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Oxidative Stress Protection By Canary Seed (Phalaris Canariensis L.) Peptides In Caco-2 Cells And Caenorhabditis Elegans, Uriel Urbizo-Reyes, Kee-Hong Kim, Lavanya Reddivari, Joseph M. Anderson Jun 2022

Oxidative Stress Protection By Canary Seed (Phalaris Canariensis L.) Peptides In Caco-2 Cells And Caenorhabditis Elegans, Uriel Urbizo-Reyes, Kee-Hong Kim, Lavanya Reddivari, Joseph M. Anderson

Department of Food Science Faculty Publications

During oxidative stress, degenerative diseases such as atherosclerosis, Alzheimer's, and certain cancers are likely to develop. Recent research on canary seed (Phalaris canariensis) peptides has demonstrated the high in vitro antioxidant potential. Thus, this study aimed to assess the cellular and in vivo antioxidant capacity of a low-molecular-weight (<3 kDa) canary seed peptide fraction (CSPF) using Caco-2 cells and the Caenorhabditis elegans model. The results show that the CSPF had no cytotoxicity effect on Caco-2 cells at any tested concentration (0.3-2.5 mg/mL). Additionally, the cellular antioxidant activity (CAA) of the CSPF was concentration-dependent, and the highest activity achieved was 80% by the CSPF at 2.5 mg/mL. Similarly, incubation with …


Intermittent Antibiotic Treatment Accelerated The Development Of Colitis In Il-10 Knockout Mice, Shiyu Li, Yusong Jin, Abigail D. Cox, Dale Lee, Lavanya Reddivari Dec 2021

Intermittent Antibiotic Treatment Accelerated The Development Of Colitis In Il-10 Knockout Mice, Shiyu Li, Yusong Jin, Abigail D. Cox, Dale Lee, Lavanya Reddivari

Department of Food Science Faculty Publications

Many epidemiological studies suggest an association between antibiotic exposure and the development of inflammatory bowel disease [IBD]. However, the majority of these studies are observational and still the question remains, “Does the specific antibiotic administration regimen play a role in the development of colitis?” This study aimed to compare the possible effects of continuous and intermittent antibiotic exposure on the development of colitis using a colitis-susceptible IL-10 knockout [IL-10–/–] mouse model.


Enzyme Kinetics, Molecular Docking, And In Silico Characterization Of Canary Seed (Phalaris Canariensis L.) Peptides With Ace And Pancreatic Lipase Inhibitory Activity, Uriel Urbizo-Reyes, Andrea M. Liceaga, Lavanya Reddivari, Kee-Hong Kim, Joseph M. Anderson Dec 2021

Enzyme Kinetics, Molecular Docking, And In Silico Characterization Of Canary Seed (Phalaris Canariensis L.) Peptides With Ace And Pancreatic Lipase Inhibitory Activity, Uriel Urbizo-Reyes, Andrea M. Liceaga, Lavanya Reddivari, Kee-Hong Kim, Joseph M. Anderson

Department of Food Science Faculty Publications

The bioactivity of canary seed peptides (CSP) towards metabolism-regulating enzymes was evaluated. Peptides with angiotensin-converting enzyme (ACE), dipeptidyl peptidase IV (DPP-IV), and pancreatic lipase activity remained stable (p < 0.05) to simulated gastrointestinal digestion (SGD). CSP-SGD were transported efficiently (>10%) through the Caco-2 monolayer, indicating absorption through the intestinal epithelium. Lineweaver-Burk plots demonstrated that CSP-SGD act as mixed-type inhibitors for DPP-IV and α-glucosidase. Furthermore, CSP-SGD were potent as antihypertensive and antiobesity agents. Molecular docking and in silico analyses were targeted to understand CSP-SGD interactions with ACE and pancreatic lipase. ACE-inhibitory peptides (LHPQ, QTPHQ, KPVPR, and ELHPQ) acted as non-competitive inhibitors by destabilization of the transition state and Zn(II) coordination in …


Clean Label In Bread, Maite Cristina Alava Vargas, Senay Simsek Aug 2021

Clean Label In Bread, Maite Cristina Alava Vargas, Senay Simsek

Department of Food Science Faculty Publications

Bread is considered a staple food worldwide, and therefore there is much interest in research around the topic. The bread industry is usually looking for ways to improve its formulations. Therefore, other ingredients such as dough conditioners, crumb softeners, emulsifiers, and surfactants can be added to enhance bread quality. These ingredients perform functions such as helping standardize processes in the industry, reducing dough-mixing time, increasing water absorption, improving bread quality, and extending its shelf life. Consumers are concerned about the effect of these ingredients on their health, and this has increased the popularity of clean-label bread formulations. A clean label …


Designing A Computer-Vision Application: A Case Study For Hand-Hygiene Assessment In An Open-Room Environment, Chengzang Zhong, Amy R. Reibman, Hansel A. Mina, Amanda J. Deering Aug 2021

Designing A Computer-Vision Application: A Case Study For Hand-Hygiene Assessment In An Open-Room Environment, Chengzang Zhong, Amy R. Reibman, Hansel A. Mina, Amanda J. Deering

Department of Food Science Faculty Publications

Hand-hygiene is a critical component for safe food handling. In this paper, we apply an iterative engineering process to design a hand-hygiene action detection system to improve food- handling safety. We demonstrate the feasibility of a baseline RGB-only convolutional neural network (CNN) in the restricted case of a single scenario; however, since this baseline system performs poorly across scenarios, we also demonstrate the application of two methods to explore potential reasons for its poor performance. This leads to the development of our hierarchical system that incorporates a variety of modalities (RGB, optical flow, hand masks, and human skeleton joints) for …


Effects Of Salts On Structural, Physicochemical And Rheological Properties Of Low-Methoxyl Pectin/Sodium Caseinate Complex, Shengyu Fan, Fang Fang, Ailing Lei, Jiong Zheng, Fusheng Zhang Aug 2021

Effects Of Salts On Structural, Physicochemical And Rheological Properties Of Low-Methoxyl Pectin/Sodium Caseinate Complex, Shengyu Fan, Fang Fang, Ailing Lei, Jiong Zheng, Fusheng Zhang

Department of Food Science Faculty Publications

The addition of salts is an effective way to improve the properties of polysaccharide/protein complexes for use in foods. However, there is no comparative study on the effects of different ions on the complex system of low methoxyl pectin (LMP)/ sodium caseinate (CAS) complex. The effects of different concentrations of three salt ions (Na+, K+, Ca2+) on the physicochemical and rheological properties of the LMP/CAS complex were determined in this study, and the structure of LMP/CAS complex was characterized. The results showed that the addition of these three salt ions affected zeta potential, particle …


In Vitro Antioxidant And Antihypertensive Activity Of Edible Insects Flours (Mealworm And Grasshopper) Fermented With Lactococcus Lactis Strains, Adilene Mendoza-Salazar, Lourdes Santiago-Lopez, Maria J. Torres-Llanez, Adrian Hernandez-Mendoza, Belinda Vallejo-Cordoba, Andrea M. Liceaga, Aaron E. Gonzalez-Cordova Aug 2021

In Vitro Antioxidant And Antihypertensive Activity Of Edible Insects Flours (Mealworm And Grasshopper) Fermented With Lactococcus Lactis Strains, Adilene Mendoza-Salazar, Lourdes Santiago-Lopez, Maria J. Torres-Llanez, Adrian Hernandez-Mendoza, Belinda Vallejo-Cordoba, Andrea M. Liceaga, Aaron E. Gonzalez-Cordova

Department of Food Science Faculty Publications

The objective of the present study was to evaluate the potential antioxidant and angiotensin converting enzyme inhibition (ACEI) activity of edible insect flours fermented with Lactococcus lactis strains. For the fermentation, mealworm and grasshoppers flours were dissolved (0.5% w/v) in buffer solution (pH 7.0) and individually inoculated (3%) with Lactococcus lactis strains (NRRL B-50571, NRRL B-50572). The samples were incubated for 72 h at 30 ◦C, and the pH was recorded. The degree of hydrolysis (DH) and protein content were determined. The total polyphenol compounds, antioxidant activity (ABTS, DPPH, ORAC, and FRAP), and ACEI of the <3 kDa fractions were ana- lyzed. The pH of the fermented samples decreased to 3.5–3.9 (p < 0.05). The fermented grasshopper flour showed an increased DH (0.42%) and overall higher total polyphenol content (8.23 mg Gallic Acid Equivalent/mL). In general, the highest antioxidant activity was for the grasshopper fractions fermented for 24 h by Lactococcus lactis NRRL B-50572, which also showed 23.47% ACEI inhibition with an IC50 of 0.97 mg/mL. The peptide profile obtained increased after fermentation, being higher for the mealworm flour fermented sample. This study presents, for the first time, the use of specific strains of Lactococus lactis for fermenting edible insect-derived products in the production of bioactive compounds with potential antioxidant and antihypertensive activity.


Inulin Fermentable Fiber Ameliorates Type I Diabetes Via Il22 And Short-Chain Fatty Acids In Experimental Models, Jun Zou, Lavanya Reddivari, Zhenda Shi, Shiyu Li, Yanling Wang, Alexis Bretin, Vu L. Ngo, Michael Flythe, Michael Pellizzon, Benoit Chassaing, Andrew T. Gewirtz Jul 2021

Inulin Fermentable Fiber Ameliorates Type I Diabetes Via Il22 And Short-Chain Fatty Acids In Experimental Models, Jun Zou, Lavanya Reddivari, Zhenda Shi, Shiyu Li, Yanling Wang, Alexis Bretin, Vu L. Ngo, Michael Flythe, Michael Pellizzon, Benoit Chassaing, Andrew T. Gewirtz

Department of Food Science Faculty Publications

Nourishment of gut microbiota via consumption of fermentable fiber promotes gut health and guards against metabolic syndrome. In contrast, how dietary fiber impacts type 1 diabetes is less clear.


Complexation With Polysaccharides Enhanced Polyphenol Gastrointestinal Stability And Activity, Wenyi Fu, Shiyu Li, Lavanya Reddivari Jun 2021

Complexation With Polysaccharides Enhanced Polyphenol Gastrointestinal Stability And Activity, Wenyi Fu, Shiyu Li, Lavanya Reddivari

Department of Food Science Faculty Publications

Fruits and vegetables contain dietary polyphenols and polysaccharides. Accumulating evidence suggests that polyphenol- containing whole foods are protective against inflammation-promoted chronic colonic diseases. However, isolated polyphenols are less stable and may not confer the same gastrointestinal health benefits as that of the whole food matrix. Therefore, we hypothesized that the complex- ation of anthocyanins, a class of polypheonols, with polysaccharides would enhance colonic concentration and stability of anthocyanins, and attenuate impaired barrier function.


Improvement Of Gut Barrier Function By Potato Anthocyanins Is Dependent On Gut Microbiota, Shiyu Li, Lavanya Reddivari Jun 2021

Improvement Of Gut Barrier Function By Potato Anthocyanins Is Dependent On Gut Microbiota, Shiyu Li, Lavanya Reddivari

Department of Food Science Faculty Publications

Ulcerative colitis (UC) is characterized by chronic colonic inflammation, impaired barrier function and gut bacterial dysbiosis. Anthocyanin-containing potatoes have been shown to maintain the intestinal barrier function in colitic mice. However, the role of gut microbiota in the anti-colitic effects of anthocyanin- containing potatoes is not clear. This study evaluated the gut barrier protective efficacy of purple- and red-fleshed potatoes using a DSS- induced murine model of colitis with the intact and antibiotic-depleted microbiota.


Insects As A Source Of Phenolic Compounds And Potential Health Benefits, M C. Nino, Lavanya Reddivari, C Osorio, I Kaplan, A M. Liceaga Jun 2021

Insects As A Source Of Phenolic Compounds And Potential Health Benefits, M C. Nino, Lavanya Reddivari, C Osorio, I Kaplan, A M. Liceaga

Department of Food Science Faculty Publications

The use of insects in traditional medicine and unveiling the chemical structure of the bright pigments in butterfly wings led to the discovery of bioactive phenolic compounds in the insect bodies. These metabolites have been found not only due to the insect absorption and metabolisation of the plant-derived phenolic present in their diet, but also from the ability of insects to synthesise phenolic compounds de novo through the sclerotisation process. Plant phenolics are secondary metabolites involved in the protection of tissues against UV radiation, herbivores, and pathogens, as well as pigmentation of fruits and flowers. These bioactive compounds exhibit antioxidant, …


Anthocyanin-Containing Purple Potatoes Ameliorate Dss-Induced Colitis In Mice, Shiyu Li, Tianmin Wang, Baojun Xu, Vijay Indukuri, Jairam Vanamala, Lavanya Reddivari Mar 2021

Anthocyanin-Containing Purple Potatoes Ameliorate Dss-Induced Colitis In Mice, Shiyu Li, Tianmin Wang, Baojun Xu, Vijay Indukuri, Jairam Vanamala, Lavanya Reddivari

Department of Food Science Faculty Publications

Ulcerative colitis (UC), a major form of inflammatory bowel disease (IBD), is on the rise worldwide. Approximately three million people suffer from IBD in the United States alone, but the current therapeutic options (e.g., corticosteroids) come with adverse side effects including reduced ability to fight infections. Thus, there is a critical need for developing effective, safe and evidence-based food products with anti-inflammatory activity. This study evaluated the antiinflammatory potential of purple-fleshed potato using a dextran sodium sulfate (DSS) murine model of colitis. Mice were randomly assigned to control (AIN-93G diet), P15 (15% purple-fleshed potato diet) and P25 (25% purple-fleshed potato …


Characterization Of Maize Near-Isogenic Lines With Enhanced Flavonoid Expression To Be Used As Tools In Diet-Health Complexity, Binning Wu, Haotian Chang, Rich Marini, Surinder Chopra, Lavanya Reddivari Jan 2021

Characterization Of Maize Near-Isogenic Lines With Enhanced Flavonoid Expression To Be Used As Tools In Diet-Health Complexity, Binning Wu, Haotian Chang, Rich Marini, Surinder Chopra, Lavanya Reddivari

Department of Food Science Faculty Publications

Increasing incidence of chronic diseases in the 21st century has emphasized the importance of developing crops with enhanced nutritional value. Plant-based diets are associated with reduced incidence of many chronic diseases. The growing population and increased food demand have prioritized the development of high-yielding commercial crop varieties at the expense of natural flavors as well as health-benefiting compounds including polyphenols. Flavonoids are a large subfamily of polyphenols abundant in the plant kingdom with known health-promoting effects, making them a promising trait to be re-introduced into elite lines. Given the vast array of flavonoids and the complexity of plant food metabolome …


Serum Carotenoids And Pediatric Metabolic Index Predict Insulin Sensitivity In Mexican American Children, Srinivas Mummidi, Vidya S. Farook, Lavanya Reddivari, Joselin Hernandez-Ruiz, Alvaro Diaz-Badillo, Sharon P. Fowler, Roy G. Resendez, Feroz Akhtar, Donna M. Lehman, Cristopher P. Jenkinson, Rector Arya, Jane L. Lynch, Jose A. Canas, Ralph A. Defronzo, Daniel E. Hale, John Blangero, Juan Carlos Lopez-Alvarenga, Ravindranath Duggirala, Jairam K. P. Vanamala Jan 2021

Serum Carotenoids And Pediatric Metabolic Index Predict Insulin Sensitivity In Mexican American Children, Srinivas Mummidi, Vidya S. Farook, Lavanya Reddivari, Joselin Hernandez-Ruiz, Alvaro Diaz-Badillo, Sharon P. Fowler, Roy G. Resendez, Feroz Akhtar, Donna M. Lehman, Cristopher P. Jenkinson, Rector Arya, Jane L. Lynch, Jose A. Canas, Ralph A. Defronzo, Daniel E. Hale, John Blangero, Juan Carlos Lopez-Alvarenga, Ravindranath Duggirala, Jairam K. P. Vanamala

Department of Food Science Faculty Publications

High concentrations of carotenoids are protective against cardiometabolic risk traits (CMTs) in adults and children. We recently showed in non-diabetic Mexican American (MA) children that serum α-carotene and β-carotene are inversely correlated with obesity measures and triglycerides and positively with HDL cholesterol and that they were under strong genetic influences. Additionally, we previously described a Pediatric Metabolic Index (PMI) that helps in the identification of children who are at risk for cardiometabolic diseases. Here, we quantified serum lycopene and β-cryptoxanthin concentrations in approximately 580 children from MA families using an ultraperformance liquid chromatography-photodiode array and determined their heritabilities and correlations …


Characterization Of Maize Near-Isogenic Lines With Enhanced Flavonoid Expression To Be Used As Tools In Diet-Health Complexity, Binning Wu, Haotian Chang, Rich Marini, Surinder Chopra, Lavanya Reddivari Jan 2021

Characterization Of Maize Near-Isogenic Lines With Enhanced Flavonoid Expression To Be Used As Tools In Diet-Health Complexity, Binning Wu, Haotian Chang, Rich Marini, Surinder Chopra, Lavanya Reddivari

Department of Food Science Faculty Publications

Increasing incidence of chronic diseases in the 21st century has emphasized the importance of developing crops with enhanced nutritional value. Plant-based diets are associated with reduced incidence of many chronic diseases. The growing population and increased food demand have prioritized the development of high-yielding commercial crop varieties at the expense of natural flavors as well as health-benefiting compounds including polyphenols. Flavonoids are a large subfamily of polyphenols abundant in the plant kingdom with known health-promoting effects, making them a promising trait to be re-introduced into elite lines. Given the vast array of flavonoids and the complexity of plant food metabolome …


Identification And Characterization Of Edible Cricket Peptides On Hypertensive And Glycemic In Vitro Inhibition And Their Anti-Inflammatory Activity On Raw 264.7 Macrophage Cells, Felicia Hall, Lavanya Reddivari, Andrea M. Liceaga Nov 2020

Identification And Characterization Of Edible Cricket Peptides On Hypertensive And Glycemic In Vitro Inhibition And Their Anti-Inflammatory Activity On Raw 264.7 Macrophage Cells, Felicia Hall, Lavanya Reddivari, Andrea M. Liceaga

Department of Food Science Faculty Publications

Recent studies continue to demonstrate the potential of edible insects as a protein base to obtain bioactive peptides applicable for functional food development. This study aimed at identifying antihypertensive, anti-glycemic, and anti-inflammatory peptides derived from the in vitro gastrointestinal digests of cricket protein hydrolysates. After sequential fractionation, the protein digest subfraction containing the lowest molecular weight (<0.5 kDa), hydrophobic (C18) and cationic peptides (IEX) was found responsible for the most bioactivity. The cationic peptide fraction significantly reduced (p < 0.05) α-amylase, α-glucosidase, and angiotensin converting enzyme (ACE) activity in vitro, and also inhibited the expression of NF-κB in RAW 264.7 macrophage cells. A total of 28 peptides were identified with mass spectrometry (LC–MS/MS) and de novo sequencing from the potent fraction. Three novel peptides YKPRP, PHGAP, and VGPPQ were chosen for the molecular docking studies. PHGAP and VGPPQ exhibited a higher degree of non-covalent interactions with the enzyme active site residues and binding energies comparable to captopril. Results from this study demonstrate the bioactive potential of edible cricket peptides, especially as ACE inhibitors.


Role Of Gut Microbiota In Anti-Colitic Effects Of Color-Fleshed Potatoes, Lavanya Reddivari, Shiyu Li, Tianmin Wang, Mary Kennett, Jairam Vanamala Jun 2020

Role Of Gut Microbiota In Anti-Colitic Effects Of Color-Fleshed Potatoes, Lavanya Reddivari, Shiyu Li, Tianmin Wang, Mary Kennett, Jairam Vanamala

Department of Food Science Faculty Publications

The prevalence of ulcerative colitis (UC), a chronic inflammatory bowel disease, is on the rise with ∼700,000 patients in the US alone in 2018. Gut bacterial dysbiosis plays an important role in ulcerative colitis. We have recently shown that anthocyanin-containing potatoes exert anti-inflammatory activity in colitic mice. However, no information is available on whether gut bacteria play a role in the anti-colitic activity of color-fleshed potatoes. This study examined the anti-colitic activity of red/purple-fleshed potatoes in mice with intact and antibiotic-ablated microbiome.


Maize Flavan-4-Ols And Anthocyanins Alleviated Dextran Sulfate Sodium-Induced Colitis In Mice Via Intestinal Barrier Function Restoration, Binning Wu, Shiyu Li, Haotian Chang, Ruth Anderson, Surinder Chopra, Lavanya Reddivari May 2020

Maize Flavan-4-Ols And Anthocyanins Alleviated Dextran Sulfate Sodium-Induced Colitis In Mice Via Intestinal Barrier Function Restoration, Binning Wu, Shiyu Li, Haotian Chang, Ruth Anderson, Surinder Chopra, Lavanya Reddivari

Department of Food Science Faculty Publications

This study investigated the anti-inflammatory effects of anthocyanins and flavan-4-ols (precursor of phlobaphenes) within a whole-food matrix against dextran sulfate sodium (DSS)-induced colitis using four maize near-isogenic lines (NILs) that differ only in a single class of flavonoids. The four NILs are A (lacks anthocyanins and phlobaphenes), B (phlobaphenes +), C (anthocyanins +) and D (anthocyanins + and phlobaphenes +).


A Swine Model Of Soy Protein-Induced Food Allergy: Implications In Human And Swine Nutrition, John Scott Radcliffe, Luiz F. Brito, Lavanya Reddivari, Monica Schmidt, Eliont M. Herman, Allan P. Schinckel Jun 2019

A Swine Model Of Soy Protein-Induced Food Allergy: Implications In Human And Swine Nutrition, John Scott Radcliffe, Luiz F. Brito, Lavanya Reddivari, Monica Schmidt, Eliont M. Herman, Allan P. Schinckel

Department of Food Science Faculty Publications

There is extensive literature on the development and management of food allergy, but some basic questions such as why some proteins are allergenic while closely related proteins from other species are not allergenic, remain to be answered. This paper is not a detailed review of food allergies, but rather an overview of current knowledge regarding soy food allergies, with a focus on the pig as a model for the study of soy allergies and with additional information on how soy allergies and use of soybean-sourced feed can affect production animal agriculture.


The Anti-Inflammatory Effects Of Dietary Anthocyanins Against Ulcerative Colitis, Shiyu Li, Binning Wu, Wenyi Fu, Lavanya Reddivari May 2019

The Anti-Inflammatory Effects Of Dietary Anthocyanins Against Ulcerative Colitis, Shiyu Li, Binning Wu, Wenyi Fu, Lavanya Reddivari

Department of Food Science Faculty Publications

Ulcerative colitis (UC), which is a major form of inflammatory bowel disease (IBD), is a chronic relapsing disorder of the gastrointestinal tract affecting millions of people worldwide. Alternative natural therapies, including dietary changes, are being investigated to manage or treat UC since current treatment options have serious negative side effects. There is growing evidence from animal studies and human clinical trials that diets rich in anthocyanins, which are pigments in fruits and vegetables, protect against inflammation and increased gut permeability as well as improve colon health through their ability to alter bacterial metabolism and the microbial milieu within the intestines. …


A Theoretical Model Of Underground Dipole Antennas For Communications In Internet Of Underground Things, Abdul Salam, Mehmet C. Vuran, Xin Dong, Christos Argyropoulos, Suat Irmak Feb 2019

A Theoretical Model Of Underground Dipole Antennas For Communications In Internet Of Underground Things, Abdul Salam, Mehmet C. Vuran, Xin Dong, Christos Argyropoulos, Suat Irmak

Faculty Publications

The realization of Internet of Underground Things (IOUT) relies on the establishment of reliable communication links, where the antenna becomes a major design component due to the significant impacts of soil. In this paper, a theoretical model is developed to capture the impacts of change of soil moisture on the return loss, resonant frequency, and bandwidth of a buried dipole antenna. Experiments are conducted in silty clay loam, sandy, and silt loam soil, to characterize the effects of soil, in an indoor testbed and field testbeds. It is shown that at subsurface burial depths (0.1-0.4m), change in soil moisture impacts …


Dataset Of Water Activity Measurements Of Alcohol: Water Solutions Using A Tunable Diode Laser, Matthew Allan, Lisa Mauer Apr 2017

Dataset Of Water Activity Measurements Of Alcohol: Water Solutions Using A Tunable Diode Laser, Matthew Allan, Lisa Mauer

Department of Food Science Faculty Publications

The data presented in this article are related to the research article entitled “RH-temperature phase diagrams of hydrate forming deliquescent crystalline ingredients” (Allan and Mauer, 2017) [1]. The data are water activity measurements of alcohol:water solu- tions (methanol:water and ethanol:water solutions at varying molar ratios) at different temperatures collected using the Tunable Diode Laser by Decagon Devices. The measured water activities of ethanol:water solutions were correlated to the initial volumetric ratios to produce polynomial equations that can be used to cal- culate the needed initial volumetric ratios for water activity con- trolled solutions. The data sets and polynomial equations are …


Fructose 1,6-Bisphosphate Aldolase, A Novel Immunogenic Surface Protein On Listeria Species, Mercelo L. Mendonça, Gustavo Marçal Schmidt Garcia Moreira, Fabrício Fabrício Conceição, Michael Hust, Karla Sequeira Mendonça, Ângela Nunes Moreira, Rodrigo Correa Orrea França, Wladimir Padilha Da Silva, Arun K. Bhunia, José Antônio Guimarães Uimarães Aleixo Aug 2016

Fructose 1,6-Bisphosphate Aldolase, A Novel Immunogenic Surface Protein On Listeria Species, Mercelo L. Mendonça, Gustavo Marçal Schmidt Garcia Moreira, Fabrício Fabrício Conceição, Michael Hust, Karla Sequeira Mendonça, Ângela Nunes Moreira, Rodrigo Correa Orrea França, Wladimir Padilha Da Silva, Arun K. Bhunia, José Antônio Guimarães Uimarães Aleixo

Department of Food Science Faculty Publications

Listeria monocytogenes is a ubiquitous food-borne pathogen, and its presence in food or production facilities highlights the importance of surveillance. Increased understanding of the surface exposed antigens on Listeria would provide potential diagnostic and therapeutic targets. In the present work, using mass spectrometry and genetic cloning, we show that fructose-1,6-bisphosphate aldolase (FBA) class II in Listeria species is the antigen target of the previously described mAb-3F8. Western and dot blot assays confirmed that the mAb-3F8 could distinguish all tested Listeria species from close-related bacteria. Localization studies indicated that FBA is present in every fraction of Listeria cells, including supernatant and …