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Fructose 1,6-Bisphosphate Aldolase, A Novel Immunogenic Surface Protein On Listeria Species, Mercelo L. Mendonça, Gustavo Marçal Schmidt Garcia Moreira, Fabrício Fabrício Conceição, Michael Hust, Karla Sequeira Mendonça, Ângela Nunes Moreira, Rodrigo Correa Orrea França, Wladimir Padilha Da Silva, Arun K. Bhunia, José Antônio Guimarães Uimarães Aleixo
Fructose 1,6-Bisphosphate Aldolase, A Novel Immunogenic Surface Protein On Listeria Species, Mercelo L. Mendonça, Gustavo Marçal Schmidt Garcia Moreira, Fabrício Fabrício Conceição, Michael Hust, Karla Sequeira Mendonça, Ângela Nunes Moreira, Rodrigo Correa Orrea França, Wladimir Padilha Da Silva, Arun K. Bhunia, José Antônio Guimarães Uimarães Aleixo
Department of Food Science Faculty Publications
Listeria monocytogenes is a ubiquitous food-borne pathogen, and its presence in food or production facilities highlights the importance of surveillance. Increased understanding of the surface exposed antigens on Listeria would provide potential diagnostic and therapeutic targets. In the present work, using mass spectrometry and genetic cloning, we show that fructose-1,6-bisphosphate aldolase (FBA) class II in Listeria species is the antigen target of the previously described mAb-3F8. Western and dot blot assays confirmed that the mAb-3F8 could distinguish all tested Listeria species from close-related bacteria. Localization studies indicated that FBA is present in every fraction of Listeria cells, including supernatant and …