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Lysis Of The Rhizobiaceae, Craig Richard Baumrucker
Lysis Of The Rhizobiaceae, Craig Richard Baumrucker
Masters Theses
The following members of the Rhizobiaceae were tested for their lytic response to various treatments: Agrobacterium tumefaciens, Chromobacterium violaceum, Rhizobium phaseoli, Rhizobium trifolii, Rhizobium lupini, and Rhizobium japonicum. Lysis was observed in spent growth media and as a function of saline concentration, pH, buffer composition, disodium (ethylenedinitrilo) tetraacetate (EDTA) concentration, and lysozyme.
Cells were harvested by centrifugation, washed, and resuspended in various treatment solutions. The lytic response was followed spectrophotometrically.
The different responses of the six cultures was applied to a summation of reclassification proposals evolved through modern taxonomic techniques such as DNA homology …