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Full-Text Articles in Life Sciences
Analysis Of Protein Extraction And Antioxidant Activity Of Enzymatic Hydrolysates From Scallop Processing By-Products, Guo Yu, Wen Shi-Yu, Ni Ce, Ou Xiao-Hui, Qu Ting-Min, Wu Ying, He Shu-Wen, Li Hong-Hui, Cui Bo, Cheng Yun-Hui, Wen Li
Analysis Of Protein Extraction And Antioxidant Activity Of Enzymatic Hydrolysates From Scallop Processing By-Products, Guo Yu, Wen Shi-Yu, Ni Ce, Ou Xiao-Hui, Qu Ting-Min, Wu Ying, He Shu-Wen, Li Hong-Hui, Cui Bo, Cheng Yun-Hui, Wen Li
Food and Machinery
Objective: This study aimed to improve the utilization value of scallop processing by-products, by analyzing the antioxidant activity of its protein hydrolysates. Methods: The processing by-products of Argopecten irradians were used as raw materials, and the proteins were extracted by alkali extraction and acid precipitation, alkali extraction and ammonium sulfate precipitation and low temperature ethanol denaturation precipitation. Then the proteins were analyzed by SDS-PAGE. Pepsin, flavourzyme, dispase and alcalase were used to hydrolyze the protein, and the antioxidant activities of the hydrolysate products were analyzed. The molecular weight distribution, amino acid composition and hydroxyproline content of dispase hydrolysates …
Phenolic Compounds Profile And Antioxidant Activities Of Different Fractions Of Tartary Buckwheat, Chen Yue, Zhu Yong, Qin Li-Kang
Phenolic Compounds Profile And Antioxidant Activities Of Different Fractions Of Tartary Buckwheat, Chen Yue, Zhu Yong, Qin Li-Kang
Food and Machinery
Objective: This study aimed to investigate the phenolic compounds profile and antioxidant activities of different fractions of Tartary buckwheat. Methods: Tartary buckwheat was ground and sieved into shell, bran and powder. Phenolic compounds in tartary buckwheat were extracted with 70% ethanol, and phenolic compounds composition and antioxidant activity of the extract were analyzed. Results: In Tartary buckwheat bran, the total phenolics and flavonoids were (3 042±31) mg GAE/100 g·DW and (5 290±233) mg RE/100 g·DW, accounting for 60.8%, 67.7% of the total Tartary buckwheat, respectively. Rutin was the dominant phenolic compounds in bran extract, and the content …
Primary Structure Characterization And Antioxidant Activity Of Polysaccharide From Zizyphus Jujuba Cv. Banzao, Li Nan, Zhang Xiangfei, Yang Chunjie
Primary Structure Characterization And Antioxidant Activity Of Polysaccharide From Zizyphus Jujuba Cv. Banzao, Li Nan, Zhang Xiangfei, Yang Chunjie
Food and Machinery
Objective:This study aimed to analyze the physicochemical properties, primary structure and antioxidant activity of polysaccharide from Zizyphus jujuba cv. Banzao.Methods:The polysaccharides were prepared by water extraction and alcohol precipitation, deproteinization with trichloroacetic acid and decolorization with hydrogen peroxide. The physicochemical properties of polysaccharides were studied, and the possible structures were predicted by Ultraviolet-visible (UV-Vis) and infrared (IR) spectroscopy. Finally, the free radical scavenging ability was determined.Results:The polysaccharide from Z. jujuba cv. Banzao contained the content of total sugar and uronic acid with 52.30% and 46.22%, respectively, and showed 64.70% of esterification degree, which was an …
Effects Of Nacl Stress And Cacl2 Supplemental On Phenolic Accumulation And Antioxidant Activity In Common Vetch Sprouts, Zhao Qi-Yan, Tang Ning, Zhao De-Chen, Zhang Bo-Ya, Cheng Yong-Qiang
Effects Of Nacl Stress And Cacl2 Supplemental On Phenolic Accumulation And Antioxidant Activity In Common Vetch Sprouts, Zhao Qi-Yan, Tang Ning, Zhao De-Chen, Zhang Bo-Ya, Cheng Yong-Qiang
Food and Machinery
Objective: The study aimed to develop the vegetable value of common vetch (CV) and explore the effects of salt solution on phenolic accumulation and antioxidant activity in CV sprouts.Methods: The effects of NaCl stress and supplemental CaCl2 on free and combined phenolic accumulation in CV sprouts were analysed by UPLC in comparison with pea sprouts, soybean sprouts and mung bean sprouts. The changes of key enzyme activities and gene relative expression levels of PAL, C4H and 4CL, antioxidant enzyme activities and antioxidant activities were investigated under NaCl and CaCl2 treatments.Results: The total phenolics increased from (10.63±0.40) mg GAE/g …
Anti Oxidant Capacity And Immuno Enhancing Act Ivity Oflycium Bar Barum Protoplasm, Yang Lu, Zhao Ran, Wu Zhen-Huai, Yuji An-Qiang, Yaang Jia-Mei
Anti Oxidant Capacity And Immuno Enhancing Act Ivity Oflycium Bar Barum Protoplasm, Yang Lu, Zhao Ran, Wu Zhen-Huai, Yuji An-Qiang, Yaang Jia-Mei
Food and Machinery
Objective：This study focused on the antioxidant andimmune enhancing effects of L.bar barumpu ree.Methods：Theantioxidant capacity of L.bar barum puree was measured byes-tab lishing an acute liver injury model.The immune activity of L.bar barum protoplasmwas evaluated by measuring the index ofimmune organs， the content of serum hemolysin and the degreeof plantar thickening.Results：Both low and medium doses of L.bar barum puree could significantly increase the content of glut a-thione peroxidase(GSH PX) in serum(P＜0.05， P＜0.01) andglutathione(GSH) in liver tissue(P＜0.05) ， and significantlydecrease the content ofprotein carbonyl in liver tissue ofmicewith acute alcoholic liver injury(P＜0.05， P＜0.01)andthecontent of malondialdehyde(MDA) (P＜0.05， P＜0.01) …
Optimization Of Extraction Process And Antioxidant Activity Of Flavonoids From Artemisia Argyi, Wang Na, Zhang Hao-Yu, Ning Can-Can, Fan Yun-Tao, Wang Lin-Qing, Yu Qiu-Ying
Optimization Of Extraction Process And Antioxidant Activity Of Flavonoids From Artemisia Argyi, Wang Na, Zhang Hao-Yu, Ning Can-Can, Fan Yun-Tao, Wang Lin-Qing, Yu Qiu-Ying
Food and Machinery
Objective:To optimize the extraction process of flavonoids from Artemisia argyi and evaluate the antioxidant activity in vitro. Methods:The ultrasonic-microwave combined water extraction method of Artemisia flavonoids was determined, and the extraction process was studied with the yield of total flavonoid, DPPH radical scavenging rate and ·OH radical scavenging rate of Artemisia argyi. Based on the single factor experiment, the factors with greater influence were screened through Plackett-Burman design, then optimized extraction process by response surface test. Results:The best extraction process was ultrasonic power 340 W, ultrasonic time 27 min, microwave power 600 W, microwave time 120 s, …
Research On Fermentation Process Optimization, Antioxidant And Hypoglycemic Activity Of Pigmented Rice Lactic Acid Beverage, Liu Ting, Li Jia-Ni, Shen Jun-Li, Lu Jun, Yang Wen-Yi
Research On Fermentation Process Optimization, Antioxidant And Hypoglycemic Activity Of Pigmented Rice Lactic Acid Beverage, Liu Ting, Li Jia-Ni, Shen Jun-Li, Lu Jun, Yang Wen-Yi
Food and Machinery
Objective: To develop pigmented rice lactic acid beverage with good antioxidant and hypoglycemic activity. Methods: Taking black and purple rice as the main material, the effect of mass ratio of purple rice to black rice, the addition amount of Lactobacillus bacteria, fermentation time and solid-liquid ratio on the sensory evaluation value, total phenolics content, ferric ion reducing antioxidant power (FRAP), ABTS free radical scavenging ability, and the inhibition rate of α-glucosidase of pigmented rice lactic acid beverage were explored. Results: The optimal technology of pigmented rice lactic acid beverage was as follows: the mass ratio of purple …
Study On Antioxidant Activities Of Proteins And Enzymatic Hydrolysates Of Eight Edible Fungi, Wang Yao-Ran, Zhao Yan, Chen Ming-Jie, Wei Chen-Ying, Zha Lei, Li Zhi-Ping
Study On Antioxidant Activities Of Proteins And Enzymatic Hydrolysates Of Eight Edible Fungi, Wang Yao-Ran, Zhao Yan, Chen Ming-Jie, Wei Chen-Ying, Zha Lei, Li Zhi-Ping
Food and Machinery
Objective: This study focused on the antioxidant activities of eight edible fungiproteins and their hydrolysates. Methods: Eight edible fungi proteins were hydrolyzed using alkaline protease. DPPH free radical scavenging activity, ABTS free radical scavenging activity, Fe2+ chelation rate and reducing power were used to evaluate antioxidant activity. Results: SDS-PAGE electrophoretic profile results indicated that alkaline protease was efficient in hydrolyzing proteins into low molecular weight peptides and amino acid; the antioxidant activity of the alkaline proteolysis product of Volvariellavolvacea was the highest with DPPH free radical scavenging ability (5 140.45±5.35) μg Trolox/g, ABTS free radical scavenging …
Extraction Of Mulberry Polysaccharide By Ultrasonic Cell Crusher-Assisting And Its Antioxidant Activity Analysis, Zhang Qiu-Hong
Extraction Of Mulberry Polysaccharide By Ultrasonic Cell Crusher-Assisting And Its Antioxidant Activity Analysis, Zhang Qiu-Hong
Food and Machinery
Objective:The cell crusher-assisted hot water extraction and antioxidant activity of mulberry polysaccharide were investigated. Methods:Extraction time, solid-liquid ratio, ultrasonic time, ultrasonic power and extraction temperature were used as the test conditions, the optimum extraction conditions of mulberry polysaccharide were investigated by four factors and three levels of orthogonal test. The antioxidant capacity of mulberry polysaccharide was determined by comparing the scavenging rates of VC and mulberry polysaccharide to DPPH· and hydroxyl radical (·OH). Results: The results showed that the highest yield of mulberry polysaccharide was (6.63±0.07)%, when the extraction time was 30 min,the ultrasonic power was 160 W, …
Optimization Of Microwave-Assisted Extraction Of Total Flavonoids From Leaves Of Moringa Oleifera Lam. By Response Surface Methodology And Its Antibacterial And Antioxidant Activities, Yang Li-Hua, Dong Xiao-Qing, Dong Qi-Lu, Li Xiao-Jiao
Optimization Of Microwave-Assisted Extraction Of Total Flavonoids From Leaves Of Moringa Oleifera Lam. By Response Surface Methodology And Its Antibacterial And Antioxidant Activities, Yang Li-Hua, Dong Xiao-Qing, Dong Qi-Lu, Li Xiao-Jiao
Food and Machinery
Objective:The purpose of this study is to improve the extraction rate of total flavonoids from the leaves of Moringa oleifera Lam. and evaluate their in vitro antibacterial and antioxidant capacity. Methods:The extraction of total flavonoids from the leaves of M. oleifera Lam. from Baoshan city was optimized by Box-Behnken response surface methodology, and its antibacterial and antioxidant activities were evaluated. Results: The optimal conditions for microwave-assisted extraction of total flavonoids from leaves of M. oleifera Lam. were solid-liquid ratio1∶29 (g/mL), microwave power 567 W, microwave time3.6 min and ethanol fraction 50%, and the highest extraction rate of total …
Research Progress On The Effect Of Biological And Non-Thermal Processing On The Content Of Phenolic Compounds And Antioxidant Activity In Cereals And Miscellaneous Beans, Ma Zhan-Qian, Zhai Xiao-Tong, Tan Bin
Research Progress On The Effect Of Biological And Non-Thermal Processing On The Content Of Phenolic Compounds And Antioxidant Activity In Cereals And Miscellaneous Beans, Ma Zhan-Qian, Zhai Xiao-Tong, Tan Bin
Food and Machinery
Bioprocessing and non-thermal processing can improve the quality of cereals and miscellaneous beans, which significantly increase the content of phenols and antioxidant activities. This study systematically reviewed the effects of bioprocessing and non-thermal processing on phenolic compounds and antioxidant activities of cereal and soybean, and prospected the key research ideas for further development of cereal and soybean processing.
The Effect Of Drying Method On The Physical And Chemical Properties And Antioxidant Activity Of Extruded Wheat Bran, Wu Fan, Wang Zhan, Zhou Jian, Shen Wang-Yang, Jia Xi-Wu
The Effect Of Drying Method On The Physical And Chemical Properties And Antioxidant Activity Of Extruded Wheat Bran, Wu Fan, Wang Zhan, Zhou Jian, Shen Wang-Yang, Jia Xi-Wu
Food and Machinery
Objective:Using wheat bran as raw material, the extruded bran is obtained by extrusion stabilization treatment, and the best drying method for extruded bran is determined.Methods:The effects of three drying methods (fluidized bed drying, microwave drying, hot air drying) were studied on the basic components of extruded bran, water holding capacity, water solubility, swelling power, oil holding power, color value and other physical and chemical properties, oxidation resistance, total phenols and total flavonoids; Using antioxidant, total phenols and total flavonoids as indicators to determine the best drying method for extruded bran.Results:The three drying methods have no …
Study On Preparation Of Collagen Peptides From Micropterus Salmoides Skin And Its Antioxidant Activity, Han Meng-Yao, Li Xin-Yue, Wang Xiao-Mei, Wang Guo-Zhi, Ye Jin-Yun, Zhang Zhong-Shan
Study On Preparation Of Collagen Peptides From Micropterus Salmoides Skin And Its Antioxidant Activity, Han Meng-Yao, Li Xin-Yue, Wang Xiao-Mei, Wang Guo-Zhi, Ye Jin-Yun, Zhang Zhong-Shan
Food and Machinery
Objective:Collagen was extracted from the skin of Micropterus salmoides and used to explore the effect of enzyme types on the antioxidant activity of collagen peptides.Methods:Four kinds of enzymes were used, namely alkaline protease, neutral protease, papain and trypsin. The enzymatic hydrolysis of collagen Alkaline protease enzymatically hydrolyze collagen peptide (APP), Neutral protease enzymatically hydrolyze collagen peptide (NPP), Papain enzymatically hydrolyze collagen peptide(PP), Trypsin hydrolysis of collagen peptide(TP), were obtained and compared their total antioxidant activity, including reducing activity, DPPH, ·OH and O-2·free radical scavenging ability, ferrous ion chelating ability and lipid Oxidation inhibitory activity.Results:Four kinds …
Effect Of Sulfate Modificate On The Structure And Antioxidant Activity Of Jujube Polysaccharide, Gong Xiao-Pei, Zhang Jian, Guo Xiao-Bin, Mao Xiao-Ying, Zhang Lian-Fu
Effect Of Sulfate Modificate On The Structure And Antioxidant Activity Of Jujube Polysaccharide, Gong Xiao-Pei, Zhang Jian, Guo Xiao-Bin, Mao Xiao-Ying, Zhang Lian-Fu
Food and Machinery
Objective:This study aimed to explore the relationship between the structure and antioxidant activity of purified red date polysaccharide ZJP-1 and sulfated red date polysaccharide S-ZJP-1.Methods:Using remnant jujube as raw material, jujube polysaccharide (ZJP-1) was purified by hot water extraction, and then separated by DEAE-52 ion exchange chromatography and Sephadex-200 dextran gel column chromatography. Thereafter, red jujube polysaccharide sulfated derivative (S-ZJP-1) was obtain through chlorosulfonic acid-pyridine sulfated modification.Results:Both ZJP-1 and S-ZJP-1 are composed of three monosaccharides: glucose, arabinose, and galactose, but the ratios are different. The two polysaccharides had characteristic absorption peaks of polysaccharides, and S-ZJP-1 …
Study On Antioxidant Activity Of Extracts From Different Polar Solvents Of Extract Of Fermented Soya Beans, Liu Jun-Ze, Hu Li-Ming, Wang Ren-Guang, Wang Shu-Min, Wang Huan
Study On Antioxidant Activity Of Extracts From Different Polar Solvents Of Extract Of Fermented Soya Beans, Liu Jun-Ze, Hu Li-Ming, Wang Ren-Guang, Wang Shu-Min, Wang Huan
Food and Machinery
Objective:To explore the difference in composition and antioxidant activity of crude extracts of fermented soya beans with different solvent extracts.Methods:70% ethanol was used the extraction solvent, the fermented soya beans powder was extracted by ultrasonic method. After the solvent was evaporated, the crude extract was obtained. The crude extract was dispersed in water and extracted with petroleum ether, dichloromethane, ethyl acetate, and n-butanol in turn to obtain different solvent extracts. The colorimetry was used to determine the content of total flavonoids and total polysaccharides in crude extracts and different solvent extracts, HPLC was used to determine the …
Isolation And Identification Of Endophytic Bacteria From Fresh Gastrodia Elata And Preliminary Study On Its Functional Activity, Sun Hai-Yan, Liu Xiao, Pei Jin-Jin, Hao Dan-Qing, Wang Er-Nan
Isolation And Identification Of Endophytic Bacteria From Fresh Gastrodia Elata And Preliminary Study On Its Functional Activity, Sun Hai-Yan, Liu Xiao, Pei Jin-Jin, Hao Dan-Qing, Wang Er-Nan
Food and Machinery
Objective:This study aimed to elucidate the activities of endophytic bacteria and their metabolites of Gastrodia elata BI, and to provide theoretical reference for the development of new natural food antibacterial agents and antioxidants.Methods: The endophyte of fresh G. elata in Qinba area was isolated and purified by tissue homogenization and streaking method. The antibacterial, antioxidant and anti-tumor activities of the extract were determined. Furthermore, molecular biology identification was applied for the highly active strains.Results: In this study, four strains were isolated from the inside of fresh G. elata, among which, endophytic bacteria B1 and B2 have good …
Comparison Of Active Components And Antioxidant Activity Of Non-Medicinal Parts Of Polygonatum Cyrtonema, Wang Tian-Mei, Wang Hua-Lei, Li Dan-Dan, Chen Song-Shu, Pan Ke-Qin
Comparison Of Active Components And Antioxidant Activity Of Non-Medicinal Parts Of Polygonatum Cyrtonema, Wang Tian-Mei, Wang Hua-Lei, Li Dan-Dan, Chen Song-Shu, Pan Ke-Qin
Food and Machinery
Objective:This study focuses on improving the comprehensive utilization rate of Polygonatum cyrtonema.Methods:The contents of total flavonoids, total saponins and total phenols from roots, stems, leaves, flowers and buds, of P. cyrtonema were determined. The scavenging rates of DPPH·, ·OH and nitrites in extracts of above tissue were measured and compared as well. Results:The contents of total flavonoids and total phenols five different kinds of non-medicinal tissue were in the same order, in which from highest to lowest was, leaves, buds, flowers, stems and roots, and the content of total saponins in flower was the highest In terms of …
Research On Extraction Of Total Flavonoids From Green Tea With Deep Eutectic, Ni Xue-Hua, Wang Heng-Peng
Research On Extraction Of Total Flavonoids From Green Tea With Deep Eutectic, Ni Xue-Hua, Wang Heng-Peng
Food and Machinery
Objective:This study aimed to optimize the extraction technology of total flavonoids from green tea with Deep Eutectic Solvents (DESs) and evaluate the antioxidant activity of total flavonoids extracted from green tea under optimal conditions.Methods:The extraction rate of total flavonoids was taken as the index, with the ratio of liquid to solid, extraction temperature and extraction time taken as the influencing factors, and the extraction process parameters were optimized by orthogonal test, followed by the antioxidant activity evaluating with DPPH free radical inhibition.Results:The optimal extraction conditions for extraction of total flavonoids from green tea with DESs were 80% …
Optimizations Of Extraction And Separation Process Of Saponins And In Vitro Antioxidant Activity From The Female Gonad Of Apostichopus Japonicus, Zhong Jing-Shi, Zhang Jian, Liu Fang, Wang Gong-Ming, Wang Yi-Xin
Optimizations Of Extraction And Separation Process Of Saponins And In Vitro Antioxidant Activity From The Female Gonad Of Apostichopus Japonicus, Zhong Jing-Shi, Zhang Jian, Liu Fang, Wang Gong-Ming, Wang Yi-Xin
Food and Machinery
Objective: This study aimed to optimize the ultrasonic-assisted extraction technology and HSCCC separation process of saponins from the female gonad of Apostichopus japonicus and to explore their antioxidant activities. Methods: Extraction time, ethanol concentration, ultrasonic time, and solid-to-solvent ratio as the influencing factors were considered in this study, and the extraction process of saponin was optimized using the response surface methodology. The ratio of solvent systems, the volume flow of mobile phases, and the rotation speeds of HSCCC were optimized. The DPPH radical and ABTS radical scavenging experiments were conducted to evaluate the antioxidant activities of the crude extracts and …