Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
The C. Elegans Heterochronic Gene Lin-4 Encodes Small Rnas With Antisense Complementarity To Lin-14, Rosalind Lee, Rhonda Feinbaum, Victor Ambros
The C. Elegans Heterochronic Gene Lin-4 Encodes Small Rnas With Antisense Complementarity To Lin-14, Rosalind Lee, Rhonda Feinbaum, Victor Ambros
Victor R. Ambros
lin-4 is essential for the normal temporal control of diverse postembryonic developmental events in C. elegans. lin-4 acts by negatively regulating the level of LIN-14 protein, creating a temporal decrease in LIN-14 protein starting in the first larval stage (L1). We have cloned the C. elegans lin-4 locus by chromosomal walking and transformation rescue. We used the C. elegans clone to isolate the gene from three other Caenorhabditis species; all four Caenorhabditis clones functionally rescue the lin-4 null allele of C. elegans. Comparison of the lin-4 genomic sequence from these four species and site-directed mutagenesis of potential open reading frames …