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Full-Text Articles in Life Sciences

Status Of The Major Oyster Diseases In Virginia 1996 A Summary Of The Annual Monitoring Program, Lisa M. Ragone Calvo, Eugene M. Burreson Mar 1997

Status Of The Major Oyster Diseases In Virginia 1996 A Summary Of The Annual Monitoring Program, Lisa M. Ragone Calvo, Eugene M. Burreson

Reports

No abstract provided.


Development Of A Protein-Free Chemically Defined Culture Medium For The Propagation Of The Oyster Pathogen Perkinsus Marinus, Jf Lapeyre, M Faisal Jan 1997

Development Of A Protein-Free Chemically Defined Culture Medium For The Propagation Of The Oyster Pathogen Perkinsus Marinus, Jf Lapeyre, M Faisal

VIMS Articles

In the present study we describe a protein-free, chemically defined culture medium, designated JL-ODRP-3, which supports the propagation of Perkinsus marinus, a parasite of the eastern oyster, Crassotrea virginica. P. marinus adapted rapidly to the defined medium and the growth rate of the protozoan increased significantly following a few subcultures. Two isolates of P. marinus, one from the Chesapeake Bay (Virginia) and the other from the Gulf of Mexico (Texas) were cultured for at least ten passes. The doubling times far the isolates from Virginia and Texas, in Jog phase, were 18 +/- 1.2 and 28.6 +/- 3.2 hours respectively, …


Parasitological Examination Of Wasting Disease In Black Abalone, Haliotis Cracherodii : Final Report, Jeffrey D. Shields, Frank O. Perkins Jan 1997

Parasitological Examination Of Wasting Disease In Black Abalone, Haliotis Cracherodii : Final Report, Jeffrey D. Shields, Frank O. Perkins

Reports

Black abalone in southern California are afflicted with chronic intestinal infections of a rickettsia-like organism that causes a debilitating and fatal withering syndrome. The hematology of withered animals indicated that cellular degradation and apoptosis occurred in tandem with the · decline and catabolism of abalone tissues. Two types of hemocytes were found in the hemolymph. Type I and Type II Hyalinocytes were distinguished by subtle differences in their cytoplasmic vesicles. Densities of both types of hemocytes declined in abundance, and small, presumptive stem cells increased in abundance with the progression of the disease. No circulating granulocytes were present in hemolymph; …