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Full-Text Articles in Life Sciences

Application Of A Bacteriophage–Sanitizer Combination In Post-Harvest Control Of E. Coli O157:H7 Contamination On Spinach Leaves In The Presence Or Absence Of A High Organic Load Produce Wash, Badrinath Vengarai Jagannathan, Steven Kitchens, Stuart Price, Melissa Morgan, Paul Priyesh Vijayakumar Dec 2021

Application Of A Bacteriophage–Sanitizer Combination In Post-Harvest Control Of E. Coli O157:H7 Contamination On Spinach Leaves In The Presence Or Absence Of A High Organic Load Produce Wash, Badrinath Vengarai Jagannathan, Steven Kitchens, Stuart Price, Melissa Morgan, Paul Priyesh Vijayakumar

Animal and Food Sciences Faculty Publications

Foodborne illness due to the consumption of contaminated products continues to be a serious public health issue. Bacteriophages might provide a natural and effective way to control and reduce the pathogenic bacterial population on food products. Researchers have conducted various experiments to prove their effectiveness against different pathogens and their ability to act as a natural intervention to control pathogen populations, especially in the food industry. In this study, a cocktail of bacteriophages (phages) was added to wash water in the presence of a high organic load along with commercially used sanitizers (chlorine or Sanidate 5.0) to study the efficacy …


Protein Oxidation In Foods: Mechanisms, Consequences, And Antioxidant Solutions, Mario Estévez, Youling L. Xiong Oct 2021

Protein Oxidation In Foods: Mechanisms, Consequences, And Antioxidant Solutions, Mario Estévez, Youling L. Xiong

Animal and Food Sciences Faculty Publications

No abstract provided.


Physicochemical And Microstructural Characterization Of Whey Protein Films Formed With Oxidized Ferulic/Tannic Acids, Yaosong Wang, Youling L. Xiong Jul 2021

Physicochemical And Microstructural Characterization Of Whey Protein Films Formed With Oxidized Ferulic/Tannic Acids, Yaosong Wang, Youling L. Xiong

Animal and Food Sciences Faculty Publications

Protein-based biodegradable packaging films are of environmental significance. The effect of oxidized ferulic acid (OFA)/tannic acid (OTA) on the crosslinking and film-forming properties of whey protein isolate (WPI) was investigated. Both of the oxidized acids induced protein oxidation and promoted WPI crosslinking through the actions of quinone carbonyl and protein sulfhydryl, and amino groups. OTA enhanced the tensile strength (from 4.5 MPa to max 6.7 MPa) and stiffness (from 215 MPa to max 376 MPa) of the WPI film, whereas OFA significantly increased the elongation at break. The water absorption capability and heat resistance of the films were greatly improved …


Partial Removal Of Phenolics Coupled With Alkaline Ph Shift Improves Canola Protein Interfacial Properties And Emulsion In In Vitro Digestibility, Jiang Jiang, Yunqing Nie, Xuemei Sun, Youling L. Xiong Jun 2021

Partial Removal Of Phenolics Coupled With Alkaline Ph Shift Improves Canola Protein Interfacial Properties And Emulsion In In Vitro Digestibility, Jiang Jiang, Yunqing Nie, Xuemei Sun, Youling L. Xiong

Animal and Food Sciences Faculty Publications

The effect of polyphenol removal (“dephenol”) combined with an alkaline pH shift treatment on the O/W interfacial and emulsifying properties of canola seed protein isolate (CPI) was investigated. Canola seed flour was subjected to solvent extraction to remove phenolic compounds, from which prepared CPI was exposed to a pH12 shift to modify the protein structure. Dephenoled CPI had a light color when compared with an intense dark color for the control CPI. Up to 53% of phenolics were removed from the CPI after the extraction with 70% ethanol. Dephenoled CPI showed a partially unfolded structure and increased surface hydrophobicity …


Nutraceutical Intervention With Colostrum Replacer: Can We Reduce Disease Hazard, Ameliorate Disease Severity, And Improve Performance In Preweaned Dairy Calves?, Melissa C. Cantor, D. L. Renaud, Joao H. C. Costa Mar 2021

Nutraceutical Intervention With Colostrum Replacer: Can We Reduce Disease Hazard, Ameliorate Disease Severity, And Improve Performance In Preweaned Dairy Calves?, Melissa C. Cantor, D. L. Renaud, Joao H. C. Costa

Animal and Food Sciences Faculty Publications

The objective of this randomized clinical trial was to determine if an intervention with colostrum replacer (CR; Premolac Plus, Zinpro; 125 g/d fed for 3 d) or a placebo milk replacer (Cows Match, Land O'Lakes Inc.; 125 g/d for 3 d) following a triggered alarm could ameliorate disease bouts in dairy calves. The alarm was set to detect negative deviations of milk intake (20% reduction) or drinking speed (30% reduction) in relation to a calf's 12-d rolling average feeding behavior. Calves were enrolled on this study (n = 42 CR, n = 42 placebo) when they triggered an alarm from …


Efficacy Of Bacteriophage Cocktail To Control E. Coli O157:H7 Contamination On Baby Spinach Leaves In The Presence Or Absence Of Organic Load, Badrinath Vengarai Jagannathan, Steven Kitchens, Paul Priyesh Vijayakumar, Stuart Price, Melissa Morgan Mar 2021

Efficacy Of Bacteriophage Cocktail To Control E. Coli O157:H7 Contamination On Baby Spinach Leaves In The Presence Or Absence Of Organic Load, Badrinath Vengarai Jagannathan, Steven Kitchens, Paul Priyesh Vijayakumar, Stuart Price, Melissa Morgan

Animal and Food Sciences Faculty Publications

Fruits and vegetables are high in nutrients that are essential for a healthy lifestyle. However, they also harbor an extensive array of microorganisms such as bacteria, which can be beneficial, neutral, or pathogenic. Foodborne pathogens can contaminate produce at any stage from the farm to the consumer’s table. Appropriate washing techniques using sanitizers can reduce the risk of pathogen contamination. Issues related to maintaining concentration, efficacy, and other problems have been a challenge for the food industry and, when left unresolved, have led to different outbreaks of foodborne illnesses. In this study, the efficacy of a lytic bacteriophage cocktail was …


Coccidia Vaccine Challenge And Exogenous Enzyme Supplementation In Broiler Chicken 1. Effect On Digesta Viscosity, Diet Energy Utilization, And Apparent Metabolizable Energy Value Of Wheat, Andrew E. Dunaway, Sunday A. Adedokun Feb 2021

Coccidia Vaccine Challenge And Exogenous Enzyme Supplementation In Broiler Chicken 1. Effect On Digesta Viscosity, Diet Energy Utilization, And Apparent Metabolizable Energy Value Of Wheat, Andrew E. Dunaway, Sunday A. Adedokun

Animal and Food Sciences Faculty Publications

This study examined the effect of exogenous mixed-enzyme supplementation (xylanase, β-glucanase, and pectinase) to a corn-SBM (CS) and a wheat-CS-based (WCS) diet in birds challenged with coccidia vaccine (Coccivac B-52™). The WCS-based diet was produced by replacing 30% of the energy-yielding portions of the CS-based diet with wheat. On day 14, 448 (n = 7) Cobb by-product breeder male broilers were assigned to a 2 (diet types) × 2 (with or without enzyme supplementation) × 2 (0 or 20 × coccidia vaccine challenge; CVC) factorial arrangement of treatments in a completely randomized design for the determination of the apparent …


Broiler Genetics Influences Proteome Profiles Of Normal And Woody Breast Muscle, Xue Zhang, K. Virellia To, Tessa R. Jarvis, Yan L. Campbell, Jasmine D. Hendrix, Surendranath Suman, Shuting Li, Daniel S. Antonelo, Wei Zhai, Jing Chen, Haining Zhu, M. Wes Schilling Jan 2021

Broiler Genetics Influences Proteome Profiles Of Normal And Woody Breast Muscle, Xue Zhang, K. Virellia To, Tessa R. Jarvis, Yan L. Campbell, Jasmine D. Hendrix, Surendranath Suman, Shuting Li, Daniel S. Antonelo, Wei Zhai, Jing Chen, Haining Zhu, M. Wes Schilling

Animal and Food Sciences Faculty Publications

Wooden or woody breast (WB) is a myopathy of the pectoralis major in fast-growing broilers that influences the quality of breast meat and causes an economic loss in the poultry industry. The objective of this study was to evaluate growth and proteome differences between 5 genetic strains of broilers that yield WB and normal breast (NB) meat. Eight-week-old broilers were evaluated for the WB myopathy and divided into NB and WB groups. Differential expression of proteins was analyzed using 2-dimensional gel electrophoresis and LC-MS/MS to elucidate the mechanism behind the breast myopathy because of the genetic …


Animal And Plant Protein Oxidation: Chemical And Functional Property Significance, Youling L. Xiong, Anqi Guo Jan 2021

Animal And Plant Protein Oxidation: Chemical And Functional Property Significance, Youling L. Xiong, Anqi Guo

Animal and Food Sciences Faculty Publications

Protein oxidation, a phenomenon that was not well recognized previously but now better understood, is a complex chemical process occurring ubiquitously in food systems and can be induced by processing treatments as well. While early research concentrated on muscle protein oxidation, later investigations included plant, milk, and egg proteins. The process of protein oxidation involves both radicals and nonradicals, and amino acid side chain groups are usually the site of initial oxidant attack which generates protein carbonyls, disulfide, dityrosine, and protein radicals. The ensuing alteration of protein conformational structures and formation of protein polymers and aggregates can result in significant …