Life Sciences Commons^™

Liquid Holding Capacity And Structural Changes During Heating Of Fish Muscle: Cod (Gadus Morhua L.) And Salmon (Salmo Salar), Ragni Ofstad, Siw Kidman, Reidar Myklebust, Anne-Marie Hermansson

Food Structure

The loss of water and fat in cod and salmon muscle was studied as a function of heating temperature from 5-70 C. The liquid-holding capacity was measured by a low speed centrifugation net test leading to the separation of released liquid. To obtain a better understanding of the liquid-holding properties, the microscopic changes of the samples were evaluated by light microscopy. Two different preparation techniques were used . Cod lost twice as much water as salmon upon beating. After an initial delay , the water loss increased at 20-35°C, attained a maximum at 45-50°C, and thereafter decreased in both fish …

Microstructure Of Whey Protein/ Anhydrous Milkfat Emulsions, M. Rosenberg, S. L. Lee

Food Structure

Research has been undertaken in order to determine the microstructure of emulsions consisting of whey proteins and anhydrous milk fat. The results revealed that whey protein films were formed at oil/water interfaces. Scanning electron microscopy (SEM) analysis of chemically fixed specimens1 and cryo-SEM analysis of partially etched, fro zen specimens revealed that the ad sorbed protein layer (coating the fat droplets) had a granular, aggregated appearance. Emulsions with a high fat load prepared under high homogenization pressure exhibited a bimodal particle-size distribution. Clu!;tering of the protein-coated droplets, and sharing phenomena at the oil / protein interfaces were evident in these …

Whey Proteins As Microencapsulating Agents. Microencapsulation Of Anhydrous Milkfat - Structure Evaluation, M. Rosenberg, S. L. Young

Food Structure

Microencapsulation of milkfat may open new fields of application for this milk constituent by transforming it into dry and stable powder. Research has been undertaken to study the microstructure of whey protein based, anhydrous milkfat-containing, spray-dried microcapsules. Whey protein concentrates of 50% and 75% protein and whey protein isolate were evaluated as microencapsulating agents (wall materials). The effects of wall composition, fat load , and drying conditions on the capsule's structure were studied by scanning electron microscopy (SEM). Spherical capsules with smooth , wrinkle-free surfaces were observed in all cases. Whey protein isolate-based microcapsules dried at a temperature higher than …

Microstructural Studies In Fat Research, I. Heertje

Food Structure

Microstructural studies play an important role in establishing the relation between composition, processing and final properties of many food products. In order to arrive at a full description of microstructure many visualization- and preparation techniques are needed. A number of fatty products such as shortenings, margarine , butter, and low fat spreads are discussed from a microstructural point of view. Examples of the influence of process parameters on microstructure and rheological properties are given. In particular, attention is paid to the fat crystalline matrix and the emulsion structure.

Further, a new methodology is described making it possible to study interactions …

Practical Aspects Of Electron Microscopy In Dairy Research, Miloslav Kalab

Food Structure

Milk products are based mostly on casein micelles, fat globules, and whey proteins. The former two constituents are corpuscular and whey proteins become corpuscular when coagulated. Structural changes in these basic constituents during processing have been studied by electron microscopy. This review discusses the structures of yoghurt, curd, cheeses (hard cheeses, mould-ripened cheeses, cream cheeses, and process cheese), cream, milk powders, and nontraditional dairy products. Defects and deviations from traditional structures of these products are explained where the causes are known. Examples of such causes are foaming of milk, presence of unusual ingredients (bacterial polysaccharides, whey protein concentrates), and alterations …

Microstructure Of Mozzarella Cheese During Manufacture, Craig J. Oberg, William R. Mcmanus, Donald J. Mcmahon

Food Structure

Scanning electron microscopy was used to examine structural developments during the manufacture of low-moisture, part-skim Mozzarella cheese made by a stirred curd procedure. The micrographs showed changes in the protein matrix , dispersion of fat globules, and bacteria during processing. Most curd knitting occurred during the curd stirring step, particularly dry stirring. A thin (5 ,urn) curd skin was observed on curd particles at the end of dry stirring. Dry salting prior to stretching resulted in the rapid loss of whey from the curd particle. Protein fibers were aligned and longitudinal columns of whey and fat were formed when the …

Composition And Microstructure Of Commercial Full-Fat And Low-Fat Cheeses, V. V. Mistry, D. L. Anderson

Food Structure

The objective of this study was to analyze the composition of commercial full-fat and low-fat cheeses and to evaluate their microstructure. Commercial cheeses evaluated included full-fat and low-fat Cheddar, Mozzarella , processed, and Swiss cheeses. Cheddar cheeses ranged from 8.2% fat and 5 1.1% moisture in the 75% low-fat product to 33.2% fat and 35.9% moisture in the full-fat cheese . Mozzarella cheeses ranged in fat from a low of 2. I% to a high of 24% with corresponding moisture content of 56.6 to 45.5% respectively. Fatfree processed cheese had 0. 9% fat and 58.7% moisture , while the full …

Microstructure And Fat Extractability In Microcapsules Based On Whey Proteins Or Mixtures Of Whey Proteins And Lactose, D. L. Moreau, M. Rosenberg

Food Structure

Research was undertaken to determine the microstructure of whey protein/lactose-based , anhydrous milk fat- containing , spray -dried microcapsules and to study the relationship between core extractability and the state of lactose in these microcapsules. Spherical microcapsules nearly free of surface dents were obtained in a ll cases The milkfat was found to be organized in the form of small droplets (0.2-0.8 um) embedded in the capsule wall. The surface topography of the capsul es ex hibited features that may be li nked to removal of mil kfat drop lets from th e outer surfaces at early stages of the …

Relationship Between The Starch Granule Structure And The Textural Properties Of Heat-Induced Surimi Gels, V. Verrez-Bagnis, B. Bouchet, D. J. Gallant

Food Structure

Starch, used as a textural additive in heat-induced surimi gel, influences the rigidity of the protein gel matrix and hence the gel strength according to its botanical characteristics. The present study focuses on the correlations existing between the textural properties of heatind uced surimi gels obtained by physical measurements and the characteristics of different commercial starches. The gelatinization temperature of starch was closely related to the expressible moisture, work to fracture, and elongation. Behaviour of starchy components during thermal processing and its relationship to fish protein gel matrix were studied by light and electron microscopy. These studies showed differences in …

Changes In The Microstructure Of A Comminuted Meat System During Heating, Peter A. Koolmees, Gerrit Wijngaards, Monique H.G. Tersteeg, Jan G. Van Logtestijn

Food Structure

As part of a series of experiments on heating of a comminuted meat system , image analyses were conducted to quantify microstructural changes that could be visualized by light microscopy. Three different batches of comminuted meat were investigated. Two batches (A and B) were prepared without polyphosphate; one of these (B) was deliberately overchopped. One batch containing 0. I% polyphosphate was prepared (C). All batches were heated at I I different temperatures ranging from 40 to 115 °C. The microstructure of the three types of meat batte rs at the end of the com minu tion process differed. These types …

High-Resolution Scanning Electron Microscopy Of Milk Products: A New Sample Preparation Procedure, William R. Mcmanus, Donald J. Mcmahon, Craig J. Oberg

Food Structure

A metal-impregnation technique (tannin-ferrocyanide- osmium tetroxide) and thin metal coating imparted excellent preservation , and electric and thermal properties to milk products for scanning electron microscopy, resulting in high- resolution images. Three nanometer resolution was obtained, with minimal edge effects, charging , thermal drift, and decoration. Samples were fixed in 2% gluta raldehyde, cryoprotected in 70% ethanol, and cryofractured, exposing internal structure. Fat was extracted with Freon 113 and the sample were metal -impregnated with 1.0% osmium tetroxide and 1.5% potassium ferrocyanide, reduced with 1% hydroquinone, dried using !he critical-point drying melhod , and ion beam sputter-coated with 2-5 nm …