Life Sciences Commons^™

Dietary Carbohydrates Influence The Structure And Function Of The Intestinal Alpha-Glucosidases, Mohammad Chegeni

Open Access Dissertations

As the primary products of starch digestion by pancreatic α-amylase, maltooligosaccharides (including maltose) are the main substrates for the α-glucosidases at the intestinal brush border. Here, maltose was shown to induce the formation of a higher molecular weight (HMW) sucrase-isomaltase (SI) species in Caco-2 cells that sorts more quickly to the enterocyte surface to act as a digestive enzyme. As this finding suggested a maltose sensing ability of small intestinal enterocytes, molecular mechanisms associated with the maturation and trafficking of HMW SI were further investigated. A pulse-chase experiment using [ ³⁵S]-methionine revealed a higher rate of early trafficking and …

Quality And Safety Attributes Of Sun-Dried Raisins From Afghanistan, Stacy A. Mccoy

Open Access Theses

Raisins are an important export commodity for Afghanistan, however, Afghan packers are challenged to export to markets seeking high quality products due to limited knowledge regarding their quality and safety. To evaluate this, Afghan raisin samples from pre-, semi-, and post-processed raisins were obtained from a raisin packer in Kabul, Afghanistan. The raisins were analyzed and compared to U.S. standards for processed raisins. The samples tested did not meet U.S. import standards for embedded sand, but the process used to clean the raisins is suitable to maintain food safety standards.

Previous Afghan raisin shipments have been rejected from the U.S. …

Invasive Silver Carp (Hypophthalmichthys Moltrix) Protein Hydrolysates And Their Efficacy In Increasing Seedling Vigor And Reducing Seed Borne Pathogen Growth, Shanleigh Pauline Thomson

Open Access Theses

Silver carp are an invasive fish species introduced into the United States within the last forty years. Unable to be effectively eradicated or commercialized as a source of food, other methods must be developed to control and/or use these fish. Fish protein hydrolysates (FPH) are products derived from the proteins inherent within fish. Development of FPH from Silver carp for a variety of uses could aid in controlling their expanding population and provide organic produces with a seed treatment option which is not available to them currently.

In the first study, FPH were used as a seed vigor treatment for …

African Starchy Foods, Gastric Emptying, And Starch Digestion In Malian Stunted Children, Fatimata Cisse

Open Access Dissertations

Starch serves as the main energy source in cereal and tuber-rich diets, and its glycemic response profile has been associated with health-related conditions. Sorghum and millet are known to have relatively low starch digestibility, a potentially desirable property for controlling blood glucose response and providing sustained energy. Gastric emptying rates of traditional sorghum and millet-based African foods of the Sahelian region (couscous, thick and thin porridges made from millet and/or sorghum) were compared to those of non-traditional "modern" foods that are mostly consumed in urban areas using a non-invasive 13C-labelled octanoic acid breath test in healthy volunteers. The obtained results …

Formation Of Lactalbumin Nanoparticles By Desolvation Method, Menglu Gao, Jozef Kokini, Luis Fernando Maldonado-Mejia

The Summer Undergraduate Research Fellowship (SURF) Symposium

Protein nanoparticles are ideal carriers for bioactive compounds such as nutraceuticals and drugs because they are biodegradable, less immunogenic and non-toxic and can be nanoparticulated. This study focuses on the desolvation method to form lactalbumin protein nanoparticles. Lactalbumin is soluble in water and insoluble in many organic solvents. Different solvent/non-solvent ratios are evaluated in this research project for the effect they have on the size, PDI and stability of protein nanoparticles. Different methods including sonication and centrifugation were used and compared in terms of their effectiveness to produce small nanoparticles during fabrication of the nanoparticles. Data collected including protein nanoparticles …

Differences In Carbon Dioxide Evolution From Samples Of Shelled Corn Subjected To Various Storage Treatments, Cininta Alp Pertiwi

Open Access Theses

Shelled corn was collected from two sources - commercial grain elevators and corn harvested from Purdue University farms and then frozen until tested. Using a carbon dioxide (CO₂) test kit manufactured by Woods End Laboratories, CO₂ evolution measurements were conducted on shelled corn from both sources, after the samples were re-wetted to approximately 21% moisture content, placed in a 473 ml glass jar, and incubated at room temperature for 72 hours. The CO₂ test kit uses a gel that changes color in response to a change in the level of CO₂ in the surrounding air. …

Role Of Resveratrol Metabolites In Adipose Function, Mikaela Lynn Allan

Open Access Theses

The rise in obesity rate has drastically increased over the last few decades and has quickly grown into a worldwide epidemic. Large increases in visceral adipose tissue accumulation increase risk for metabolic disorders including insulin resistance, type 2 diabetes, and cardiovascular diseases. Currently, increased levels of circulating non-esterified fatty acids (NEFA) have been suggested to be the mediator linking obesity and type 2 diabetes. Studies identifying ways to alleviate mobilization of NEFA in adipose tissue via dietary phytochemicals may useful as a therapeutic approach. Despite the established benefits of resveratrol in health and in the development of obesity, the role …

Characterization Of Water-Solid Interactions In Crystalline Ingredients And Development Of Deliquescence Measurement Recommendations, Matthew C. Allan

Open Access Theses

There are five major mechanisms of water-solid interactions. The primary focus of this thesis was on two of these: deliquescence and hydrate formation. Many crystalline food ingredients are deliquescent compounds (e.g., NaCl, sucrose, and ascorbic acid) and some are both deliquescent and hydrate formers (e.g., glucose, thiamine HCl, citric acid). Deliquescence is the first order phase transformation of a crystalline solid to a solution above a critical relative humidity (RH) known as the deliquescence point (RH0). A crystalline hydrate is a pseudo-polymorph in which water is incorporated into the crystal structure, altering the molecular formula and the physical properties.^ To …

Evolution Of Food Quality Demand In The Food Service Industry In China: The Case Of Duck, Rachel Alison Carnegie

Open Access Theses

Booming economic growth and rising consumer incomes have impacted food preferences and purchasing behavior in China. At the same time, several internationally publicized food safety incidents, particularly in the animal husbandry sector, have heightened awareness of and concern for food safety and quality in meat and dairy. Rising quality demand and safety concerns have been studied at length in the food retail sector, but also appear to be important in the food service industry. This research uses data from a survey of duck restaurant managers and consumers in Beijing, Shanghai, Chengdu, and Guangzhou to determine preferences and willingness to pay …

High Voltage Atmospheric Cold Plasma Treatment Of Refrigerated Chicken Eggs For Control Of Salmonella Enteritidis On External Surfaces, Yi Chen

Open Access Theses

The global market for eggs is projected to reach 1.154 trillion by the year 2015. The number one risk to consumers from chicken eggs is contamination with Salmonella Enteritidis (SE). FDA estimates each year 142,000 illnesses in the U.S. are caused by SE contamination in chicken eggs. The first objective of this research study was to evaluate a novel High Voltage Atmospheric Cold Plasma (HVACP) technology in reducing externally inoculated SE on refrigerated chicken eggs. Raw, chicken eggs attached with SE population (10 7 CFU/ml), were placed in plastic containers filled with either dry Air (22%O2 , 78%N2 ) or …

Physical And Chemical Attributes Of A Genetically Modified Fruit Pectin, Carl Patrick Littrell

Open Access Theses

Pectin is an important polymer used in the food industry as a thickening and gelling agent. Though pectin is ubiquitous in plants, chemical and structural differences among pectin molecules prevent most from being viable for industrial use. Enzymes found naturally in fruit cell walls during the ripening process impair many desirable attributes of fruit pectins, rendering them unsuitable for industrial applications. Pectin methylesterase (PME) is one such enzyme whose expression can be altered during ripening through the use of recombinant genetic engineering. Reduction in levels of PME results in increased degree of methylation and molecular size of pectin, greatly increasing …

Fact Or Fiction: Random Mating In Field Populations Of Western Corn Rootworm (Diabrotica Virgifera Virgifera Leconte) Emerging On Bt And Refuge Corn Plants, Steven Joel Smith

Open Access Theses

The western corn rootworm, or WCR, (Diabrotica virgifera virgifera LeConte) is the most significant pest of field corn (Zea mays) in the United States, and has recently expanded its range into Europe. Since 2004, hybrid corn containing Bt toxins targeting the corn rootworm complex have been heavily adopted and are now the primary control measure for this pest in North American corn production. ^ The evolution of resistance is an ongoing concern, and to ensure Bt products will retain their usefulness, insect resistance management (IRM) tactics using various refuge structures have been adopted. One of the key …

Crystallization Inhibitor Properties Of Polymers And Effects On The Chemical And Physical Stability Of L-Ascorbic Acid During Preparation And Storage, Belinda Christina

Open Access Theses

Crystalline L-ascorbic acid (VitC) is an essential nutrient solid that is widely used in food applications for fortification, antioxidant, and label claim purposes. VitC is known as one of the most unstable vitamins, and its content must be declared on food labels. Interest has been increasing in creating amorphous solid structures for improving the solubility and/or dissolution rates of crystalline compounds. One of the most promising methods studied to prevent the crystallization of an amorphous solid is the use of polymers in dispersions, although the specific polymer properties that lead to the best physical stability for a particular type of …

Quality Changes In Hermetically Stored Corn Caused By Fungi And Sitophilus Zeamais, Nda-Agyima Addae-Mensah K

Open Access Theses

Hermetic storage has been shown to be effective in controlling insect pests and maintaining grain quality of dry grains at 13% moisture and below. However, the feasibility and use of hermetic storage for grains at intermediate mid-moisture levels under the influence of the sub-Saharan African weather conditions is relatively unknown. Hermetic storage experiments were conducted on grade 1 "6297 and 6333 VT RIB" hybrid corn under controlled temperature conditions at 10 degrees centigrade and 25 degrees centigrade at target approximate moisture content levels of 11, 15, 18 and 21% wet basis for a total storage period of 6 months. Corn …

Shrinkage Prediction During Pasta Dehydration, Gina Michelle Bressani

Open Access Theses

Shrinkage models from different fields of study were reviewed. Pasta was used as a model to further develop the hypothesis of predicting shrinkage in terms of the rate of relaxation and the rate of moisture loss. An extended literature review of pasta properties, such as isotherms, diffusivity, glass transition, rheological properties, and shrinkage was conducted. Isotherm and glass transition models were developed from the literature data acquired. Creep was tested with a 25 mm-diameter flat plate at 20 to 40°C at varying moisture content. Viscoelastic properties for the Burgers model for creep were determined, where higher moisture content samples resulted …

Fatty Acids And Mercury In Seventy Seven Species Of Commercially Available Finfish In The United States, Dennis P. Cladis

Open Access Theses

Finfish are consumed across the United States and constitute an important part of the American diet. However, seafood consumption can be a tenuous topic, with supporters highlighting the health benefits of eicosapentaenoic acid (EPA, C20:5n3) and docosahexaenoic acid (DHA, C22:6n3) and opponents emphasizing the neurotoxicity of methylmercury. Because all fish contain varying amounts of EPA, DHA, and methylmercury, the need for clear and unbiased information is essential to alleviate the confusion experienced by many consumers and empower them to make informed decisions regarding seafood consumption. As the market changes and more fish originate from aquaculture sources, where diets are controlled, …

Developing Strategies To Mitigate And Control Environmental Listeria Monocytogenes In Retail Delis, Susan Rose Hammons

Open Access Theses

There are many research avenues being investigated at institutions nationwide with the intent to reduce the public health risk of listeriosis. Approaches include studies on L. monocytogenes transmission dynamics in retail delis, observations of deli worker practices, a structured expert elicitation on transmission dynamics and statistical modeling of listeriosis risk impact factors. This thesis describes my contribution to the work being done in our group to (i) understand prevalence and persistence of L. monocytogenes and Listeria species non-monocytogenes on environmental surfaces (food contact and non-food contact); (ii) identify characteristics or practices which place stores at increased risk for L. …

Anticipatory And Reactive Responses To Chocolate Restriction In Frequent Chocolate Consumers, Chelsey L. Keeler

Open Access Theses

Many individuals have difficulty adhering to a weight loss diet. One possible explanation could be that dietary restriction paradoxically contributes to overconsumption. The objective of this study was to examine ingestive behavior under a forced chocolate restriction, with a focus on the anticipatory restriction period and the post-restriction period in frequent chocolate consumers. Fifty-six male (N=18) and female (N=38) high chocolate consumers with high or low cognitive disinhibition aged 27.70 ± 11.09 years with a mean BMI of 25.68± 5.92 kg/m2 participated. Chocolate snacks were provided for the first, second, and sixth week of the study to establish baseline, pre-restriction …

Characteristics And Practices That May Lead To Listeria Monocytogenes Contamination In Retail Delis, Jingjin Wang

Open Access Theses

The foodborne pathogen, Listeria monocytogenes, can contaminate ready-to-eat (RTE) deli meats at retail delis, since retail delis provide an ideal environment for L. monocytogenes growth. Current risk assessment indicates that 83% of listeriosis cases from RTE deli meats are all from RTE deli that are sliced at retail delis. Strategies for controlling L. monocytogenes in retail establishments are essential to prevent post-processing contamination of RTE foods. In order to determine the potential risk factors associated with L. monocytogenes contamination and develop best practices to reduce L. monocytogenes , we sampled the retail establishment environment, isolated L. monocytogenes, and …

Study Of The Sustainbility Issues Of Food Production Using Vertical Farm Methods In An Urban Environment Within The State Of Indiana, Victor Mendez Perez

Open Access Theses

There are several problems around the globe related to water scarcity, energy shortages, deforestation and loss of fertile land, and food security. Climate change is making environmental and social issues arise. Issues such as, changes in the agricultural landscape, increasing population (9 billion is the expected population for 2040), rising food borne illnesses, scarcity of drinking water, and more crop failure due to plant pathogens and insect pests, among other factors make this study specially significant. Vertical farming could be a potencial solution to some of these problems.

Due to these problems the present thesis analyzed vertical farming as a …

Efficacy Of Manual Dishwashing And Sanitizer Wiping In Removing Food Soils From Chel Knives, Xiaodi Sun

Open Access Theses

Improperly cleaned and sanitized chef knives may present a potential contamination risk and thus be a risk for foodborne illness. Therefore, ensuring effective cleaning and sanitization of knife surfaces may help prevent the spread of foodborne pathogens. The primary objective of this research was to compare the efficacies of two different cleaning methods (three-compartment manual dishwashing and sanitizer wiping) at removing food soils from contaminated chef knives using procedures that are typical of restaurant kitchens. Knife wiping and washing procedures were determined and standardized according to an observation of knife cleaning behavior in a kitchen. Adenosine triphosphate (ATP) bioluminescence was …

Effects Of Wheat Grain Moisture: Quality, Germination, And Relationship To Accumulated Growing Degree Days, Kirsten Thomas

Open Access Theses

Bread wheat (Triticum aestivum L.) is a cereal crop of global importance. As global demand increases, it is essential to increase the quality and efficiency of crop production. Harvesting wheat early provides an opportunity for increased grain quality, and it may also allow the grower to double-crop soybean ( Glycine max L.) after wheat more effectively. Our objectives were to determine if harvesting grain early, at high moisture would, 1) increase milling and baking quality and 2) improve germination potential. As a result of these objectives, we will develop a model to predict dry-down of wheat. Five soft red …

Effect Of Carvacrol-Loaded Nanoemulsions On A Bioluminescent Strain Of Escherichia Coli O157:H7, Clara Maria Vasquez Mejia

Open Access Theses

Nanoemulsions have been shown to be effective delivery vehicles for poorly water soluble natural antimicrobials. In this study the antimicrobial activity of carvacrol (5-isopropyl-2-methylphenol) nanoemulsions against a bioluminescent Escherichia coli O157:H7 was investigated using light emission as an indicator of cell viability. Different emulsifiers (Ultralec Lecithin and Tween 20), oils (Palm stearin and Coconut oil) and various carvacrol concentrations (0, 1, 2 and 2.5%) were evaluated. Bioluminescence was monitored in situ using a Hamamatsu (photo multiplier tube) sensor module integrated with a Programmable Logic Controller interfaced with a PC for data acquisition. Bioluminescence decreased rapidly with the addition of emulsions …

Wheat Stored In Triple Layer Plastic Bags: Protection Against The Rice Weevil, Sitophilus Oryzae L. (Coleoptera: Curculionidae) And The Effect Of Air Leaks On Hermetic Storage, Daniel Thomas Martin

Open Access Theses

Triple layer bags consisting of two inner layers of 80 micron thick high-density polyethylene (HDPE) surrounded by an outer woven polypropylene bag have proven to be an effective technology for hermetic storage of cowpea and to prevent loss caused by the cowpea bruchid, Callosobruchus maculatus Fabricius (Coleoptera: Chrysomelidae). This bagging technology is called the Purdue Improved Crop Storage (PICS) system. If PICS bags prove useful for protecting other crops against their insect pests during storage it would allow farmers to store these commodities for longer periods of time to increase personal food availability and market flexibility. Accordingly, PICS bags were …