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Full-Text Articles in Life Sciences

Patterns Of Population Structure And Introgression Among Recently Differentiated Drosophila Melanogaster Populations, Jenn M. Coughlan, Andrius J. Dagilis, Antonio Serrato-Capuchina, Hope Elias, David Peede, Kristin Isbell, Dean M. Castillo, Brandon S. Cooper, Daniel R. Matute Oct 2022

Patterns Of Population Structure And Introgression Among Recently Differentiated Drosophila Melanogaster Populations, Jenn M. Coughlan, Andrius J. Dagilis, Antonio Serrato-Capuchina, Hope Elias, David Peede, Kristin Isbell, Dean M. Castillo, Brandon S. Cooper, Daniel R. Matute

Nebraska Extension: Faculty and Staff Publications

Despite a century of genetic analysis, the evolutionary processes that have generated the patterns of exceptional genetic and phenotypic variation in the model organism Drosophila melanogaster remains poorly understood. In particular, how genetic variation is partitioned within its putative ancestral range in Southern Africa remains unresolved. Here, we study patterns of population genetic structure, admixture, and the spatial structuring of candidate incompatibility alleles across a global sample, including 223 new accessions, predominantly from remote regions in Southern Africa. We identify nine major ancestries, six that primarily occur in Africa and one that has not been previously described. We find evidence …


Lessons Learned From The 2019 Nebraska Floods: Implications For Emergency Management, Mass Care, And Food Security, Eric E. Calloway, Nadine B. Nugent, Katie L. Stern, Ashley Mueller, Amy L. Yaroch Sep 2022

Lessons Learned From The 2019 Nebraska Floods: Implications For Emergency Management, Mass Care, And Food Security, Eric E. Calloway, Nadine B. Nugent, Katie L. Stern, Ashley Mueller, Amy L. Yaroch

Nebraska Extension: Faculty and Staff Publications

This qualitative study aimed to understand the actions, challenges, and lessons learned for addressing the food and water needs of flood survivors, with a special focus on vulnerable populations and the implications for food security, to inform future disaster response efforts in the U.S. Semi-structured in-depth interviews were conducted from January to August 2020 with the local, state, and national stakeholders (n = 27) involved in the disaster response to the 2019 Nebraska floods, particularly those involved in providing mass care, such as food, water, and shelter, for the flood survivors. The challenge themes were related to limited risk …


Hybrid Incompatibility Between Ldrosophila Virilis And D. Lummei Is Stronger In The Presence Of Transposable Elements, Dean M. Castillo, Leonie C. Moyle Aug 2022

Hybrid Incompatibility Between Ldrosophila Virilis And D. Lummei Is Stronger In The Presence Of Transposable Elements, Dean M. Castillo, Leonie C. Moyle

Nebraska Extension: Faculty and Staff Publications

Mismatches between parental genomes in selfish elements are frequently hypothesized to underlie hybrid dysfunction and drive speciation. However, because the genetic basis of most hybrid incompatibilities is unknown, testing the contribution of selfish elements to reproductive isolation is difficult. Here, we evaluated the role of transposable elements (TEs) in hybrid incompatibilities between Drosophila virilis and D. lummei by experimentally comparing hybrid incompatibility in a cross where active TEs are present in D. virilis (TE+) and absent in D. lummei, to a cross where these TEs are absent from both D. virilis (TE−) and D. lummei genotypes. Using genomic data, …


Incorporating Chokeberry (Aronia) Into A Home Landscape, Claude J. Jean, Paul E. Read, Ellen T. Paparozzi Jan 2022

Incorporating Chokeberry (Aronia) Into A Home Landscape, Claude J. Jean, Paul E. Read, Ellen T. Paparozzi

Nebraska Extension: Faculty and Staff Publications

Chokeberry (Aronia) is an ornamental plant that has found use in the home landscape, providing colorful displays and annually producing berries for the enjoyment of the homeowner and wildlife alike. With careful placement, this durable plant needs minimal care and has few pest problems. It is gaining attention for its timeless beauty.


Testing The Drosophila Maternal Haploid Gene For Functional Divergence And A Role In Hybrid Incompatibility, Dean M. Castillo, Benjamin Mccormick, Connor M. Kean, Sahana Natesan, Daniel A. Barbash Jan 2022

Testing The Drosophila Maternal Haploid Gene For Functional Divergence And A Role In Hybrid Incompatibility, Dean M. Castillo, Benjamin Mccormick, Connor M. Kean, Sahana Natesan, Daniel A. Barbash

Nebraska Extension: Faculty and Staff Publications

Crosses between Drosophila simulans females and Drosophila melanogaster males produce viable F1 sons and poorly viable F1 daughters. Unlike most hybrid incompatibilities, this hybrid incompatibility violates Haldane’s rule, the observation that incompatibilities preferentially affect the heterogametic sex. Furthermore, it has a different genetic basis than hybrid lethality in the reciprocal cross, with the causal allele in Drosophila melanogaster being a large species-specific block of complex satellite DNA on its X chromosome known as the 359-bp satellite, rather than a protein-coding locus. The causal allele(s) in Drosophila simulans are unknown but likely involve maternally expressed genes or factors since the F1 …