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Novel Protein Secretion And Chitin Utilization Machinery Of Flavobacterium Johnsoniae, Sampada Suresh Kharade
Novel Protein Secretion And Chitin Utilization Machinery Of Flavobacterium Johnsoniae, Sampada Suresh Kharade
Theses and Dissertations
Flavobacterium johnsoniae, a member of phylum Bacteroidetes, is a gliding bacterium that digests insoluble chitin. A novel protein secretion system, the Type IX secretion system (T9SS), secretes the motility adhesins SprB and RemA and is also required for chitin utilization. In order to understand F. johnsoniae chitin utilization and the role of the T9SS, Fjoh_4555 (chiA) was targeted for analysis. Disruption of chiA resulted in cells that failed to digest chitin and complementation restored this ability. Antisera raised against ChiA were used to characterize its secretion. ChiA was secreted in soluble form by wild-type cells but remained cell-associated in T9SS …
Evaluation Of Alternative Culling Strategies For Maintenance Of Genetic Variaton In Bison (Bison Bison), Rachael Marie Toldness
Evaluation Of Alternative Culling Strategies For Maintenance Of Genetic Variaton In Bison (Bison Bison), Rachael Marie Toldness
Theses and Dissertations
Bison (Bison bison) once numbered in the millions and roamed across much of the lower 48 states. By the late 1800s, overhunting had reduced the population to around 1,000 individuals. Strong efforts to establish managed herds have resulted in a steady bison population increase. Currently, six herds are maintained by the U.S. Fish and Wildlife Service (FWS) at National Wildlife Refuges (NWRs) and are intensively managed through annual culling to keep herd size at targeted levels. Although various criteria have historically been used to select individuals for culling, the FWS currently employs an allele frequency based strategy that we have …