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Full-Text Articles in Life Sciences

Identification And Characterization Of Edible Cricket Peptides On Hypertensive And Glycemic In Vitro Inhibition And Their Anti-Inflammatory Activity On Raw 264.7 Macrophage Cells, Felicia Hall, Lavanya Reddivari, Andrea M. Liceaga Nov 2020

Identification And Characterization Of Edible Cricket Peptides On Hypertensive And Glycemic In Vitro Inhibition And Their Anti-Inflammatory Activity On Raw 264.7 Macrophage Cells, Felicia Hall, Lavanya Reddivari, Andrea M. Liceaga

Department of Food Science Faculty Publications

Recent studies continue to demonstrate the potential of edible insects as a protein base to obtain bioactive peptides applicable for functional food development. This study aimed at identifying antihypertensive, anti-glycemic, and anti-inflammatory peptides derived from the in vitro gastrointestinal digests of cricket protein hydrolysates. After sequential fractionation, the protein digest subfraction containing the lowest molecular weight (<0.5 kDa), hydrophobic (C18) and cationic peptides (IEX) was found responsible for the most bioactivity. The cationic peptide fraction significantly reduced (p < 0.05) α-amylase, α-glucosidase, and angiotensin converting enzyme (ACE) activity in vitro, and also inhibited the expression of NF-κB in RAW 264.7 macrophage cells. A total of 28 peptides were identified with mass spectrometry (LC–MS/MS) and de novo sequencing from the potent fraction. Three novel peptides YKPRP, PHGAP, and VGPPQ were chosen for the molecular docking studies. PHGAP and VGPPQ exhibited a higher degree of non-covalent interactions with the enzyme active site residues and binding energies comparable to captopril. Results from this study demonstrate the bioactive potential of edible cricket peptides, especially as ACE inhibitors.


Role Of Gut Microbiota In Anti-Colitic Effects Of Color-Fleshed Potatoes, Lavanya Reddivari, Shiyu Li, Tianmin Wang, Mary Kennett, Jairam Vanamala Jun 2020

Role Of Gut Microbiota In Anti-Colitic Effects Of Color-Fleshed Potatoes, Lavanya Reddivari, Shiyu Li, Tianmin Wang, Mary Kennett, Jairam Vanamala

Department of Food Science Faculty Publications

The prevalence of ulcerative colitis (UC), a chronic inflammatory bowel disease, is on the rise with ∼700,000 patients in the US alone in 2018. Gut bacterial dysbiosis plays an important role in ulcerative colitis. We have recently shown that anthocyanin-containing potatoes exert anti-inflammatory activity in colitic mice. However, no information is available on whether gut bacteria play a role in the anti-colitic activity of color-fleshed potatoes. This study examined the anti-colitic activity of red/purple-fleshed potatoes in mice with intact and antibiotic-ablated microbiome.


Maize Flavan-4-Ols And Anthocyanins Alleviated Dextran Sulfate Sodium-Induced Colitis In Mice Via Intestinal Barrier Function Restoration, Binning Wu, Shiyu Li, Haotian Chang, Ruth Anderson, Surinder Chopra, Lavanya Reddivari May 2020

Maize Flavan-4-Ols And Anthocyanins Alleviated Dextran Sulfate Sodium-Induced Colitis In Mice Via Intestinal Barrier Function Restoration, Binning Wu, Shiyu Li, Haotian Chang, Ruth Anderson, Surinder Chopra, Lavanya Reddivari

Department of Food Science Faculty Publications

This study investigated the anti-inflammatory effects of anthocyanins and flavan-4-ols (precursor of phlobaphenes) within a whole-food matrix against dextran sulfate sodium (DSS)-induced colitis using four maize near-isogenic lines (NILs) that differ only in a single class of flavonoids. The four NILs are A (lacks anthocyanins and phlobaphenes), B (phlobaphenes +), C (anthocyanins +) and D (anthocyanins + and phlobaphenes +).


Producer Willingness To Pay For Enhanced Packaging To Prevent Postharvest Decay Of Strawberries, Brian Coffey, Valentina Trinetta, Londa Nwadike, Umut Yucel Apr 2020

Producer Willingness To Pay For Enhanced Packaging To Prevent Postharvest Decay Of Strawberries, Brian Coffey, Valentina Trinetta, Londa Nwadike, Umut Yucel

Journal of Applied Farm Economics

We surveyed specialty crop producers in Kansas and Missouri to determine producer willingness to pay for new active packaging technology that prevents postharvest loss and increases shelf life. The survey also asked demographic questions to determine the producer and operation traits for this growing segment of production agriculture. More than half of those surveyed were female, and 60% were under 50 years of age. Smaller operations tend to utilize direct marketing and social media activity more than larger operations. Parametric willingness to pay estimates are approximately $0.39 per cardboard flat to purchase the antifungal film that increases shelf life of …