Southeastern Fishes Council Proceedings

The diversity and distribution of fish species occurring in the Choctawhatchee River drainage in southeastern Alabama and northcentral Florida were surveyed to obtain historical baseline information. Three hundred seventy-four sites were evaluated for species diversity and distribution in the drainage, including compilation of unpublished records from southeastern natural history museums. The greatest diversity at any single site was 37 species. Sixty-eight sites were represented by 15 species or more, and 26 sites were represented by a single species. The most frequently encountered species includes Gambusia holbrooki, Percina nigrofasciata, Esox americanus, Notropis texanus, Lepomis macrochirus, Cyprinella n. sp. cf venusta, …

Quantitative Depth Profile Of Prochlorococcus In The Pacific Ocean, Benjamin Carter Calfee

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Prochlorococcus is a genus of extremely abundant marine cyanobacterium. This microbe is responsible for the majority of the primary production within marine environments and is thought to be the single most abundant photosynthetic organism. In addition to accounting for such a large portion of the world’s photosynthetic activity, Prochlorococcus functions with a genome much smaller than most other primary producers. Thus due to its general abundance and overall importance in oceanic ecosystems, an experiment was prompted to derive the spatial and numerical separation of the members of this genus across the Pacific Ocean through the use of quantitative polymerase chain …

Design Of An Underwater Video And Gps Mapping System For The Exploration Of Streambed Aquatic Populations, William Barbour

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

This design project was initiated due to the need for an underwater video mapping system optimized for exploration along the streambed or riverbed and under rocks. Such a system would be used for population mapping of fish and other aquatic species. The system also needed to incorporate global positioning system data with the visual data. The current prototype successfully fulfills the requirements for underwater practicality and visual and GPS data collection. It incorporates a compact waterproof camera with Sony lens, two Fenix high-intensity waterproof flashlights for illumination, a handheld DVR for recording video, and a Garmin 60CSx GPS unit. The …

Front Matter, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Post-Hurricane Katrina Survey For The Blackmouth Shiner (Notropis Melanostomus) At Historical Localities In Mississippi, Martin T. O'Connell, Anne M. Usee O'Connell

Southeastern Fishes Council Proceedings

No abstract provided.

Reproductive Biology Of The Frecklebelly Darter, Percina Stictogaster (Teleostei: Percidae), David J. Eisenhour, Audrey M. Richter, Lynn V. Eisenhour, Carol-Rose Gingras

Southeastern Fishes Council Proceedings

No abstract provided.

Minutes, Business Meeting, 37th Annual Meeting, Southeastern Fishes Council, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Rediscovery Of A Lost Paratype Of The Boulder Darter, Etheostoma Wapiti, Michael H. Doosey

Southeastern Fishes Council Proceedings

No abstract provided.

2011 Treasurer's Report For The Southeastern Fishes Council, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Diversity And Activity Of Roseobacters And Roseophage, Charles Ryan Budinoff

Doctoral Dissertations

Bacteria of the Roseobacter lineage are dominant bacterioplankton in coastal systems and contribute significantly to secondary production in oceanic environments. Generalities of Roseobacter ecology, diversity, and distributions are known, but the intraspecific differences between species and their dynamics over short temporal periods is not well understood. Bacteriophage that infect Roseobacters (‘roseophage’) have the potential to shunt secondary production into the dissolved carbon pool and through the process of infection alter Roseobacter physiology. Despite their significance, little effort was made prior to the onset of this study to characterize roseophage. Using culture dependent and independent approaches, I describe the diversity and …

The Effects Of Nutrient Limitation And Cyanophage On Heterotrophic Microbial Diversity, Claire Elyse Campbell

Masters Theses

Marine viruses are critically important in the regulation of biogeochemical cycles and host microbial communities. In this study, we tested whether the indirect effects of virus predation on a phototroph (i.e., Synechococcus) affected the composition of co-occurring heterotrophic bacteria under nitrogen and phosphorus limitation in long-term chemostat experiments. Using 454 Titanium barcoded pyrosequencing of the 16S rRNA gene, microbial diversity and technical (i.e., sequencing) reproducibility were assessed for nine individual chemostats across five different time points. A total of 325,142 reads were obtained; 194,778 high-quality, non-cyanobacterial sequences were assigned to 110 OTUs. Our results show high reproducibility …

Number 53 (December 2011), Southern Fishes Council

Southeastern Fishes Council Proceedings

(December 2011) - Population Status and Environmental Associations of the Rare Striated Darter, Etheostoma striatulum. By Andrew C. Abernathy and Hayden T. Mattingly

Conservation Status of the Longhead Darter, Percina macrocephala, in Kinniconick Creek, Kentucky. By David A. Eisenhour, Audrey M. Richter and Joshua M. Shiering

First Observation of a Natural Hybrid Between Endangered Roanoke Logperch (Percina rex) and Chainback Darter (Percina navisense). By James H. Roberts

Minutes, Business Meeting, 36th Annual Meeting, Southeastern Fishes Council

2010 Treasurer's Report for the Southeastern Fishes Council

Population Status And Environmental Associations Of The Rare Striated Darter, Etheostoma Striatum, Andrew C. Abernathy, Hayden T. Mattingly

Southeastern Fishes Council Proceedings

No abstract provided.

Front Matter, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

First Observation Of A Natural Hybrid Between Endangered Roanoke Logperch (Percina Rex) And Chainback Darter (Percina Navisense), James H. Roberts

Southeastern Fishes Council Proceedings

No abstract provided.

2010 Treasurer's Report For The Southeastern Fishes Council, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Conservation Status Of The Longhead Darter, Percina Macrocephala, In Kinnicock Creek, Kentucky, David A. Eisenhour, Audrey M. Richter, Joshua M. Shiering

Southeastern Fishes Council Proceedings

No abstract provided.

Minutes, Business Meeting, 36th Annual Meeting, Southeastern Fishes Council, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Invasion Of Gulf Menhaden In The Alabama River, T. Heath Hayley, R. Kyle Bolton, Carol E. Johnston

Southeastern Fishes Council Proceedings

No abstract provided.

Minutes, Business Meeting, 35th Annual Meeting, Southeastern Fishes Council, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Southeastern Fishes Council State Reports, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Front Matter, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Number 52 (December 2010), Southern Fishes Council

Southeastern Fishes Council Proceedings

(December 2010) - Comparative Conservation Genetics of Two Endangered Darters, Percina rex and Percina jenkinsi By Anna L. George, David Neely, and Richard Mayden

Invasion of Gulf Menhaden in the Alabama River By T. Heath Haley, R. Kyle Bolton, and Carol E. Johnston

Southeastern Fishes Council State Reports

Minutes, Business Meeting, 35th Annual Meeting, Southeastern Fishes Council

2009 Treasurer's Report for the Southeastern Fishes Council

Comparative Conservation Genetics Of Two Endangered Darters, Percina Rex And Percina Jenkinsi, Anna L. George, David A. Neely, Richard L. Mayden

Southeastern Fishes Council Proceedings

No abstract provided.

2009 Treasurer's Report For The Southeastern Fishes Council, Southeastern Fishes Council

Southeastern Fishes Council Proceedings

No abstract provided.

Number 51 (December 2009), Southern Fishes Council

Southeastern Fishes Council Proceedings

(December 2009) - Post-impoundment Changes in the Cyprinid Fauna of the Lower Sabine River, Louisiana and Texas By Royal D. Suttkus and Maurice F. Mettee

The Desperate Dozen: Southeastern Freshwater Fishes on the Brink. By Bernard R. Kuhajda, Anna L. George, and James D. Williams

Prioritizing Areas of the Conasauga River Sub-basin in Georgia and Tennessee for Preservation and Restoration. By Seth J. Wenger, Megan M. Hagler, and Byron J. Freeman.

State Southeastern Fishes Council State Reports

Minutes, Business Meeting, 34th Annual Meeting, Southeastern Fishes Council

2008 Treasurer's Report for the Southeastern Fishes Council