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Full-Text Articles in Life Sciences
Defective Aba-Mediated Sugar Signalling Pathway In An Established Arabidopsis Thaliana Cell Suspension Culture Explains Its Stay-Green Phenotype At High Sugar Concentrations, Avery Mccarthy
Electronic Thesis and Dissertation Repository
An unusual sugar insensitive phenotype was identified in an established cell suspension culture of Arabidopsis thaliana. We characterized the physiology, biochemistry and genetics of the sugar insensitive cell culture, in order to identify factors contributing to the phenotype. Chlorophyll levels of the cell suspension culture were insensitive to high sucrose (6-15% w/v) and maintained a green phenotype. Immunoblotting indicated that levels of key photosynthetic proteins (PsaA, Lhcb2 and Rubisco) increased as a function of external sucrose concentration. The green cell culture was photosynthetically competent based on light-dependent, CO2-saturated rates of O2 evolution as well as Fv/Fm …
Glucan Synthase-Like 8: A Key Player In Early Seedling Development In Arabidopsis, Behnaz Saatian
Glucan Synthase-Like 8: A Key Player In Early Seedling Development In Arabidopsis, Behnaz Saatian
Electronic Thesis and Dissertation Repository
Plants’ cell walls have unique chemical composition and features which enable them to play essential roles during plant development as shaping the cells and providing intercellular communication between adjacent cells. Polysaccharides, including callose, and glycoproteins are known as the main constituents of the cell wall. Callose, a linear β-1,3-glucan polymer, is accumulated at the cell plate during cytokinesis, in plasmodesmata, where it regulates cell-to-cell communication, in dormant phloem, where it seals sieve plates after mechanical injury and pathogen attack, and in male and female gametophytes. GLUCAN SYNTHASE-LIKE (GSL) genes in Arabidopsis comprise a family of 12 members. A …
Arabidopsis Chromatin Remodeler Brahma: Its Functional Interplay With Polycomb Proteins And The Ref6 Histone Demethylase, Chenlong Li
Electronic Thesis and Dissertation Repository
BRAHMA (BRM) is a SWI/SNF-type chromatin remodeling ATPase that plays an important role in regulation of gene expression. Tri-methylation of lysine 27 on histone H3 (H3K27me3) is a histone modification that is associated with transcriptionally repressed genes and catalyzed by Polycomb Group (PcG) proteins. BRM has been proposed to antagonize the function of PcG proteins but the underlying molecular mechanism is unclear. To understand how BRM regulates the function of PcG proteins during plant development, a genome-wide analysis of H3K27me3 in brm mutant was performed using chromatin immunoprecipitation followed by next generation sequencing (ChIP-seq). Loss of BRM leads to increased …
Two Spotted Spider Mite (Tetranychus Urticae) Selection To Arabidopsis Thaliana, Huzefa Ratlamwala
Two Spotted Spider Mite (Tetranychus Urticae) Selection To Arabidopsis Thaliana, Huzefa Ratlamwala
Electronic Thesis and Dissertation Repository
Spider mite feeding on A. thaliana induces the production of indole glucosinolates (IGs), plant secondary metabolites that negatively affect mite performance. In this study I conducted selection experiments on A. thaliana with varying levels of IGs, to determine if mites could adapt to IGs and other defense compounds. After 12 months, mites reared on host with IGs performed significantly better on A. thaliana than mites maintained on beans. However, an adaptation cost was detected between selected mite lines and their ancestral host. The qRT-PCR data on different mite lines revealed that the detoxification genes previously identified may only be involved …
Arabidopsis Thaliana-Spider Mite Interaction: Plant Perception, Signalling, And Response, Kristie A. Bruinsma
Arabidopsis Thaliana-Spider Mite Interaction: Plant Perception, Signalling, And Response, Kristie A. Bruinsma
Electronic Thesis and Dissertation Repository
The two-spotted spider mite, Tetranychus urticae, is a cell-content feeding chelicerate herbivore, feeding on over 1000 plant species, one of which is Arabidopsis thaliana. This research uses microarray data from two A. thaliana accessions that differ in susceptibility to spider mite feeding to identify how the plant defends itself against this herbivore. Mutant analysis of induced plant defense pathways and physiological assays of mite performance indicate that A. thaliana utilizes: a) damage associated molecular pattern receptors, PEPR1 and PEPR2, to aid in perception of attack; b) jasmonic acid as the key phytohormone involved in resistance signalling; and c) …