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Study On Immobilization Of Wheat Bran Esterase With Fibre Membrance, Ye Lin, Jiang Lu, Shen Guanghui, Li Shanshan, Wu Hejun, Zhang Zhiqing
Study On Immobilization Of Wheat Bran Esterase With Fibre Membrance, Ye Lin, Jiang Lu, Shen Guanghui, Li Shanshan, Wu Hejun, Zhang Zhiqing
Food and Machinery
Taking the plant-esterase from wheat bran as the enzyme source, comparing the physical adsorption method and the cross-linking with adsorption methods, and the glass fiber film as adsorption carrier, and then exploreed the optimum conditions for immobilization use enzyme activity recovery as the evaluation index. On the basis of single-factors experiments, the Box-behnken center-united experimental design principles were used to design the experiment and the response surface analysis of 3 factors (the buffer’s pH, immobilized time and immobilized temperature) and the interactive effect on immobilization. The optimum immobilized conditions were confirmed as followed: phosphate buffer’s pH 7.0, immobilized time 4 …