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Horticulture

University of Kentucky

Horticulture Faculty Publications

DNA methylation

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Full-Text Articles in Life Sciences

Common Garden Experiment Reveals Altered Nutritional Values And Dna Methylation Profiles In Micropropagated Three Elite Ghanaian Sweet Potato Genotypes, Belinda Akomeah, Marian D. Quain, Sunita A. Ramesh, Lakshay Anand, Carlos M. Rodríguez López Apr 2019

Common Garden Experiment Reveals Altered Nutritional Values And Dna Methylation Profiles In Micropropagated Three Elite Ghanaian Sweet Potato Genotypes, Belinda Akomeah, Marian D. Quain, Sunita A. Ramesh, Lakshay Anand, Carlos M. Rodríguez López

Horticulture Faculty Publications

Micronutrient deficiency is the cause of multiple diseases in developing countries. Staple crop biofortification is an efficient means to combat such deficiencies in the diets of local consumers. Biofortified lines of sweet potato (Ipomoea batata L. Lam) with enhanced beta-carotene content have been developed in Ghana to alleviate Vitamin A Deficiency. These genotypes are propagated using meristem micropropagation to ensure the generation of virus-free propagules. In vitro culture exposes micropropagated plants to conditions that can lead to the accumulation of somaclonal variation with the potential to generate unwanted aberrant phenotypes. However, the effect of micropropagation induced somaclonal variation on …


Salt Stress Induces Non-Cg Methylation In Coding Regions Of Barley Seedlings (Hordeum Vulgare), Moumouni Konate, Michael J. Wilkinson, Benjamin T. Mayne, Stephen M. Pederson, Eileen S. Scott, Bettina Berger, Carlos M. Rodriguez Lopez Jun 2018

Salt Stress Induces Non-Cg Methylation In Coding Regions Of Barley Seedlings (Hordeum Vulgare), Moumouni Konate, Michael J. Wilkinson, Benjamin T. Mayne, Stephen M. Pederson, Eileen S. Scott, Bettina Berger, Carlos M. Rodriguez Lopez

Horticulture Faculty Publications

Salinity can negatively impact crop growth and yield. Changes in DNA methylation are known to occur when plants are challenged by stress and have been associated with the regulation of stress-response genes. However, the role of DNA-methylation in moderating gene expression in response to salt stress has been relatively poorly studied among crops such as barley. Here, we assessed the extent of salt-induced alterations of DNA methylation in barley and their putative role in perturbed gene expression. Using Next Generation Sequencing, we screened the leaf and root methylomes of five divergent barley varieties grown under control and three salt concentrations, …