Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 5 of 5

Full-Text Articles in Life Sciences

Substrates Of The Arabidopsis Thaliana Protein Isoaspartyl Methyltransferase 1 Identified Using Phage Display And Biopanning, Tingsu Chen, Nihar Nayak, Susmita Maitra Majee, Jonathan Lowenson, Kim R. Schäfermeyer, Alyssa C. Eliopoulos, Taylor D. Lloyd, Randy Dinkins, Sharyn E. Perry, Nancy R. Forsthoefel, Steven G. Clarke, Daniel M. Vernon, Zhaohui Sunny Zhou, Tomas Rejtar, A. Bruce Downie Nov 2010

Substrates Of The Arabidopsis Thaliana Protein Isoaspartyl Methyltransferase 1 Identified Using Phage Display And Biopanning, Tingsu Chen, Nihar Nayak, Susmita Maitra Majee, Jonathan Lowenson, Kim R. Schäfermeyer, Alyssa C. Eliopoulos, Taylor D. Lloyd, Randy Dinkins, Sharyn E. Perry, Nancy R. Forsthoefel, Steven G. Clarke, Daniel M. Vernon, Zhaohui Sunny Zhou, Tomas Rejtar, A. Bruce Downie

Horticulture Faculty Publications

The role of protein isoaspartyl methyltransferase (PIMT) in repairing a wide assortment of damaged proteins in a host of organisms has been inferred from the affinity of the enzyme for isoaspartyl residues in a plethora of amino acid contexts. The identification of PIMT target proteins in plant seeds, where the enzyme is highly active and proteome long-lived, has been hindered by large amounts of isoaspartate-containing storage proteins. Mature seed phage display libraries circumvented this problem. Inclusion of the PIMT co-substrate, S-adenosylmethionine (AdoMet), during panning permitted PIMT to retain aged phage in greater numbers than controls lacking co-substrate or when …


Horticulture, Timothy A. Woods Oct 2010

Horticulture, Timothy A. Woods

Agricultural Economics Presentations

No abstract provided.


Inhibitors Of Plant Peptide Deformylase For Use As Broad-Spectrum Herbicides And Methods For Identifying The Same, Robert L. Houtz, Lynnette M. A. Dirk, Mark Alan Wiliams Jun 2010

Inhibitors Of Plant Peptide Deformylase For Use As Broad-Spectrum Herbicides And Methods For Identifying The Same, Robert L. Houtz, Lynnette M. A. Dirk, Mark Alan Wiliams

Horticulture Faculty Patents

The invention relates to a method of identifying herbicides and to the use of inhibitors of plant peptide deformylase as broad spectrum herbicides.


Modeling Water Use In Nursery Crops, Amy Fulcher Jan 2010

Modeling Water Use In Nursery Crops, Amy Fulcher

University of Kentucky Doctoral Dissertations

Water use is an important topic in the global agriculture community and is a critical input in nursery crop production. Several plants in the genus Cornus are important nursery crops. Not only are they economically relevant, they are found in grafted and seedling forms and parents and their hybrid are readily available in the trade, facilitating an assessment of water requirements. Anecdotal information suggests that Cornus taxa have differing stress tolerance and water use requirements. Research was conducted to characterize and model water use among Cornus taxa. Scanning electron microscopy and anatomy‐based micromorphological studies as well as transpiration chamber‐based studies …


Copy Number Variation Shapes Genome Diversity In Arabidopsis Over Immediate Family Generational Scales, Seth Debolt Jan 2010

Copy Number Variation Shapes Genome Diversity In Arabidopsis Over Immediate Family Generational Scales, Seth Debolt

Horticulture Faculty Publications

Arabidopsis thaliana is the model plant and is grown worldwide by plant biologists seeking to dissect the molecular underpinning of plant growth and development. Gene copy number variation (CNV) is a common form of genome natural diversity that is currently poorly studied in plants and may have broad implications for model organism research, evolutionary biology, and crop science. Herein, comparative genomic hybridization (CGH) was used to identify and interrogate regions of gene CNV across the A. thaliana genome. A common temperature condition used for growth of A. thaliana in our laboratory and many around the globe is 22 degrees C. …