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Full-Text Articles in Life Sciences
Risk Assessment And Regulation Of Bacteriophage As A New Food Antimicrobial Agent, Lin Wei, Juan-Juan Sun
Risk Assessment And Regulation Of Bacteriophage As A New Food Antimicrobial Agent, Lin Wei, Juan-Juan Sun
Food and Machinery
Based on the theory of modern risk regulation and the existing research results at home and abroad, this paper discusses the bacteriophage as a kind of food antimicrobial agent from the perspective of risk assessment and risk regulation. In previous studies on risk assessment, no obvious adverse effects and side effects of phages on the environment, animals and human body were found. As an antibacterial agent, bacteriophage has shown good antibacterial effects in fruits and vegetables, meat, dairy products and seafood, with a very broad application prospect. However, while complying with the application research trend of bacteriophage food antimicrobial agents, …
Application Of A Bacteriophage–Sanitizer Combination In Post-Harvest Control Of E. Coli O157:H7 Contamination On Spinach Leaves In The Presence Or Absence Of A High Organic Load Produce Wash, Badrinath Vengarai Jagannathan, Steven Kitchens, Stuart Price, Melissa Morgan, Paul Priyesh Vijayakumar
Application Of A Bacteriophage–Sanitizer Combination In Post-Harvest Control Of E. Coli O157:H7 Contamination On Spinach Leaves In The Presence Or Absence Of A High Organic Load Produce Wash, Badrinath Vengarai Jagannathan, Steven Kitchens, Stuart Price, Melissa Morgan, Paul Priyesh Vijayakumar
Animal and Food Sciences Faculty Publications
Foodborne illness due to the consumption of contaminated products continues to be a serious public health issue. Bacteriophages might provide a natural and effective way to control and reduce the pathogenic bacterial population on food products. Researchers have conducted various experiments to prove their effectiveness against different pathogens and their ability to act as a natural intervention to control pathogen populations, especially in the food industry. In this study, a cocktail of bacteriophages (phages) was added to wash water in the presence of a high organic load along with commercially used sanitizers (chlorine or Sanidate 5.0) to study the efficacy …
Efficacy Of Bacteriophage Cocktail To Control E. Coli O157:H7 Contamination On Baby Spinach Leaves In The Presence Or Absence Of Organic Load, Badrinath Vengarai Jagannathan, Steven Kitchens, Paul Priyesh Vijayakumar, Stuart Price, Melissa Morgan
Efficacy Of Bacteriophage Cocktail To Control E. Coli O157:H7 Contamination On Baby Spinach Leaves In The Presence Or Absence Of Organic Load, Badrinath Vengarai Jagannathan, Steven Kitchens, Paul Priyesh Vijayakumar, Stuart Price, Melissa Morgan
Animal and Food Sciences Faculty Publications
Fruits and vegetables are high in nutrients that are essential for a healthy lifestyle. However, they also harbor an extensive array of microorganisms such as bacteria, which can be beneficial, neutral, or pathogenic. Foodborne pathogens can contaminate produce at any stage from the farm to the consumer’s table. Appropriate washing techniques using sanitizers can reduce the risk of pathogen contamination. Issues related to maintaining concentration, efficacy, and other problems have been a challenge for the food industry and, when left unresolved, have led to different outbreaks of foodborne illnesses. In this study, the efficacy of a lytic bacteriophage cocktail was …
Potential For Bacteriophage Cocktail To Complement Commercial Sanitizer Use On Produce Against Escherichia Coli O157:H7, Badrinath Vengarai Jagannathan, Steven Kitchens, Paul Priyesh Vijayakumar, Stuart Price, Melissa Morgan
Potential For Bacteriophage Cocktail To Complement Commercial Sanitizer Use On Produce Against Escherichia Coli O157:H7, Badrinath Vengarai Jagannathan, Steven Kitchens, Paul Priyesh Vijayakumar, Stuart Price, Melissa Morgan
Animal and Food Sciences Faculty Publications
The increasing concern for food safety has created a need to evaluate novel techniques to eliminate or control pathogens, resulting in safe food. In this study, four bacteriophages of bovine origin, specific to E. coli O157:H7, were successfully isolated and characterized. A microplate reader assay demonstrated the efficacy of the bacteriophage (phage) cocktail against E. coli O157:H7 resulting in a significant reduction (p < 0.01) in the target pathogen population. The phage cocktail demonstrated significant efficacy (p < 0.05) against E. coli O157:H7 in the presence of the most utilized sanitizers in the United States, namely 100 parts per million (ppm) free chlorine and 100-ppm peroxyacetic acid. Survival in the sanitizer concentrations demonstrates the potential …
Uv-C Treatment On The Safety Of Skim Milk: Effect On Microbial Inactivation And Cytotoxicity Evaluation, Danielle M. Ward, Ankit Patras, Agnes Kilonzo-Nthenge, Sudheer K. Yannam, Che Pan, Hang Xiao, Michael Sasges
Uv-C Treatment On The Safety Of Skim Milk: Effect On Microbial Inactivation And Cytotoxicity Evaluation, Danielle M. Ward, Ankit Patras, Agnes Kilonzo-Nthenge, Sudheer K. Yannam, Che Pan, Hang Xiao, Michael Sasges
Agricultural and Environmental Sciences Faculty Research
The efficacy of UV-C irradiation as a nonthermal processing method for skim milk (SM) was investigated. SM inoculated with two surrogate viruses (MS2 and T1UV), and three bacteria (Escherichia coli ATCC 25922, Salmonella enterica serovar Typhimurium ATCC 13311, and Listeria monocytogenes ATCC 19115) was treated with Deanflow UV-C irradiation, a spiral reactor with the fluid pumped around a central low-pressure mercury UV lamp (40 W) emitting at 254 nm wave-length. A series of known UV doses (0–168.33 mJ·cm2) were delivered to the samples. The microbial loads of MS2, T1UV, E. coli, Salmonella, and Listeria were …
Microbial Inactivation And Cytotoxicity Evaluation Of Uv Irradiated Coconut Water In A Novel Continuous Flow Spiral Reactor, Manreet Singh Bhullar, Ankit Patras, Agnes Kilonzo-Nthenge, Bharat Pokharel, Sudheer K. Yannam, Kanyasiri Rakariyatham, Che Pan, Hang Xiao, Michael Sasges
Microbial Inactivation And Cytotoxicity Evaluation Of Uv Irradiated Coconut Water In A Novel Continuous Flow Spiral Reactor, Manreet Singh Bhullar, Ankit Patras, Agnes Kilonzo-Nthenge, Bharat Pokharel, Sudheer K. Yannam, Kanyasiri Rakariyatham, Che Pan, Hang Xiao, Michael Sasges
Agricultural and Environmental Sciences Faculty Research
A continuous-flow UV reactor operating at 254 nm wave-length was used to investigate inactivation of microorganisms including bacteriophage in coconut water, a highly opaque liquid food. UV-C inactivation kinetics of two surrogate viruses (MS2, T1UV) and three bacteria (E. coli ATCC 25922, Salmonella Typhimurium ATCC 13311, Listeria monocytogenes ATCC 19115) in buffer and coconut water were investigated (D10 values ranging from 2.82 to 4.54 mJ·cm− 2). A series of known UV-C doses were delivered to the samples. Inactivation levels of all organisms were linearly proportional to UV-C dose (r2 > 0.97). At the highest dose …
Efficacy Of Ultraviolet (Uv-C) Light In Reducing Foodborne Pathogens And Model Viruses In Skim Milk, Danielle M. Gunter-Ward, Ankit Patras, Manreet Singh Bhullar, Agnes Kilonzo-Nthenge, Bharat Pokharel, Michael Sasges
Efficacy Of Ultraviolet (Uv-C) Light In Reducing Foodborne Pathogens And Model Viruses In Skim Milk, Danielle M. Gunter-Ward, Ankit Patras, Manreet Singh Bhullar, Agnes Kilonzo-Nthenge, Bharat Pokharel, Michael Sasges
Agricultural and Environmental Sciences Faculty Research
The efficacy of low wavelength ultraviolet light (UV-C) as a disinfection process for a scattering fluid such as skim milk was investigated in this study. UV-C inactivation kinetics of two surrogate viruses (bacteriophages MS2 and T1UV) and three bacteria Escherichia coli ATCC 25922, S. Typhimurium ATCC 13311, Listeria monocytogenes ATCC 19115 in buffer and skim milk were investigated. UV-C irradiation was applied to stirred samples, using a collimated beam operating at 253.7 nm wavelength. A series of known UV-C doses (0–40 mJ·cm−2) were delivered to the samples except MS2 where higher doses (0–150 mJ·cm−2) were delivered. …
Acoustic Emission Of Lactococcus Lactis Ssp. Lactis C2 Infected With Three Bacteriophages C2, Sk1 And Ml3, Luxi Meng
Theses and Dissertations--Animal and Food Sciences
The objective of this research was to monitor the Acoustic Emission (AE) produced by Lactococcus lactis ssp. lactis C2 infected with three bacteriophages (c2, sk1 and ml3 at 90 min) using an acoustic emission-monitoring device which was designed at University of Kentucky in Lexington KY. The acoustic emission data was collected and then analyzed. These Acoustic Emission (AE) data suggested that bacteriophage ml3, sk1 and c2 can easily be distinguished by the differences in Absolute Energy (ABE), Centroid Frequency (CF) and Peak Frequency (PF) signals. The AE data suggested that bacteriophage sk1 and c2 caused greater stress on the lactis …
Antimicrobial Susceptibility Of Listeria Monocytogenes To Bacteriophage Listex™ P100 In Alfalfa Sprouts (Medicago Sativa), Tushar Sawant
Antimicrobial Susceptibility Of Listeria Monocytogenes To Bacteriophage Listex™ P100 In Alfalfa Sprouts (Medicago Sativa), Tushar Sawant
Food Science (MS) Theses
The seed germination process during sprout production provides suitable environmental conditions for the growth of pathogenic bacteria, such as Listeria monocytogenes. A potential way to control this bacterial growth is through the use of bacteriophages, which are naturally occurring viruses that specifically attack bacterial targets and have been shown to be effective antimicrobials in some foods. Therefore, the objective of this study was to evaluate the antimicrobial susceptibility of L. monocytogenes to bacteriophage on alfalfa sprouts during seed germination and subsequent refrigerated storage at 4 °C. Alfalfa sprout seeds were dip-inoculated with 5.5 x 105 CFU/ml L. monocytogenes serogroups 1 …
Isolation, Characterization And Application Of Bacteriophage To Treat Hydrogen Sulfide Producing Bacteria In Raw Animal Materials Destined For The Rendering Process, Chao Gong
All Theses
In the United States, billions of pounds of animal by-products are generated by the food processing industry every year. Hydrogen sulfide producing bacteria (SPB) can utilize the sulfur-containing proteins and amino acids in the raw animal materials destined for the rendering process to produce harmful hydrogen sulfide (H2S) gas rapidly under the ambient conditions, resulting in hazardous working environments and inferior quality of finished products. In this study, the application of bacteriophage was explored as an effective solution for the elimination of H2S production in the rendering industry. The objectives of this study were to: 1) isolate and characterize strains …
Development Of A Whey-Based Lactic Culture Medium Capable Of Bacteriophage Inhibition, Chao Tung Cheng
Development Of A Whey-Based Lactic Culture Medium Capable Of Bacteriophage Inhibition, Chao Tung Cheng
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
A whey product has been formulated for use as a lactic starter medium. Phosphate-treated whey medium (PWM) has been shown to support growth of lactic cultures and prevent phage proliferation. Comparisons were made of a commercial phage inhibitory medium (PIM), reconstituted non-fat dry milk (NDM) and PWM. PWM inhibited all phages tested and stimulated starter growth. PWM was not as stimulating as PIM but was better than NDM. Good Cheddar cheese has been made using PWM. PWM is more economical than PIM but NDM is the most economical one if cheese yield is considered.