Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

Evaluation Of Automated Threshold Selection Methods For Accurately Sizing Microscopic Fluorescent Cells By Image-Analysis, Me Sieracki, Se Reichenbach, Kl Webb Nov 1989

Evaluation Of Automated Threshold Selection Methods For Accurately Sizing Microscopic Fluorescent Cells By Image-Analysis, Me Sieracki, Se Reichenbach, Kl Webb

VIMS Articles

The accurate measurement of bacterial and protistan cell biomass is necessary for understanding their population and trophic dynamics in nature. Direct measurement of fluorescently stained cells is often the method of choice. The tedium of making such measurements visually on the large numbers of cells required has prompted the use of automatic image analysis for this purpose. Accurate measurements by image analysis require an accurate, reliable method of segmenting the image, that is, distinguishing the brightly fluorescing cells from a dark background. This is commonly done by visually choosing a threshold intensity value which most closely coincides with the outline …


Electrostatic Effects On Airborne Asbestos Monitoring, Roxanne Francis Oct 1989

Electrostatic Effects On Airborne Asbestos Monitoring, Roxanne Francis

Biological Sciences Theses & Dissertations

The affects of electrostatic forces on the Membrane Filter Method (MFM) used for air-borne asbestos monitoring have been investigated for many years. Several studies have indicated that these forces interfere with the collection of asbestos fibers on the membrane filter, which results in an underestimated analysis of the airborne concentration. By varying the electroconductivity of the extension cowls on the filter cassettes, it was speculated that the significance of the electrostatic forces could be statistically analyzed. Four sets of filter cassettes with grounded conductive (GC) extension cowls, ungrounded conductive (UC) extension cowls, grounded nonconductive (GN) extension cowls, and ungrounded nonconductive …