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Proteolytic Activity Of Some Milk-Clotting Enzymes On K-Casein And K-Casein Macropeptide, Khalid M. Shammet
Proteolytic Activity Of Some Milk-Clotting Enzymes On K-Casein And K-Casein Macropeptide, Khalid M. Shammet
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
This work reviews studies of bovine K-casein and specifically K-casein macropeptide. Properties of K-casein, its structure and heterogeneity, proteolytic activity of some milk clotting enzymes on K-casein, and K-casein sensitive bonds are discussed. Macropeptides of other species are also presented. The carbohydrate moieties of bovine macropeptide together with their biological and physiological functions are reviewed.
Macropeptides were produced by enzymic hydrolysis from whole casein solution using crystalline chymosin (EC 3.4.23.4). Trichloroacetic acid (final concentrations 2, 8 and 12%) was added after 5, 30 and 60 min of incubation to precipitate protein and inactivate the enzyme. The filtrate was then exhaustively …