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Intracellular Signalling Crosstalk In The Differentiation Of F9 Cells Into Extraembryonic Endoderm, Tina Nicole Cuthbert
Intracellular Signalling Crosstalk In The Differentiation Of F9 Cells Into Extraembryonic Endoderm, Tina Nicole Cuthbert
Electronic Thesis and Dissertation Repository
Mouse F9 cells differentiate into primitive extraembryonic endoderm (PrE) with retinoic acid (RA) treatment, resulting in up-regulation of Gata6, which when translated directly activates Wnt6. Canonical Wnt signalling is required for PrE differentiation, and this, like most developmental processes, requires input from one or more additional pathways, including hedgehog (Hh). The Hh pathway is regulated by GATA6, and crosstalks positively/negatively with Wnt signalling. Ihh up-regulation in F9 cells accompanies PrE induction, but a role for GATA6 or Hh pathway activation in obligatory Wnt/ß-catenin signalling is not known. To address this, I show that RA induces Ihh and altered expression of …
Cell Signaling Crosstalk And Differentiation Of F9 Cells Into Extraembryonic Endoderm, Gurjoth Singh Deol
Cell Signaling Crosstalk And Differentiation Of F9 Cells Into Extraembryonic Endoderm, Gurjoth Singh Deol
Electronic Thesis and Dissertation Repository
Mouse F9 cells differentiate into primitive endoderm (PrE) when treated with retinoic acid (RA). During PrE differentiation the canonical Wnt signaling pathway is known to play an integral role in the process. In addition to Wnt signaling, there have been implications that the Hedgehog (Hh) pathway may also be involved. Previous results show that the Indian Hedgehog (Ihh) gene is upregulated during RA-induced differentiation; however further details of Hh signaling during PrE differentiation have yet to be discovered. A Gli-luciferase construct indicated that Hh signaling increases during RA-induced differentiation, implicating that the pathway is involved in PrE formation. Inhibiting …
Wnt Signalling During F9 Cell Differentiation, Gregory M. Golenia
Wnt Signalling During F9 Cell Differentiation, Gregory M. Golenia
Electronic Thesis and Dissertation Repository
Mouse F9 cells differentiate into primitive endoderm (PrE) when treated with retinoic acid (RA) and this is accompanied by the upregulation of Wnt6 and activation of the canonical WNT/β-catenin pathway. Previous studies have demonstrated the necessity of β-catenin-TCF/LEF transcription for primitive endoderm differentiation, however the Frizzled (FZD) receptor responsible for binding WNT6 and activating the canonical WNT pathway is not known. It was hypothesized that FZD7 is responsible for binding and transducing the WNT6 signal. Fzd7 mRNA was detected in undifferentiated and primitive endoderm cells, and its expression does not change significantly in response to RA. Moreover and contrary to …
Nucleoredoxin And Wnt Signaling In F9 Cells, Leanne Sandieson
Nucleoredoxin And Wnt Signaling In F9 Cells, Leanne Sandieson
Electronic Thesis and Dissertation Repository
Nucleoredoxin is a redox sensitive protein recently shown to be involved in the Wnt/β-catenin pathway through binding dishevelled. When Wnt is present, dishevelled prevents a destruction complex from degrading β-catenin. This facilitates the translocation of β-catenin into the nucleus where it binds to TCF-LEF to impart changes in gene expression. This study used mouse F9 cells as a model to examine whether or not nucleoredoxin is involved in augmenting the Wnt/β-catenin signaling required for extraembryonic endoderm differentiation. It was found that nucleoredoxin and three dishevelled isoforms were present in F9 cells. Furthermore, nucleoredoxin was found to bind to dishevelled-2, and …