Life Sciences Commons^™

Microemulsions Of Cinnamon And Orange Oils As An Antimicrobial Delivery System For Salmonella Biofilms On Stainless Steel, Jennifer Vuia-Riser

Doctoral Dissertations

Essential oils (EOs) are natural antimicrobials that can be used to develop intervention strategies to inhibit pathogens, but EOs are lipophilic. Colloidal systems, such as microemulsions, are needed for food industry applications. This dissertation focused on the development and characterization of a microemulsion composed of cinnamon oil (CO, Cinnamomum zeylanicum) and orange oil (OO, Citrus sinensis) to be used against Salmonella Enteritidis H4267 biofilms formed on stainless steel disc surfaces.

First, the antimicrobial activity of CO and OO against S. Enteritidis H4267, either used in combination or in a microemulsion system, was determined. CO had a greater antimicrobial …

A Transcriptomic Analysis Of Salmonella Enterica Newport In Planta And After Postharvest Sanitization, Laurel Lynn Dunn

Doctoral Dissertations

The consumption of fresh produce is increasingly linked to incidence of foodborne illness. Pathogens including Salmonella enterica, Shiga toxigenic Escherichia coli, and Listeria monocytogenes are exposed to produce crops through direct human or animal contact, contaminated agricultural water, bioaerosols, run-off, and improperly treated compost. S. enterica has demonstrated exceptional tolerance to the stresses encountered in the environment, on plant tissue, and from postharvest antimicrobial mitigation strategies. Understanding the transcriptomic mechanisms S. enterica employs to survive these hazards is integral for the development of more effective preventive controls and post-harvest steps to prevent the pathogen from infecting consumers.

Salmonella In Low Water Activity Foods: Physiological, Genetic Modification And Control Methods, Wei Chen

Doctoral Dissertations

The objective of this study was to evaluate the expression of fatty acid associated genes (fabA, fabD and cfa) of five serovars of Salmonella exposed to sugar over a 14-day period. Changes in the fatty acid composition of Salmonella Tennessee in glycerol solutions of different water activity (a_w) (1.0-0.6) and the relationship between survival and fatty acid modification (as altered by exogenously supplied fatty acids) at a_w 1.0-0.6 were also determined. Furthermore, the antimicrobial activities of carvacrol, cinnamaldehyde, and lauric arginate (LAE) alone or in combination against Salmonella Tennessee in a laboratory model of …

Novel Rapid Molecular Detection And Processing Approaches For The Control Of Salmonella Enterica Serovars In The Food Environment, Chayapa Techathuvanan

Doctoral Dissertations

The increase in Salmonella enterica outbreaks calls for an urgent need to rapidly detect and control Salmonella-associated contamination. Loop-mediated isothermal amplification (LAMP) assay is a novel method that can be completed within 90 min in a simple waterbath. Detection is by simple turbidity, fluorescence, or gel electrophoresis and is more specific than PCR. Reverse-transcriptase LAMP (RT-LAMP) targeting mRNA for the potential detection of live infectious Salmonella or recent contamination was used in this study and detection sensitivity to culture-based detection and RT-PCR assays was compared in pure culture, food products, and food processing environments. Our results showed detection limits …