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Articles 1 - 8 of 8
Full-Text Articles in Life Sciences
Phenotypic And Genotypic Characterization Of Escherichia Coli Isolated From Untreated Surface Waters, Kai F. Hung, Steven L. Daniel, Kristopher J. Janezic, Blake Ferry, Eric W. Hendricks, Brian A. Janiga, Tiffany Johnson, Samantha Murphy, Morgan E. Roberts, Sarah M. Scott, Alexandra N. Theisen
Phenotypic And Genotypic Characterization Of Escherichia Coli Isolated From Untreated Surface Waters, Kai F. Hung, Steven L. Daniel, Kristopher J. Janezic, Blake Ferry, Eric W. Hendricks, Brian A. Janiga, Tiffany Johnson, Samantha Murphy, Morgan E. Roberts, Sarah M. Scott, Alexandra N. Theisen
Faculty Research & Creative Activity
A common member of the intestinal microbiota in humans and animals is Escherichia coli. Based on the presence of virulence factors, E. coli can be potentially pathogenic. The focus of this study was to isolate E. coli from untreated surface waters (37 sites) in Illinois and Missouri and determine phenotypic and genotypic diversity among isolates. Water samples positive for fecal coliforms based on the Colisure® test were streaked directly onto Eosin Methylene Blue (EMB) agar (37°C) or transferred to EC broth (44.5°C). EC broth cultures producing gas were then streaked onto EMB agar. Forty-five isolates were identified as E. coli …
Phenotypic And Genotypic Characterization Of Escherichia Coli Isolated From Untreated Surface Waters, Kai Hung, Steven Daniel, Kristopher Janezic, Blake Ferry, Eric Hendricks, Brian Janiga, Tiffany Johnson, Samantha Murphy, Morgan Roberts, Sarah Scott, Alexandra Theisen
Phenotypic And Genotypic Characterization Of Escherichia Coli Isolated From Untreated Surface Waters, Kai Hung, Steven Daniel, Kristopher Janezic, Blake Ferry, Eric Hendricks, Brian Janiga, Tiffany Johnson, Samantha Murphy, Morgan Roberts, Sarah Scott, Alexandra Theisen
Faculty Research & Creative Activity
A common member of the intestinal microbiota in humans and animals is Escherichia coli. Based on the presence of virulence factors, E. coli can be potentially pathogenic. The focus of this study was to isolate E. coli from untreated surface waters (37 sites) in Illinois and Missouri and determine phenotypic and genotypic diversity among isolates. Water samples positive for fecal coliforms based on the Colisure® test were streaked directly onto Eosin Methylene Blue (EMB) agar (37°C) or transferred to EC broth (44.5°C). EC broth cultures producing gas were then streaked onto EMB agar. Forty-five isolates were identified as E. coli …
Intracellular Expression Of An Ice Nucleation Protein Reduces Cryoinjury In Insect Cells, Avril M. Harder
Intracellular Expression Of An Ice Nucleation Protein Reduces Cryoinjury In Insect Cells, Avril M. Harder
Student Honors Theses
Exposure of insect cells to subzero temperatures typically leads to cell membrane disruption and lethal intracellular ice formation. This study seeks to examine the cryoprotective value of trangenically expressing a bacterial ice nucleation protein (INP) in Spodoptera frugiperda (Sf-21) cells. The bacterium Pseudomonas syringae naturally produces a membrane-bound INP (inaZ), capable of structuring water and initiating ice formation at temperatures as high as -2 °C. I hypothesized that intracellular expression of an altered form of inaZ (PsINP) in Sf-21 cells will mediate highly regulated ice nucleation when cells are cooled to -80 °C in a slow, controlled manner, …
Using Comparative Genomics For Inquiry-Based Learning To Dissect Virulence Of Escherichia Coli O157:H7 And Yersinia Pestis, David J. Baumler, Lois M. Banta, Kai F. Hung, Jodi A. Schwarz, Eric L. Cabot, Jeremy D. Glasner, Nicole T. Perna
Using Comparative Genomics For Inquiry-Based Learning To Dissect Virulence Of Escherichia Coli O157:H7 And Yersinia Pestis, David J. Baumler, Lois M. Banta, Kai F. Hung, Jodi A. Schwarz, Eric L. Cabot, Jeremy D. Glasner, Nicole T. Perna
Faculty Research & Creative Activity
Genomics and bioinformatics are topics of increasing interest in undergraduate biological science curricula. Many existing exercises focus on gene annotation and analysis of a single genome. In this paper, we present two educational modules designed to enable students to learn and apply fundamental concepts in comparative genomics using examples related to bacterial pathogenesis. Students first examine alignments of genomes of Escherichia coli O157:H7 strains isolated from three food-poisoning outbreaks using the multiple-genome alignment tool Mauve. Students investigate conservation of virulence factors using the Mauve viewer and by browsing annotations available at the A Systematic Annotation Package for Community Analysis of …
Using Comparative Genomics For Inquiry-Based Learning To Dissect Virulence Of Escherichia Coli O157:H7 And Yersinia Pestis, David Baumler, Lois Banta, Kai Hung, Jodi Schwarz, Eric Cabot, Jeremy Glasner, Nicole Perna
Using Comparative Genomics For Inquiry-Based Learning To Dissect Virulence Of Escherichia Coli O157:H7 And Yersinia Pestis, David Baumler, Lois Banta, Kai Hung, Jodi Schwarz, Eric Cabot, Jeremy Glasner, Nicole Perna
Faculty Research & Creative Activity
Genomics and bioinformatics are topics of increasing interest in undergraduate biological science curricula. Many existing exercises focus on gene annotation and analysis of a single genome. In this paper, we present two educational modules designed to enable students to learn and apply fundamental concepts in comparative genomics using examples related to bacterial pathogenesis. Students first examine alignments of genomes of Escherichia coli O157:H7 strains isolated from three food-poisoning outbreaks using the multiple-genome alignment tool Mauve. Students investigate conservation of virulence factors using the Mauve viewer and by browsing annotations available at the A Systematic Annotation Package for Community Analysis of …
Molybdate Treatment And Sulfate Starvation Decrease Atp And Dna Levels In Ferroplasma Acidarmanus, Kai Hung
Molybdate Treatment And Sulfate Starvation Decrease Atp And Dna Levels In Ferroplasma Acidarmanus, Kai Hung
Faculty Research & Creative Activity
Sulfate is a primary source of sulfur for most microbes and in some prokaryotes it is used an electron acceptor. The acidophile Ferroplasma acidarmanus (strain fer1) requires a minimum of 150 mM of a sulfate-containing salt for growth. Sulfate is assimilated by F. acidarmanus into proteins and reduced to form the volatile organic sulfur compounds methanethiol and dimethyldisulfide. In the absence of sulfate, cell death occurs by an unknown mechanism. In this study, cell viability and genomic DNA and ATP contents of F. acidarmanus were monitored in response to the absence of sulfate or the presence of sulfate and the …
Annotation Of The Bacteriophage 933w Genome: An In-Class Interactive Web-Based Exercise, Kai F. Hung
Annotation Of The Bacteriophage 933w Genome: An In-Class Interactive Web-Based Exercise, Kai F. Hung
Faculty Research & Creative Activity
No abstract provided.
Molybdate Treatment And Sulfate Starvation Decrease Atp And Dna Levels In Ferroplasma Acidarmanus, Kai F. Hung
Molybdate Treatment And Sulfate Starvation Decrease Atp And Dna Levels In Ferroplasma Acidarmanus, Kai F. Hung
Faculty Research & Creative Activity
Sulfate is a primary source of sulfur for most microbes and in some prokaryotes it is used an electron acceptor. The acidophile Ferroplasma acidarmanus (strain fer1) requires a minimum of 150 mM of a sulfate-containing salt for growth. Sulfate is assimilated by F. acidarmanus into proteins and reduced to form the volatile organic sulfur compounds methanethiol and dimethyldisulfide. In the absence of sulfate, cell death occurs by an unknown mechanism. In this study, cell viability and genomic DNA and ATP contents of F. acidarmanus were monitored in response to the absence of sulfate or the presence of sulfate and the …