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Isolation, Purification And Identification Of Ace Inhibitory Peptide From Paramisgurnus Dabryanus Protein, Dong Jing-Qi, He Ze-He, Tian Gui-Fang, Shao Juan-Juan, Wu Xiao-He
Isolation, Purification And Identification Of Ace Inhibitory Peptide From Paramisgurnus Dabryanus Protein, Dong Jing-Qi, He Ze-He, Tian Gui-Fang, Shao Juan-Juan, Wu Xiao-He
Food and Machinery
Objective: This study aimed to develop ACE inhibitory peptide of Paramisgurnus dabryanus.Methods: Ultrafiltration, Sephadex G-15 and RP-HPLC were used to isolate and purify ACE inhibitory peptides from the proteolysis products of P.dabryanus,and their structures were analyzed by HPLC-MS/MS. The inhibitory mode of purified ACE inhibitory peptide was analyzed by inhibition kinetics. Results: The amino acid sequence of ACE inhibitory peptide was Glu-Gly-HIS, the relative molecular weight was 341.2 Da, the IC 50 value was (17.89±3.28)μg/mL, and the purification ratio was 27.47. Conclusion: The inhibitory peptide of P. dabryanus is a competitive ACE inhibitory peptide.
Protective Effects Of The Pea Ace Inhibitory Peptide On Induced Damage Of Ea.Hy.926 Cells, Zhang Shuo, Zheng Xiqun, Liu Xiaolan, Wang Juntong
Protective Effects Of The Pea Ace Inhibitory Peptide On Induced Damage Of Ea.Hy.926 Cells, Zhang Shuo, Zheng Xiqun, Liu Xiaolan, Wang Juntong
Food and Machinery
Objective:This study aimed to realize the high-value utilization of pea protein.Methods:Using pea protein as raw material, ACE inhibitory peptide derived from pea protein was prepared by double-enzyme coordinated enzymatic hydrolysis method, and the ACE inhibition rate, hydrolysis degree and soluble protein content of the hydrolysate were used as indicators to determine the best enzymatic process for the pea ACE inhibitory peptide. Then the ACE inhibitory peptides produced under the optimal enzymatic digestion process were graded by ultrafiltration to explore the effect of molecular weight on the ACE inhibitory activity. Finally, the protective effect of the pea peptide with high ACE …
Study On The Preparation Of Ace Inhibitory Peptides By Probiotic Fermentation Of Quinoa, Li Shuai, Yuan Ya-Hong, Yue Tian-Li
Study On The Preparation Of Ace Inhibitory Peptides By Probiotic Fermentation Of Quinoa, Li Shuai, Yuan Ya-Hong, Yue Tian-Li
Food and Machinery
Objective: The potential ability of probiotics to prepare angiotensin-converting enzyme (ACE) inhibitory fermentation broth from quinoa and the effects of fermentation process on it were investigated. Methods: In this study, 18 strains of lactic acid bacteria and 12 strains of yeast were screened for their ability to prepare ACE inhibitory fermentation broth using quinoa as the raw material and the ACE inhibition rate as an indicator. The inoculum amount, fermentation temperature and fermentation time were optimized by orthogonal tests. Finally, the peptides in the fermentation broth were identified, and the selected peptides were tested in vivo to evaluate …
Enzymatic Preparation And Activity Study Of Rice Bran Protein-Derived Ace Inhibitory Peptides, Zhang Ling-Yu, Miao Jian-Yin, Cao Yu, Dai Wei-Jie, Liu Guo, Li Zhen-Wei, Cao Yong
Enzymatic Preparation And Activity Study Of Rice Bran Protein-Derived Ace Inhibitory Peptides, Zhang Ling-Yu, Miao Jian-Yin, Cao Yu, Dai Wei-Jie, Liu Guo, Li Zhen-Wei, Cao Yong
Food and Machinery
Objective:This study aimed to develop new rice bran products.Methods:The ACE inhibition rate was used as an indicator, and the rice bran protein was optimized by single factor and response surface experiments. The optimal enzyme hydrolysate peptides were separated by ultrafiltration, activity evaluation and amino acid composition analysis were performed.Results:The optimal process conditions were as follows: pH 7.2, mass substrate concentration 8.2 g/100 mL, enzymolysis at 46 ℃ for 3 h, and enzyme addition 0.3 g/100 g rice bran protein. Under the control of these conditions, the ACE inhibition rate of the enzymatic hydrolysate was (73.15±0.64)%, …