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Rapid In Vitro Multiplication Of Non-Runnering Fragaria Vesca Genotypes From Seedling Shoot Axillary Bud Explants, Babul C. Sarker, Douglas D. Archbold, Robert L. Geneve, Sharon T. Kester

Horticulture Faculty Publications

Fragaria vesca L. has become a model species for genomic studies relevant to important crop plant species in the Rosaceae family, but generating large numbers of plants from non-runner-producing genotypes is slow. To develop a protocol for the rapid generation of plants, leaf explants were compared to single axillary bud shoot explants, both from in vitro-grown Fragaria vesca seedlings, as sources of shoots for new plant production in response to benzyladenine (BA) or thidiazuron (TDZ) combined with indolebutyric acid (IBA) on Murashige and Skoog’s Basal Salt (MS) medium. BA at 2.0 and 4.0 mg L⁻¹ and TDZ at 1.5 …