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Analysis Of Human Sperm Chromatin Integrity, Silvina M. Bocca
Analysis Of Human Sperm Chromatin Integrity, Silvina M. Bocca
Theses and Dissertations in Biomedical Sciences
Determining potential maternal or paternal sources of abnormal chromosomal constitution gives opportunity for preconception genetic counseling. The most direct determination is achieved by analyzing the nuclear constitution of the gametes.
The present study evaluated the integrity of human spermatozoal nuclear material in the two condensation stages of chromatin and chromosomes. Original semen samples (ORI) and their swim-up fractions (SW, selected for motility) from men of known (donors) and unknown (patients) fertility were analyzed. The extent of chromatin condensation was assessed by light microscopy and flow cytometry during the time course of a chemically-induced decondensation reaction.
Motile spermatozoa were used to …