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Full-Text Articles in Life Sciences
Characterizing The Heavy Metal Chelator, Tpen, As A Ca2+ Tool In The Mammalian Oocyte, Robert A. Agreda Mccaughin
Characterizing The Heavy Metal Chelator, Tpen, As A Ca2+ Tool In The Mammalian Oocyte, Robert A. Agreda Mccaughin
Masters Theses 1911 - February 2014
N,N,N’,N’-tetrakis-(2-Pyridylmethyl) ethylenediamine (TPEN) is a heavy metal chelator with high affinity for zinc. TPEN causes important responses in mammalian eggs. For example, these eggs are arrested at the MII stage by the Endogenous Mitotic Inhibitor 2 (Emi2), which prevents activation of the Anaphase Promoting Complex (APC) and degradation of Cyclin B. By chelating zinc, TPEN inactivates Emi2 and eggs undergo spontaneous exit of meiosis and egg activation. TPEN chelates Ca2+ with lower affinity, although in the Endoplasmic Reticulum (ER), where Ca2+ concentrations are high, TPEN may sequester Ca2+ preventing release into the cytoplasm. Initial exposure of TPEN …
Stiffness And Modulus And Independent Controllers Of Breast Cancer Metastasis, Dannielle Ryman
Stiffness And Modulus And Independent Controllers Of Breast Cancer Metastasis, Dannielle Ryman
Masters Theses 1911 - February 2014
One out of eight women in the United States will develop breast cancer during their lifetime. Ninety percent of cancer related deaths are due to metastasis. Metastasis is the biological process where individual or aggregate cancerous cells break away from the primary tumor site and colonize distant, non-adjacent locations throughout the body. It is my objectives to study how mechanical, topographical and biochemical cues affect metastatic breast cancer metastasis at an early developmental stage. ECM components have previously been shown to affect cell motility via ligand-receptor interactions, and physical cues, such as matrix stiffness and protein density. The primary tumor …