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Full-Text Articles in Life Sciences
Evaluating Biogenesis Of 5’-Tailed Mirtrons, Jonathan D. Hoover
Evaluating Biogenesis Of 5’-Tailed Mirtrons, Jonathan D. Hoover
Honors Theses
MicroRNAs are 22 nucleotide, non-coding RNAs that serve as substrates for Argonaute proteins to induce RNA interference pathways. Intron-derived miRNA precursors called “mirtrons” have been identified and classified primarily through deep sequencing methods. Unlike most miRNAs, mirtrons are derived from splicing events and also exhibit high levels of post-transcriptional nucleotide addition to hairpin precursors. Most relevant among these modifications is 3’ uridylation as it inhibits mirtron biogenesis in multiple model systems. Mirtrons may also possess additional nucleotides adjacent to the pre-miRNA hairpin at the 3’ and/or 5’ ends. These nucleotide “tails” are removed prior to Dicer cleaving the hairpin. In …
The Uas-Gal4 System In D. Melanogaster: An Insight Into The Influence Of Micrornas On The Developmental Pathways Of The Wing, Emily R. Wilson
The Uas-Gal4 System In D. Melanogaster: An Insight Into The Influence Of Micrornas On The Developmental Pathways Of The Wing, Emily R. Wilson
Honors Theses
By examining genetic pathways in D. melanogaster, a better understanding of the homologous regulatory mechanisms in humans can be utilized to further enhance knowledge of the roles of microRNA within development. This study utilizes the UAS-Gal4 system in order to produce a mutant phenotype capable of being visually studied and analyzed, focusing on the developmental pathway of the wing in D. melanogaster. Dissections of the wandering third instar larvae yielded wing disc tissue expressing the downregulation of loquacious and CG17386.