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Distribution And Diversity Of Bacterial Chemolithotrophs In Marine And Freshwater Sediments, Lisa M. Nigro
Distribution And Diversity Of Bacterial Chemolithotrophs In Marine And Freshwater Sediments, Lisa M. Nigro
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Bacterial chemolithotroph population structure has been investigated in Lowes Cove marine intertidal mudflat and Damariscotta Lake, Maine. A 492 to 495 fragment of the cbbL gene, coding for the large subunit of Form I ribulose-1,5- bisphosphate carboxylase/oxygenase (rubisco) was amplified from lake surface (upper 2 mm) sediments and mudflat surface (upper 2 mm), subsurface (5-7 cm), and Mya arenaria burrow wall sediments, as well as sulfide-oxidizing bacterial mat samples. Amplified DNA was used to construct cbbL clone libraries. Phylogenetic analysis showed that Damariscotta Lake cbbL clones were mainly of the 1C type, indicating a facultative carbon monoxide/hydrogen-oxidizing community. Conversely, clones …