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Isolated Spinach Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit ΕN-Methyltransferase And Method Of Inactivating Ribulose-1,5-Bisphosphatase Carboxylase/Oxygenase Large Subunit ΕN-Methyltransferase Activity, Robert L. Houtz Jun 1999

Isolated Spinach Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit ΕN-Methyltransferase And Method Of Inactivating Ribulose-1,5-Bisphosphatase Carboxylase/Oxygenase Large Subunit ΕN-Methyltransferase Activity, Robert L. Houtz

Horticulture Faculty Patents

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup.ε N-methyltransferase (protein methylase III or Rubisco LSMT) from a plant which has a des(methyl) lysyl residue in the LS is disclosed. In addition, the full-length cDNA clones for Rubisco LSMT are disclosed. Transgenic plants and methods of producing same which have the Rubisco LSMT gene inserted into the DNA are also provided. Further, methods of inactivating the enzymatic activity of Rubisco LSMT are also disclosed.


Cloning And Developmental Expression Of Pea Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit Epsilon N-Methyltransferase, Robert L. Houtz Feb 1999

Cloning And Developmental Expression Of Pea Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit Epsilon N-Methyltransferase, Robert L. Houtz

Horticulture Faculty Patents

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup.ε N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the ε-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant …