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Full-Text Articles in Life Sciences

Protocol For Enrichment Of The Membrane Proteome Of Mature Tomato Pollen, Puneet Paul, Palak Chaturvedi, Anida Mesihovic, Arindam Ghatak, Wolfram Weckwerth, Enrico Schleiff Jun 2017

Protocol For Enrichment Of The Membrane Proteome Of Mature Tomato Pollen, Puneet Paul, Palak Chaturvedi, Anida Mesihovic, Arindam Ghatak, Wolfram Weckwerth, Enrico Schleiff

Department of Agronomy and Horticulture: Faculty Publications

We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of Solanum lycopersicum (tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched fraction was then subjected to shotgun proteomics for identification of proteins, followed by in silico analysis to annotate and classify the detected proteins.


Potassium Applications And Yellow Shoulder Disorder Of Tomatoes In High Tunnels, Elizabeth Maynard, Israel S. Calsoya, Jill Malecki Jan 2017

Potassium Applications And Yellow Shoulder Disorder Of Tomatoes In High Tunnels, Elizabeth Maynard, Israel S. Calsoya, Jill Malecki

Purdue Fruit and Vegetable Research Reports

Tomatoes grown in unheated high tunnels can suffer significant loss in quality due to yellow shoulder disorder, particularly in susceptible varieties. This paper reports on a preliminary trial to determine whether incidence and severity of the disorder could be influenced by applying potassium fertilizers. Plots with higher leaf potassium content tended to have less fruit with severe yellow shoulder disorder, but increasing the amount of potassium fertilizer applied did not significantly reduce the disorder. From this study it isn’t possible to make specific recommendations for reducing yellow shoulder disorder with potassium applications.


Potassium Applications And Yellow Shoulder Disorder Of Tomatoes In High Tunnels, Elizabeth Maynard, Israel S. Calsoya, Jill Malecki Jan 2017

Potassium Applications And Yellow Shoulder Disorder Of Tomatoes In High Tunnels, Elizabeth Maynard, Israel S. Calsoya, Jill Malecki

Midwest Vegetable Trial Reports

Tomatoes grown in unheated high tunnels can suffer significant loss in quality due to yellow shoulder disorder, particularly in susceptible varieties. This paper reports on a preliminary trial to determine whether incidence and severity of the disorder could be influenced by applying potassium fertilizers. Plots with higher leaf potassium content tended to have less fruit with severe yellow shoulder disorder, but increasing the amount of potassium fertilizer applied did not significantly reduce the disorder. From this study it isn’t possible to make specific recommendations for reducing yellow shoulder disorder with potassium applications.