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Genetics and Genomics

Electronic Theses and Dissertations

2013

Genomic evolution

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Isolation And Characterization Of A Full Length Retrotransposon: Cr1, Cassandra Michelle Weason Jan 2013

Isolation And Characterization Of A Full Length Retrotransposon: Cr1, Cassandra Michelle Weason

Electronic Theses and Dissertations

Transposable elements (TE) have been found in all genomes and have clearly had a major impact on genomic evolution. The described research takes advantage of an abundant transposable element present in the genomes of Anseriformes, called Chicken Repeat 1 (CR1). Previous studies in Anseriformes suggest that CR1 is presently active in recent evolutionary time (St. John, 2004). A fully functional CR1 element itself is approximately 4.5kb long (Kajikawa, 1997), where almost all inserts are truncated at the 5' end. Because of this, it has been a challenge to isolate a full length, active element. In this study, two CR1 sequences …