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Articles 1 - 5 of 5
Full-Text Articles in Life Sciences
Surface Moisture And Osmotic Stress As Factors That Affect The Sanitizing Of Beef Tissue Surfaces, James S. Dickson
Surface Moisture And Osmotic Stress As Factors That Affect The Sanitizing Of Beef Tissue Surfaces, James S. Dickson
James S. Dickson
The use of osmotic stress or desiccation in combination with 2% acetic acid reduced the numbers of Salmonella typhimurium or Listeria monocytogenes on beef tissue to a greater degree than the acid alone. Bacteria were osmotically stressed by NaCl, CaCl2, or sucrose solutions. Pre-washing with 20% NaCl followed by sanitizing with acetic acid reduced the numbers of S. typhimurium an additional 1.5-to 2-log cycles compared with acetic acid alone. Similar reductions were seen by allowing the tissue surfaces to dry before the application of the acid. Desiccation in combination with acetic acid was also effective in reducing the numbers of …
Purification And Immunological Reactivity Of Commercial Microbial Milk Clotting Enzyme Preparations, Chima I. Osuala
Purification And Immunological Reactivity Of Commercial Microbial Milk Clotting Enzyme Preparations, Chima I. Osuala
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Commercial microbial milk clotting enzyme preparations were purified by immunoaffinity chromatography using purified antibody covalently coupled to porous glass beads as the column matrix. Commercial enzyme preparation diluted in 1 mM sodium acetate buffer at pH 5.0 was then biospecifically adsorbed to the column matrix by end-over-end mixing of the glass-antibody complex in the enzyme solution for 12 h at 5°C. The antibody bound enzyme adsorbed glass beads were soaked in .2 M glycine or ethanolarnine at pH 7.0 to block uncoupled reactive sites on the matrix. Following this, the column was washed with 1 mM sodium acetate …
Transfer Of Listeria Monocytogenes And Salmonella Typhimurium Between Beef Tissue Surfaces, James S. Dickson
Transfer Of Listeria Monocytogenes And Salmonella Typhimurium Between Beef Tissue Surfaces, James S. Dickson
James S. Dickson
Beef tissue surfaces were inoculated with either Listeria monocytogenes or Salmonella typhimurium and placed in contact with uninoculated tissue surfaces. The transfer of bacteria from one surface to another was determined under conditions designed to simulate contamination during meat processing. Bacterial transfer decreased when the initial inoculum was allowed to adsorb to the base tissue prior to contact with the second tissue. The type of base tissue was a factor (P<0.05), with a greater transfer from fat with contact times of less than 1 min and a greater transfer from lean with longer contact times.
Comparison Of Methods For Purification Of Dna From Rice, Theresa L. Hines, Neela R. Patel, Daoud Abu-Diab, Michael Sikes, Alvan K. Karlin
Comparison Of Methods For Purification Of Dna From Rice, Theresa L. Hines, Neela R. Patel, Daoud Abu-Diab, Michael Sikes, Alvan K. Karlin
Journal of the Arkansas Academy of Science
Three techniques were employed to purify genomic DNA from deomstic rice (Oryza sativa L). Following extraction, the DNA was electrophoresed through agarose to determine its integrity. We determined that spooling yielded better quality, through lower quantity DNA than either of the other two techniques.
Screening Rice (Oryza Sativa L.) Genotypes For Drought Tolerance Under Field Conditions, Mazo Price, Md. Jalaluddin, R. H. Dilday
Screening Rice (Oryza Sativa L.) Genotypes For Drought Tolerance Under Field Conditions, Mazo Price, Md. Jalaluddin, R. H. Dilday
Journal of the Arkansas Academy of Science
We evaluated the root pulling resistance (RPR) technique developed at the International Rice Research Institute (IRRI) for transplanted rice (Oryza sativa L.) to determine its applicability for assessing the drought tolerance of direct seeded rice. Experiments were conducted in 1988 and 1989 at the University of Arkansas at Pine Bluff Agricultural Research Farm. Fifty genotypes from four countries were grown with and without irrigation. The genotypes identified as drought tolerant germplasm by the RPR method in both years were significantly correlated. In both 1988 and 1 989, RPR was directly related to maximum root length, root number, and root dry …