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Kif3a Tail Domain Phosphorylation Is Not Required For Ciliogenesis In Mouse Embryonic Fibroblasts, Ayoola Fasawe, Jessica M. Adams, Martin Engelke
Kif3a Tail Domain Phosphorylation Is Not Required For Ciliogenesis In Mouse Embryonic Fibroblasts, Ayoola Fasawe, Jessica M. Adams, Martin Engelke
Faculty Publications – Biological Sciences
Primary cilia are essential signaling organelles that protrude from most cells in the body. Heterodimeric kinesin-2 (KIF3A/KIF3B/KAP3) powers several intracellular transport processes, including intraflagellar transport (IFT), essential for ciliogenesis. A long-standing question is how a motor protein is differentially regulated for specific cargos. Since phosphorylation of the KIF3A tail domain was suggested to regulate the activity of kinesin-2 for ciliogenesis, similarly as for the cytosolic cargo N-Cadherin, we set out to map the phosphosites involved in this regulation. Using well-characterized Kif3a−/−; Kif3b−/− mouse embryonic fibroblasts, we performed ciliogenesis rescue assays with a library of phosphomimetic mutants comprising all …