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Articles 1 - 30 of 44
Full-Text Articles in Life Sciences
Strategic Nutritional Interventions With Probiotics And Their Relationship To Performance, Feeding Behavior, And Reticulorumen Environment In Cows And Calves Under Subacute Ruminal Acidosis Risk, Gustavo Mazon Correa Alves
Strategic Nutritional Interventions With Probiotics And Their Relationship To Performance, Feeding Behavior, And Reticulorumen Environment In Cows And Calves Under Subacute Ruminal Acidosis Risk, Gustavo Mazon Correa Alves
Theses and Dissertations--Animal and Food Sciences
Subacute ruminal acidosis (SARA) is a digestive disorder in ruminants characterized by extended periods of low reticulorumen pH. This digestive disorder is commonly observed in ruminants fed diets with elevated proportions of non-fibrous carbohydrates. This disorder has been vastly studied in adult dairy cattle and has been associated with losses in milk production, changes in feeding behavior, damage to the gastrointestinal tract, and premature culling. Although vastly studied in adult cattle, there is limited research on the effects of SARA in calves. SARA might be prevented by modifying the reticulorumen environment with probiotic supplements containing lactateutilizing bacteria such as Megasphaera …
Personality Traits Of Dairy Calves And Their Relationship With Feeding Behavior, Activity, And Performance, Emily A. Michalski
Personality Traits Of Dairy Calves And Their Relationship With Feeding Behavior, Activity, And Performance, Emily A. Michalski
Theses and Dissertations--Animal and Food Sciences
Detection of individual differences in personality traits of animals may prove beneficial for producers to help tailor management for individuals and to make selection decisions. Therefore, the objectives of this thesis were to
1) review and evaluate literature on relationships between individual cattle personality traits and feeding behavior, activity, and performance; and
2) evaluate and develop tests suitable for characterizing crossbred dairy-beef calves’ personalities and associations with behavioral patterns and performance.
The existing literature indicates that growth, intake, activity, and milk production measures from precision technologies have associations with cattle personality traits and behaviors identifiable through standardized tests. This indicates …
Utilizing Precision Technologies To Validate A Real-Time Location System For Dairy Cattle And Monitor Calf Behaviors During Heat Stress, Emily Rice
Theses and Dissertations--Animal and Food Sciences
With the increase in on-farm precision dairy technologies (PDT) utilization, large quantities of information are readily available to producers. A more recently available technology for use in livestock species is the real-time location system. These technologies offer dairy producers the opportunity to monitor and track real-time locations of cows, track locomotion patterns, and summarize specific area usage. However, the usefulness of these insights is heavily dependent on the performance of the technology. Therefore, the first objective of this dissertation was to assess the positioning recording performance and the usefulness of the data recorded of a real-time location system (Smartbow GmbH; …
Precision Livestock Farming And Personality As Tools To Understand Dairy Calf Individual Variation In Performance, Behavior, And Response To Stress, Megan Marie Woodrum Setser
Precision Livestock Farming And Personality As Tools To Understand Dairy Calf Individual Variation In Performance, Behavior, And Response To Stress, Megan Marie Woodrum Setser
Theses and Dissertations--Animal and Food Sciences
Individual differences impact animal responses to their environments and can be observed with animal personality (stable behavioral differences with populations) and food neophobia (avoidance of unfamiliar feeds). These individual differences can influence cattle performance and welfare. Precision technologies, such as automatic feeding systems (AFS) and accelerometers continuously measure feeding behaviors and activity and may allow us to relate individual differences to behaviors in the home environment. Therefore, the first objective was to determine if food neophobia was associated with feeding behaviors measured by AFS. Food neophobia was associated with rewarded visits to the AFS, but to no other feeding behaviors. …
Isolation Box Test (Ibt) And Dairy Calf Personality Traits Relationship With Performance Through Weaning, Megan Marie Woodrum Setser
Isolation Box Test (Ibt) And Dairy Calf Personality Traits Relationship With Performance Through Weaning, Megan Marie Woodrum Setser
Theses and Dissertations--Animal and Food Sciences
Personality tests are utilized to characterize individual differences in personality traits across species. Currently, there is no objective personality test that can be implemented on-farm to measure personality in dairy calves. Measuring personality of youngstock in the dairy industry may allow producers to utilize information on individual differences for management decisions and limit the investment in unfit individuals. The isolation box test is a personality test that has potential to be adapted and utilized for dairy calves. The main objective of this thesis is to develop, validate, and utilize an isolation box test for use on-farm in dairy calves. More …
Incorporation Of Summer Annual Mixtures Into Grazing Systems In Kentucky, Kelly Marie Mercier
Incorporation Of Summer Annual Mixtures Into Grazing Systems In Kentucky, Kelly Marie Mercier
Theses and Dissertations--Plant and Soil Sciences
Utilizing summer annual grass-legume forage mixtures has the potential to improve forage yield and nutritive characteristics, and/or animal performance during times when cool-season pasture growth is limited by high temperatures. Legumes can utilize atmospheric nitrogen, which can increase crude protein and forage digestibility in mixtures. As nitrogen application generally improves both the yield and nutritive characteristics of summer annual forages, but can have a negative effect on legume competitiveness, nitrogen fertilizer recommendations for legume-containing summer annual mixtures are not well established.
Two experiments were conducted to determine the feasibility of utilizing summer annual mixtures in Kentucky, USA. The first experiment …
Microbial Interactions Between Compost Bedded Pack Barn Bedding And Teat Exposure In Transition Dairy Cattle, Tanya Lynn France
Microbial Interactions Between Compost Bedded Pack Barn Bedding And Teat Exposure In Transition Dairy Cattle, Tanya Lynn France
Theses and Dissertations--Animal and Food Sciences
Compost bedded pack barns utilize composting methods which provide a soft surface for dairy cows to lie on. This requires optimal microbial growth, which may increase the exposure of mastitis-causing pathogens to the teats of early lactation animals. Bedding characteristics, bedding bacteria, and bacterial counts on the teat skin, teat ends, and in the milk of early lactation dairy cows housed on a compost bedded pack were assessed over a 6-month time. The main objective was to determine the relationship between environmental effects (bedding characteristics and weather conditions) and cow-level (teat skin, teat end, milk) bacteria counts over time in …
The Economics Of Milk Quality Management Practices And Milk Pricing In Simulated United States Dairy Herds, Derek Thomas Nolan
The Economics Of Milk Quality Management Practices And Milk Pricing In Simulated United States Dairy Herds, Derek Thomas Nolan
Theses and Dissertations--Animal and Food Sciences
Mastitis is considered one of the most common and costly diseases in the dairy industry. Intramammary infection status at a herd level is measured using somatic cell count (SCC). Understanding the total cost of an elevated somatic cell count can help influence dairy farmers to lower SCC and select management practices to produce higher quality milk. The first objective of our research was to determine if the cost of an elevated somatic cell count to farms can be decreased through the adoption of management practices with varying expenses. Using stochastic simulation modeling, the adoption of three differently priced management practices …
Validation Of A Commercial Automated Body Condition Scoring System On A Commercial Dairy Farm, Israel L. Mullins, Carissa M. Truman, Magnus R. Campler, Jeffrey M. Bewley, Joao H. C. Costa
Validation Of A Commercial Automated Body Condition Scoring System On A Commercial Dairy Farm, Israel L. Mullins, Carissa M. Truman, Magnus R. Campler, Jeffrey M. Bewley, Joao H. C. Costa
Animal and Food Sciences Faculty Publications
Body condition scoring (BCS) is the management practice of assessing body reserves of individual animals by visual or tactile estimation of subcutaneous fat and muscle. Both high and low BCS can negatively impact milk production, disease, and reproduction. Visual or tactile estimation of subcutaneous fat reserves in dairy cattle relies on their body shape or thickness of fat layers and muscle on key areas of the body. Although manual BCS has proven beneficial, consistent qualitative scoring can be difficult to implement. The desirable BCS range for dairy cows varies within lactation and should be monitored at multiple time points throughout …
Effects Of Yeast-Derived Microbial Protein On Transition Dairy Cow Health And Performance, Gustavo Mazon Correa Alves
Effects Of Yeast-Derived Microbial Protein On Transition Dairy Cow Health And Performance, Gustavo Mazon Correa Alves
Theses and Dissertations--Animal and Food Sciences
The transition period for dairy cows is defined as the three weeks pre and postpartum. During the transition period, dairy cows experience a myriad of metabolic, managerial, and nutritional requirement changes. These changes lead to stress and increased susceptibility to diseases which can negatively affect lactational performance in the short and long term. However, dietary amino acid availability can have a dramatic impact on the health and performance of dairy cows around parturition. Thus, the objective of the thesis was to evaluate the effects of supplementing yeast-derived microbial protein, as an alternative protein source for dairy cows during the transition …
Using An Implantable Microchip For Measuring Body Temperature In Dairy Calves, Megan Woodrum, Melissa Cantor, Joao H. C. Costa
Using An Implantable Microchip For Measuring Body Temperature In Dairy Calves, Megan Woodrum, Melissa Cantor, Joao H. C. Costa
Posters-at-the-Capitol Presentations
Body temperature is frequently used as a method for determining if illness is present with fever detection. Taking rectal temperature or alternatively tympanic temperature can be time consuming and requires restraint of calves. Alternatives, such as implantable microchips that can be passively read using a radio frequency identification (RFID) scanner may allow for easier monitoring of body temperature.
This study aim was to validate an implantable microchip and to determine the best implant site.
Automated Body Condition Scoring: Progression Across Lactation And Its Association With Disease And Reproduction In Dairy Cattle, Carissa Marie Truman
Automated Body Condition Scoring: Progression Across Lactation And Its Association With Disease And Reproduction In Dairy Cattle, Carissa Marie Truman
Theses and Dissertations--Animal and Food Sciences
Body condition scoring is a technique used to noninvasively assess fat reserves. It provides an objective estimate to describe the current and past nutritional status of the dairy cow and has been associated with increased disease risk and breeding success. Traditionally body condition scores are taken manually by visual appraisal on a 1 to 5 scale, in one-quarter increments. However, recent studies have shown the potential of automating the body condition scoring of cows using images. The first objective was to estimate the likelihood of disease development and breeding success, using odds ratios, associated with body condition score scored automatically …
Validation Of An Automated Behavior Monitoring Collar, And Evaluation Of Heat Stress On Lactating Dairy Cow Behavior With Access To A Free Choice Soaker, Lori Nicole Grinter
Validation Of An Automated Behavior Monitoring Collar, And Evaluation Of Heat Stress On Lactating Dairy Cow Behavior With Access To A Free Choice Soaker, Lori Nicole Grinter
Theses and Dissertations--Animal and Food Sciences
Precision dairy technologies (PDT) are becoming more accessible and are therefore becoming more common on commercial dairy farms and in dairy research. Prior to any use of PDT, one should understand the precision, accuracy and bias of the device by a validations studies before interpreting the behavior measurements. Thus, the objective of the first section of my thesis is to validate ruminating, feeding and resting measurements of a behavior monitoring collar used in the second section. Precision dairy technology is used in heat stress studies to compare behavior of cows exposed to different heat stress treatments or abatement strategies. Heat …
Evaluating Costs Associated With Management Decisions Of Replacement Dairy Heifers And Their Impact On The Total Rearing Investment, Anna Catherine Hawkins
Evaluating Costs Associated With Management Decisions Of Replacement Dairy Heifers And Their Impact On The Total Rearing Investment, Anna Catherine Hawkins
Theses and Dissertations--Animal and Food Sciences
Replacement heifer rearing is critical for the future of the dairy operation, especially to improve genetic merit and maintain herd size. A replacement heifer from the day she is born to the day she calves herself is generally a 2-year investment without potential income. A myriad of options exists on how to manage, fed, and ultimately raise replacement heifers. This study quantifies the costs associated with replacement heifer management decisions from birth to calving related to housing, labor, feed and health. The heifer rearing period can be broken into pre and post weaning sections to allow for more understanding the …
The Effects Of Housing On Dairy Cow Comfort, Immune Function, Stress, Productivity, And Milk Quality, Matthew Richard Borchers
The Effects Of Housing On Dairy Cow Comfort, Immune Function, Stress, Productivity, And Milk Quality, Matthew Richard Borchers
Theses and Dissertations--Animal and Food Sciences
Mastitis and milk quality affect every dairy farmer across the globe. Sand bedded freestalls are the industry standard for cow comfort, welfare, and the control of environmental mastitis. Compost bedded packs may be a viable alternative to the sand bedded freestall.
Compost bedded packs are maintained at a consistent level of moisture, nutrients, and aeration to favor compost microorganisms. Greater bacteria counts in bedding have traditionally been associated with increased mastitis rates and mastitis pathogens can be found in the pack and on the teats of cattle housed in even well managed compost bedded pack barns. In spite of this, …
Dairy Cattle Hoof Disease Costs And Considerations For Prevention, Karmella A. Dolecheck
Dairy Cattle Hoof Disease Costs And Considerations For Prevention, Karmella A. Dolecheck
Theses and Dissertations--Animal and Food Sciences
Lameness is considered one of the most important health and welfare issues in the dairy industry. Understanding the total cost per case of disease-specific lameness can help producers select better treatment, prevention, and control strategies for their herds. The first objective of our research was to calculate the costs associated with 3 lameness causing hoof diseases: digital dermatitis, sole ulcer, and white line disease. To accomplish this, a survey of hoof health professionals (hoof trimmers and veterinarians) was conducted to identify treatment related expenditures per case. Data from the hoof trimmer responses to the survey and previously published research were …
Comparing Dairy Farm Performance And Heat Stress Abatment Strategies In The United States Using Summer To Winter Ratios, Jenna M. Guinn
Comparing Dairy Farm Performance And Heat Stress Abatment Strategies In The United States Using Summer To Winter Ratios, Jenna M. Guinn
Theses and Dissertations--Animal and Food Sciences
Heat stress abatement is a challenge for producers in the United States, especially in the southern states. Dairy producers could benefit by having a simply metric to measure heat stress abatement strategies with the goal of motivating improvement in heat stress management. Managing heat stress is key to ameliorating the effects on dairy cow performance. A study was performed to explore the use of a heat stress metric called the Summer to Winter performance ratio (S:W ratio), to quantify and compare farm performance variables among regions of the United States. Summer to Winter ratios were closest to 1.0 in the …
An Evaluation Of Physiological And Behavioral Indicators Of Postpartum Diseases And Heat Stress In Dairy Cows, Amanda Renee Lee
An Evaluation Of Physiological And Behavioral Indicators Of Postpartum Diseases And Heat Stress In Dairy Cows, Amanda Renee Lee
Theses and Dissertations--Animal and Food Sciences
Precision dairy monitoring technologies can be used to monitor changes in physiology and behavior associated with transition period postpartum diseases and heat stress. Research Objective One was to evaluate how traditional visual examination, body condition, and locomotion with and without blood, milk, and urine variables and precision dairy monitoring technologies determine variable association with hyperketonemia, metritis, mastitis, hypocalcemia and retained placenta,. This was accomplished by monitoring cows 2 weeks before calving to 3 weeks after calving for any postpartum diseases using daily visual examination and automatically detected variables including activity, milk yield, milk components, lying behavior, feeding behavior, rumination time, …
On-Farm Utilization Of Precision Dairy Monitoring: Usefulness, Accuracy, And Affordability, Elizabeth A. Eckelkamp
On-Farm Utilization Of Precision Dairy Monitoring: Usefulness, Accuracy, And Affordability, Elizabeth A. Eckelkamp
Theses and Dissertations--Animal and Food Sciences
Precision dairy monitoring is used to supplement or replace human observation of dairy cattle. This study examined the value dairy producers placed on disease alerts generated from a precision dairy monitoring technology. A secondary objective was calculating the accuracy of technology-generated disease alerts compared against observed disease events. A final objective was determining the economic viability of investing in a precision dairy monitoring technology for detecting estrus and diseases.
A year-long observational study was conducted on four Kentucky dairy farms. All lactating dairy cows were equipped with a neck and leg tri-axial accelerometer. Technologies measured eating time, lying time, standing …
Differences In Behavioral And Physiological Variables Measured With Precision Dairy Monitoring Technologies Associated With Postpartum Diseases, I Ching Tsai
Theses and Dissertations--Animal and Food Sciences
The transition period is defined as the three weeks before and three weeks after the cow calves. Transition cow diseases are considered production diseases. Precision dairy monitoring (PDM) technologies measure physiological, behavioral, and production indicators on individual animals to improve management strategies and farm performance. The objective of the first study was to assess how hypocalcemia, hyperketonemia, and metritis affected variables measured by PDM technologies. The objective of the second study was to use variables from multiple commercially available PDM to examine alert performance generated from different analyses.
Precision Dairy Farming Technology Solutions For Detecting Dairy Cow Disease To Improve Dairy Cow Well-Being, Amanda Stone
Precision Dairy Farming Technology Solutions For Detecting Dairy Cow Disease To Improve Dairy Cow Well-Being, Amanda Stone
Theses and Dissertations--Animal and Food Sciences
Dairy cow health is multifactorial and complex. High producing dairy cows have been described as metabolic athletes, but metabolic and infectious diseases around calving affect many cows. These diseases have drastic negative effects on dairy cow well-being, milk production, and dairy farm economics. Early disease detection could potentially improve disease management, treatment, and future prevention techniques. The first objective of this research was to evaluate the use of activity, lying behavior, reticulorumen temperature, and rumination time determined by precision dairy farming technologies to detect transition cow diseases including hypocalcemia, ketosis, and metritis. The second objective was to evaluate the ability …
An Examination Of Milk Quality Effects On Milk Yield And Dairy Production Economics In The Southeastern United States, Derek T. Nolan
An Examination Of Milk Quality Effects On Milk Yield And Dairy Production Economics In The Southeastern United States, Derek T. Nolan
Theses and Dissertations--Animal and Food Sciences
Mastitis is one of the most costly diseases to dairy producers around the world with milk yield loss being the biggest contributor to economic losses. The objective of first study of this thesis was to determine the impacts of high somatic cell counts on milk yield loss. To accomplish this, over one million cow data records were collected from Southeastern US dairy herds. The objective of the second study was to determine optimum treatment cost of clinical mastitis by combining two economic modeling approaches used in animal health economics. The last objective of this thesis was to determine how much …
Behavioral Gait Change Characterization And Detection Using Precision Dairy Monitoring Technologies, Barbara Wadsworth Jones
Behavioral Gait Change Characterization And Detection Using Precision Dairy Monitoring Technologies, Barbara Wadsworth Jones
Theses and Dissertations--Animal and Food Sciences
Lameness is a painful disorder that decreases performance and is highly recognized as one of the most important health and welfare concerns for dairy cattle. Visual gait scoring is the most common way to detect gait change in dairy cattle. However, this is not only subjective, but is also time consuming and costly. A need to remove the subjective assessment of human observation exists. Therefore, automatic gait change detection for continuous monitoring by precision dairy monitoring technologies may be beneficial. The first objective of this research was to characterize behavior and production variables as cow gait changed to evaluate potential …
Monitoring Yogurt Culture Fermentation And Predicting Fermentation Endpoint With Fluorescence Spectroscopy, Timothey P. Mains, Frederick Alan Payne, Michael P. Sama
Monitoring Yogurt Culture Fermentation And Predicting Fermentation Endpoint With Fluorescence Spectroscopy, Timothey P. Mains, Frederick Alan Payne, Michael P. Sama
Biosystems and Agricultural Engineering Faculty Publications
Determination of the endpoint of yogurt culture fermentation is a process parameter that could benefit from automation. The feasibility of using a fluorescence sensor technology based on 280 nm excitation and 350 nm emission to predict the endpoint of yogurt culture fermentation was investigated and compared with the endpoint prediction from a near-infrared (880 nm) light backscatter sensor. Yogurt cultures with three levels of milk solids (8%, 10%, and 12%) and three temperatures (40°C, 43°C, and 46°C) were tested with three replications in a 3 x 3 factorial design (n = 27). Prediction models were developed for each optical measurement …
Incorporating Machine Vision In Precision Dairy Farming Technologies, Anthony N. Shelley
Incorporating Machine Vision In Precision Dairy Farming Technologies, Anthony N. Shelley
Theses and Dissertations--Electrical and Computer Engineering
The inclusion of precision dairy farming technologies in dairy operations is an area of increasing research and industry direction. Machine vision based systems are suitable for the dairy environment as they do not inhibit workflow, are capable of continuous operation, and can be fully automated. The research of this dissertation developed and tested 3 machine vision based precision dairy farming technologies tailored to the latest generation of RGB+D cameras. The first system focused on testing various imaging approaches for the potential use of machine vision for automated dairy cow feed intake monitoring. The second system focused on monitoring the gradual …
Assessment Of The Technical And Economic Potential Of Automated Estrus Detection Technologies For Dairy Cattle, Karmella Ann Dolecheck
Assessment Of The Technical And Economic Potential Of Automated Estrus Detection Technologies For Dairy Cattle, Karmella Ann Dolecheck
Theses and Dissertations--Animal and Food Sciences
Poor estrus detection can limit the reproductive performance of a dairy herd. One objective of this research was to evaluate an alternative method to traditional estrus detection in the form of automated monitoring technologies. To accomplish this, the first study considered the ability of automatically monitored parameters (activity, number of steps, lying bouts, lying time, feeding time, rumination time, and temperature) to detect estrus. A second study compared automated activity monitoring to timed artificial insemination as reproductive management strategies on commercial herds. The other objective of this research was to evaluate the economic potential of automated estrus detection technologies. This …
An Evaluation Of Precision Dairy Farming Technology Adoption, Perception, Effectiveness, And Use, Matthew Richard Borchers
An Evaluation Of Precision Dairy Farming Technology Adoption, Perception, Effectiveness, And Use, Matthew Richard Borchers
Theses and Dissertations--Animal and Food Sciences
Precision dairy farming technologies provide a variety of functions to dairy farmers. Little is known about dairy producer perception of these technologies. A study was performed to understand dairy producer perception of parameters monitored by precision dairy farming technologies. Calving has potential to be predicted using these same parameters and technologies. A second study was performed using two commercially marketed technologies in calving prediction. In order for these technologies to generate accurate and useful information for dairy farm use, they must accurately quantify these parameters. The final study evaluated the accuracy of five commercially marketed technologies in monitoring feeding, rumination, …
Assessing The Efficacy Of Automated Detection Of Estrus In Dairy Cattle, Lauren M. Mayo
Assessing The Efficacy Of Automated Detection Of Estrus In Dairy Cattle, Lauren M. Mayo
Theses and Dissertations--Animal and Food Sciences
The detection of estrus continues to be a primary factor contributing to poor reproductive performance in modern dairy cattle. The objectives of this research were 1) to evaluate performance of automated detection of estrus using a reference standard of ovulation detection with temporal progesterone patterns 2) to evaluate the efficacy of parameters measured by automated detection of estrus systems 3) to evaluate the efficacy of alerts generated by several commercially available systems used for automated detection of estrus and 4) to determine the differences in these parameters among cows with or without poor health conditions at the time of estrus. …
Algae Or Yeast Supplementation For Lactating Dairy Cows, Maegan E. Weatherly
Algae Or Yeast Supplementation For Lactating Dairy Cows, Maegan E. Weatherly
Theses and Dissertations--Animal and Food Sciences
The objective of the first study was to quantify the effects of feeding Schizochytrium sp. microalgae (SP-1, Alltech, Inc., Nicholasville, KY) on milk fat and DHA content. Eight cows were fed: 0, 100, 300, or 600 g of algae per day. Fat percentage was greater (P < 0.05) for cows on treatments 0 g and 100 g than for cows on treatments 300 g and 600 g (P < 0.05). Docosahexaenoic acid in milk was greater for cows on treatment 300 and 600 than for cows on treatment 0 and 100 (P < 0.05).
The objective of the second study was to assess yeast supplementation effects on high and low forage dairy cow diets. Four cows were assigned to 1 of 4 treatments: 1) low forage (LF), 2) low forage with 10 g/d yeast (Yea-Sacc®; Alltech Inc., Nicholasville, KY; LFY), 3) high …
Examining Vegetative Growth Of Cool-Season Forage Grasses For Dairy Cattle Grazing Preference, Eric D. Billman
Examining Vegetative Growth Of Cool-Season Forage Grasses For Dairy Cattle Grazing Preference, Eric D. Billman
Theses and Dissertations--Plant and Soil Sciences
The objective of this study was to determine dairy cattle preference amongst four species of cool-season forage grasses: eight orchardgrasses (Dactylis glomerata L.), five tall fescues [Schedonorus arundinaceus (Schreb.) Dumort.], five perennial ryegrasses (Lolium perenne L.), and six festuloliums [xFestulolium braunii (K. Richt.) A. Camus.]; 24 cultivars in total. Each grazing trial utilized four Holstein-Friesian heifers over six hours. Maturity differences were eliminated by having animals graze only vegetative material. After six grazing trials (three each in 2014 and 2015), consistent results in animal preference were not found; three of the six trials did show preference …