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Full-Text Articles in Life Sciences
Metabolic Regulation During Extraembryonic Endoderm Differentiation, Mohamed Gatie
Metabolic Regulation During Extraembryonic Endoderm Differentiation, Mohamed Gatie
Electronic Thesis and Dissertation Repository
The inner cell mass undergoes orchestrated cellular divisions resulting in the formation of the epiblast (EPI) and primitive endoderm (PrE). Understanding the process of cell fate specification is crucial to appreciate the intricacies of proper embryonic development. While the mouse embryo is an excellent model, limitations do exist with number, technical challenges, and accessibility, therefore, in my thesis I employed two cell-based models to recapitulate the EPI-PrE fate in vitro. Many signaling pathways have been implicated in this lineage decision, metabolism and its downstream products have been recently regarded as a driver of lineage commitment. Using various biochemical, molecular, …
Characterizing The Effects Of Pyrroloquinoline Quinone (Pqq) Supplementation On Skeletal Muscle Mitochondrial Function And Myogenesis During Oxidative Stress And Iugr., Allyson J. Wood
Electronic Thesis and Dissertation Repository
Intrauterine growth restriction (IUGR) affects 10-15% of births and is associated with placental insufficiency (PI), resulting in fetal oxidative stress (OS). This OS is a factor in the predisposition to postnatal noncommunicable disease (NCD) of which muscle mitochondrial dysfunctional is a key aspect. Pyrroloquinoline quinone (PQQ), an antioxidant-like compound, is capable of OS reduction and promotes mitochondrial function, though limited research has focused on its effects in in utero skeletal muscle. This study sought to investigate the impact of in vitro H2O2, a model of OS, and an in vivo model of OS associated IUGR, with …
Phenotypic And Metabolic Plasticity In Canine Cellular Reprogramming, Ian C. Tobias
Phenotypic And Metabolic Plasticity In Canine Cellular Reprogramming, Ian C. Tobias
Electronic Thesis and Dissertation Repository
Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) derived from large mammals reproduce few characteristics displayed by rodent or human counterparts. This is complicated by the inherent plasticity of mammalian ESC/iPSC cultures that resemble a variety of developmental stages including the naïve and primed pluripotent states. Defining the extrinsic signals that modulate the developmental identity of canine ESC/iPSC (i.e. primed versus naïve) will improve knowledge integration with more sophisticated rodent and primate research. In this thesis, I sought to determine if manipulation of the culture environment can promote nuclear and metabolic reprogramming of canine cell lines towards a …